Beyond the fever: shotgun metagenomic sequencing of stool unveils pathogenic players in HIV-infected children with non-malarial febrile illness DOI Creative Commons
Patricia Nabisubi, Stephen Kanyerezi, Grace Kebirungi

et al.

BMC Infectious Diseases, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 21, 2025

Non-malarial febrile illnesses (NMFI) pose significant challenges in HIV-infected children, often leading to severe complications and increased morbidity. While traditional diagnostic approaches focus on specific pathogens, shotgun metagenomic sequencing offers a comprehensive tool explore the microbial landscape underlying NMFI this vulnerable population ensuring effective management. In study, we employed metagenomics analyse stool samples from children at Baylor Children's Clinic Uganda presenting with non-malarial illness. Samples were collected subjected DNA extraction Molecular Genomics Laboratory, Makerere University followed by Chan Zuckerberg Biohub San Francisco. Bioinformatics analysis was conducted identify characterise composition potential pathogenic taxa associated using CZID pipeline. Our findings reveal diverse array of NMFI. Importantly, revealed potentially players including Trichomonas vaginalis, Candida albicans, Giardia, Bacteroides patient population. This sheds light complexities interactions that underpin illness group. Taxonomic profiling identified recognised pathogens comorbidities possible new correlations NMFI, shedding pathophysiology fever children. Shotgun is valuable method for understanding gut providing approach pathogen identification characterisation. By revealing actors beyond fever, work demonstrates how may improve our knowledge infectious groups inspire targeted therapies better clinical care outcomes.

Language: Английский

Beyond the fever: shotgun metagenomic sequencing of stool unveils pathogenic players in HIV-infected children with non-malarial febrile illness DOI Creative Commons
Patricia Nabisubi, Stephen Kanyerezi, Grace Kebirungi

et al.

BMC Infectious Diseases, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 21, 2025

Non-malarial febrile illnesses (NMFI) pose significant challenges in HIV-infected children, often leading to severe complications and increased morbidity. While traditional diagnostic approaches focus on specific pathogens, shotgun metagenomic sequencing offers a comprehensive tool explore the microbial landscape underlying NMFI this vulnerable population ensuring effective management. In study, we employed metagenomics analyse stool samples from children at Baylor Children's Clinic Uganda presenting with non-malarial illness. Samples were collected subjected DNA extraction Molecular Genomics Laboratory, Makerere University followed by Chan Zuckerberg Biohub San Francisco. Bioinformatics analysis was conducted identify characterise composition potential pathogenic taxa associated using CZID pipeline. Our findings reveal diverse array of NMFI. Importantly, revealed potentially players including Trichomonas vaginalis, Candida albicans, Giardia, Bacteroides patient population. This sheds light complexities interactions that underpin illness group. Taxonomic profiling identified recognised pathogens comorbidities possible new correlations NMFI, shedding pathophysiology fever children. Shotgun is valuable method for understanding gut providing approach pathogen identification characterisation. By revealing actors beyond fever, work demonstrates how may improve our knowledge infectious groups inspire targeted therapies better clinical care outcomes.

Language: Английский

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