Thiolutin Is a Potential Fungicide for Controlling Phytophthora nicotianae and Its Mechanistic Study DOI
Gang Xu, Xing Huang,

Yanqiong Liang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 14, 2025

Phytophthora nicotianae is a destructive plant pathogen that causes significant agricultural losses, with current chemical control strategies leading to resistance and environmental concerns. This study identified thiolutin, dithiopyrrolone antibiotic from Streptomyces luteireticuli ASG80, as potent antifungal agent against multiple species. Thiolutin exhibited strong in vitro activity, an EC50 value of 0.8266 μg/mL, significantly reduced disease incidence abundance vivo, outperforming conventional fungicides such metalaxyl. Transcriptomic analysis revealed downregulation key genes energy metabolism pathways, including mitochondrial function oxidative phosphorylation, indicating impaired production cellular metabolism. Microscopic biochemical analyses further demonstrated thiolutin disrupts cell membrane integrity, induces reactive oxygen species accumulation, damages mitochondria, ultimately disruption fungal programmed death. These findings highlight thiolutin's potential environmentally friendly alternative for managing Phytophthora-induced diseases.

Language: Английский

Thiolutin Is a Potential Fungicide for Controlling Phytophthora nicotianae and Its Mechanistic Study DOI
Gang Xu, Xing Huang,

Yanqiong Liang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 14, 2025

Phytophthora nicotianae is a destructive plant pathogen that causes significant agricultural losses, with current chemical control strategies leading to resistance and environmental concerns. This study identified thiolutin, dithiopyrrolone antibiotic from Streptomyces luteireticuli ASG80, as potent antifungal agent against multiple species. Thiolutin exhibited strong in vitro activity, an EC50 value of 0.8266 μg/mL, significantly reduced disease incidence abundance vivo, outperforming conventional fungicides such metalaxyl. Transcriptomic analysis revealed downregulation key genes energy metabolism pathways, including mitochondrial function oxidative phosphorylation, indicating impaired production cellular metabolism. Microscopic biochemical analyses further demonstrated thiolutin disrupts cell membrane integrity, induces reactive oxygen species accumulation, damages mitochondria, ultimately disruption fungal programmed death. These findings highlight thiolutin's potential environmentally friendly alternative for managing Phytophthora-induced diseases.

Language: Английский

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