Antimicrobial Resistance Patterns and Biofilm Analysis via Sonication in Intensive Care Unit Patients at a County Emergency Hospital in Romania DOI Creative Commons
Ioana Roxana Codru, Bogdan Ioan Vintilă, Alina Simona Bereanu

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(2), P. 161 - 161

Published: Jan. 25, 2025

Background/Objectives: Ventilator-associated pneumonia (VAP) remains a critical challenge in ICU settings, often driven by the biofilm-mediated bacterial colonization of endotracheal tubes (ETTs). This study investigates antimicrobial resistance patterns and biofilm dynamics patients, focusing on microbial trends tracheal aspirates tube biofilms at county emergency hospital Romania. Methods: We conducted longitudinal analysis patients requiring mechanical ventilation for more than 48 h. Tracheal ETT were collected three key time points: T1 (baseline), T2 (48 h post-intubation with replacement), T3 (92–100 post-T2); these analyzed using sonication microbiological techniques to assess patterns. Results: In total 30 bacteria from ESKAPEE group (e.g., Klebsiella pneumoniae, Acinetobacter baumannii, Staphylococcus aureus) dominated microbiota, increasing their prevalence over time. Resistance carbapenems, colistin, vancomycin was notably observed, particularly among K. pneumoniae A. baumannii. Biofilm revealed high persistence rates emergence multidrug-resistant strains, underscoring role ETTs as reservoirs resistant pathogens. The replacement correlated shift composition reduced biofilm-associated contamination. Conclusions: highlights temporal evolution limited number (30 patients). findings support implementing routine management strategies, including scheduled replacements advanced biofilm-disruption techniques, mitigate VAP risk improve patient outcomes.

Language: Английский

Antimicrobial Resistance Patterns and Biofilm Analysis via Sonication in Intensive Care Unit Patients at a County Emergency Hospital in Romania DOI Creative Commons
Ioana Roxana Codru, Bogdan Ioan Vintilă, Alina Simona Bereanu

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(2), P. 161 - 161

Published: Jan. 25, 2025

Background/Objectives: Ventilator-associated pneumonia (VAP) remains a critical challenge in ICU settings, often driven by the biofilm-mediated bacterial colonization of endotracheal tubes (ETTs). This study investigates antimicrobial resistance patterns and biofilm dynamics patients, focusing on microbial trends tracheal aspirates tube biofilms at county emergency hospital Romania. Methods: We conducted longitudinal analysis patients requiring mechanical ventilation for more than 48 h. Tracheal ETT were collected three key time points: T1 (baseline), T2 (48 h post-intubation with replacement), T3 (92–100 post-T2); these analyzed using sonication microbiological techniques to assess patterns. Results: In total 30 bacteria from ESKAPEE group (e.g., Klebsiella pneumoniae, Acinetobacter baumannii, Staphylococcus aureus) dominated microbiota, increasing their prevalence over time. Resistance carbapenems, colistin, vancomycin was notably observed, particularly among K. pneumoniae A. baumannii. Biofilm revealed high persistence rates emergence multidrug-resistant strains, underscoring role ETTs as reservoirs resistant pathogens. The replacement correlated shift composition reduced biofilm-associated contamination. Conclusions: highlights temporal evolution limited number (30 patients). findings support implementing routine management strategies, including scheduled replacements advanced biofilm-disruption techniques, mitigate VAP risk improve patient outcomes.

Language: Английский

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