In Silico Identification of Potential Clovibactin-like Antibiotics Binding to Unique Cell Wall Precursors in Diverse Gram-Positive Bacterial Strains

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1724 - 1724

Published: Feb. 18, 2025

The rise in multidrug-resistant bacteria highlights the critical need for novel antibiotics. This study explores clovibactin-like compounds as potential therapeutic agents targeting lipid II, a crucial component bacterial cell wall synthesis, using silico techniques. A total of 2624 clovibactin analogs were sourced from PubChem database and screened ProTox 3.0 software based on their ADME-Tox properties, prioritizing candidates with favorable pharmacokinetic profiles minimal toxicity. Molecular docking protocols then employed to assess binding interactions selected II. Our analysis identified Compound 22 particularly promising candidate, exhibiting strong affinity, stable complex formation, high selectivity target. Binding energy analysis, conducted via molecular dynamics simulations, revealed highly negative value -25.50 kcal/mol 22, surpassing that underscoring its efficacy. In addition, was prioritized due exceptional affinity II suggesting lower likelihood adverse effects. These characteristics position candidate further pharmacological development. While our computational results are encouraging, experimental validation is essential confirm efficacy safety these compounds. not only advances understanding but also contributes ongoing efforts combat antimicrobial resistance through innovative antibiotic

Language: Английский

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Outbreak of High-Risk Clone ST323 Klebsiella pneumoniae Resistant to Ceftazidime–Avibactam Due to Acquisition of blaVEB-25 and to Cefiderocol Due to Mutated fiu Gene

Antibiotics, Journal Year: 2025, Volume and Issue: 14(3), P. 223 - 223

Published: Feb. 21, 2025

Background/Objectives: The incidence of Ceftazidime/Avibactam (CZA)-resistant Klebsiella pneumoniae isolate co-producing carbapenemase 2 (KPC-2) and Vietnamese extended-spectrum β-lactamase 25 (VEB-25) has been on the rise in Greece over past five years. This study investigates isolation ST323 K. isolates co-resistant to CZA cefiderocol (FDC) from colonized infected patients a single hospital Athens. Methods: CZA-resistant strains were isolated 5 ICU 27 December 2023 22 January 2024. Antimicrobial susceptibility was tested against panel agents. Whole-genome sequencing carried out identify acquired resistance genes mutations that associated with FDC resistance. Results: belonged harbored blaKPC-2 blaVEB-25. had minimum inhibitory concentration (MIC) >256 mg/L for 32 FDC, due disrupted catecholate siderophore receptor Fiu. blaVEB-25 located an IncC non-conjugative plasmid ~14 kb multidrug (MDR) region comprising 15 further genes. Transformation studies showed blaVEB-25-carrying provided most β-lactams tested, including CZA. remained susceptible carbapenems, imipenem/relebactam, meropenem/vaborbactam. citrate-dependent iron (III) uptake system (fecIRABCDE), which increased MIC ≤0.08 mg/L. Conclusions: gene plasmids are important contributors spread key antibiotic Strict infection control measures must be elaborated upon prevent extensively drug-resistant organisms such as those described here.

Language: Английский

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Amomum longiligulare polysaccharide 1 supplementation promotes the proliferation of jejunal epithelial cells in piglets by regulating jejunal metabolites

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 306, P. 141366 - 141366

Published: Feb. 22, 2025

Language: Английский

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Antimicrobial resistance and virulence in Klebsiella pneumoniae: a four-month study in Osogbo, Nigeria

Antimicrobial Stewardship & Healthcare Epidemiology, Journal Year: 2025, Volume and Issue: 5(1)

Published: Jan. 1, 2025

Abstract Objective: Antimicrobial resistance (AMR) is a growing global health crisis, with Klebsiella pneumoniae being key pathogen due to its multidrug (MDR). This study aimed investigate the profiles, demographic correlations, and molecular characteristics of MDR K. at UNIOSUN Teaching Hospital, Osogbo, Nigeria. Methods: From January April 2022, 99 clinical isolates ( ) were collected from various specimen types (blood, sputum, urine, wound, stool, oral cavity). Antibiotic susceptibility was assessed using Kirby-Bauer disk diffusion method, virulence genes analysed multiplex polymerase chain reaction. Results: All exhibited ceftriaxone, cefotaxime, colistin, high observed for cefepime carbapenems (meropenem, imipenem, ertapenem). Molecular characterization revealed presence K1, K2, mrkD in 15 isolates, while other tested fimH, ramA, traT, K3, K5 not detected. Significant associations identified between patterns factors, including age sex, highlighting potential vulnerabilities specific populations. Conclusions: underscores alarming prevalence aligns trends rising AMR. Addressing these challenges requires targeted antimicrobial stewardship programs, infection control measures, public education, enhanced surveillance systems. Incorporating testing novel therapeutic agents future research crucial developing effective containment strategies preserving antibiotic efficacy.

Language: Английский

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Facile Synthesis of Carbon Dots and Their Potential Applications Against Multidrug Resistance (MDR)

BioNanoScience, Journal Year: 2025, Volume and Issue: 15(2)

Published: March 19, 2025

Language: Английский

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Current access, availability and use of antibiotics in primary care among key low- and middle-income countries and the policy implications

Expert Review of Anti-infective Therapy, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

Introduction Antimicrobial resistance (AMR) poses a significant threat, particularly in low- and middle-income countries (LMICs), exacerbated by inappropriate antibiotic use, access to quality antibiotics weak antimicrobial stewardship (AMS). There is need review current evidence on access, AMR, primary care across key countries.Areas covered: This narrative analyses publications from 2018 2024 regarding availability use of appropriate antibiotics.

Language: Английский

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The Emergence and Prevalence of Antibiotic Resistance in Genus Enterococcus and Their Implications on Probiotics

IntechOpen eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

The genus Enterococcus has a ubiquitous distribution and is found in all possible places of microorganisms’ existence. Due to their unique properties, species also survives harsh environmental conditions, the guts animals, extreme industrial processing settings. These properties make them an important microbe our daily lives. Currently, enterococcal are used food, pharmaceuticals, cheeses, leather, etc., contribute many other aspects. probiotic potential explored, good number probiotics commercialized. Unfortunately, emergence antibiotic resistance limits valuable contributions, hence, they now treated as opportunistic pathogens, being so-called common commensals. Both intrinsic acquired identified species. Generally, E. faecium faecalis, which considered more resistant virulent, respectively, responsible for than 80% infections. situation became worse when started develop last-resort antibiotics, like linezolid daptomycin. Enterococcus, having extensive applications lives, thus appeals studying profiling taking action before any disease outbreaks. Besides mortality morbidity, enterococci greatly affects probiotics. Probiotics free from may act reservoir harboring genes have transfer commensal pathogenic microbes. This chapter aims provide comprehensive analysis antimicrobial its implications on

Language: Английский

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Phytochemical screening, UPLC analysis, evaluation of synergistic antioxidant and antibacterial efficacy of three medicinal plants used in Kinshasa, D.R. Congo

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 24, 2025

Plant extracts are used worldwide due to their biologically active compounds, which support food preservation and help combat various diseases through antimicrobial antioxidant properties. In the capital city of Democratic Republic Congo, an ethnobotanical survey revealed use Dysphania ambrosioides (L.) Mosyakin & Clemants, Ocimum gratissimum L. Tetradenia riparia (Hochst.) Codd, often in combination, for treating oral microbial diseases. While these plants have been widely studied individually; combined potential has not investigated. The present research aims explore phytochemical composition, synergistic antimicrobial, different from three mentioned plants. Phytochemical composition decocted percolated was determined using qualitative analysis ultra-performance liquid chromatography quadrupole time flight tandem mass spectrometry (UPLC-QTOF-MS). Antimicrobial activity assessed broth dilution method, while evaluated DPPH method. For studies, were tested against Staphylococcus aureus, Escherichia coli Pseudomonas aeruginosa. Qualitative presence alkaloids, saponins, flavonoids, iridoids, anthraquinones all plant extracts. methanolic extract T. exhibited highest phenolic content (299.146 ± 0.143 mg GAE/g extract), O. had flavonoid (138.256 0.277 QE/g extract). UPLC identified several metabolites including rosmarinic acid, cirsimaritin, xanthomicrol kaempferol derivatives. Rosmarinic acid consistently across combinations, other flavonoids such as apigenin 7-glycosides, kaempferitin luteolin 7-O-glucoside, detected specific combinations. higher than extracts, with showing (11.744 0.584 µg/mL), followed by (12.916 0.972 µg/mL). combinations demonstrated (CI = 0.57). Amongst antibacterial observed S. aureus (MIC 500 combination additive (FICI 1). aim this study primarily evaluate combinatory reliable sources antimicrobials antioxidants treatment future. provides evidence riparia. These results suggest that promising natural agents, application pharmaceutical industries combating infectious

Language: Английский

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Citrus Symphony: Antibacterial Efficacy of Essential Oils Against Aeromonas jandaei

Current Microbiology, Journal Year: 2025, Volume and Issue: 82(5)

Published: March 25, 2025

Language: Английский

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Дослідження впливу біогальванічного струму на біоплівкоутворення полірезистентних клінічних штамів умовно-патогенних збудників ранової інфекції

Ukrainian Journal of Military Medicine, Journal Year: 2025, Volume and Issue: 6(1), P. 139 - 146

Published: March 31, 2025

Вступ. Стандартне лікування антибіотиками є неефективним щодо інфекцій, пов’язаних із біоплівками та полірезистентними збудниками. Мета. Вивчення впливу струмів низької інтенсивності без зовнішніх джерел живлення на біоплівкоутворювальні властивості полірезистентних збудників інфекційних ускладнень бойових ран опіків. Матеріали і методи. Дію біогальванічного струму досліджували штамів Staphylococcus aureus, Acinetobacter baumannii, Pseudomonas aeruginosa, які було виділено з інфікованих осколкових, мінно-вибухових опіків різної локалізації. Суспензії клінічних у фізіологічному розчині м’ясо-пептонному бульйоні (МПБ; ≈1.6 × 109 КУО/мл) попередньо піддавали дії (46-50 мкА - 54-60 – МПБ) впродовж 48 год. Біоплівкоутворюючі визначали методом Крістенсена, який ґрунтується адгезії мікроорганізмів до поверхні пластикового планшету подальшою їх фіксацією, фарбуванням кристалічним фіолетовим спектрофотометричним аналізом фарбника, що розчинився спирті. Вимірювали оптичну густину (ОГ) за довжини хвилі 620 нм. Результати. В умовах гальванічного спостерігався біоелектричний ефект, проявлявся зниженням біоплівкоутворювальних властивостей поліантибіотикорезистентних S. A. baumannii P. aeruginosa як після обробки в поживного середовища (на 28,26 %, 24,82 % 11,89 відповідно) фізіологічного розчину 33,18 28,0 16,31 відповідно). Для струмом МПБ виявлено достовірну різницю біоплівкоутворення 1,38 рази (p <0,001) 1,33 = 0,01) порівнянні контролем значення оптичної густини становили відповідно 0,223±0,008 одиниць ОГ (ОГГ) 0,238±0,010 ООГ, а контролі 0,311±0,019 ООГ 0,317±0,019 ООГ. Під впливом ізотонічному показники, характеризували здатність S.aureus, A.baumannii P.aeruginosa були статистично достовірно нижчими 1,19-1,49 0,203±0,006 <0,001), 0,206±0,013 (p=0,05) 0,364±0,020 (p<0,05) відповідно, (0,304±0,020 0,286±0,021 0,435±0,027 Висновки. Безперервна дія поживному знижує свідчить про додаткові терапевтичні при лікуванні хворих інфекційними ускладненнями, спричиненими біоплівкоутворюючими мікроорганізмами.

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