Unlocking the Potential of Metagenomics with the PacBio High-Fidelity Sequencing Technology DOI Creative Commons

Yanhua Han,

J. He, Minghui Li

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2482 - 2482

Published: Dec. 2, 2024

Traditional methods for studying microbial communities have been limited due to difficulties in culturing and sequencing all species. Recent advances third-generation technologies, particularly PacBio’s high-fidelity (HiFi) sequencing, significantly advanced metagenomics by providing accurate long-read sequences. This review explores the role of HiFi overcoming limitations previous methods, including high error rates fragmented assemblies. We discuss benefits applications across various environments, such as human gut soil, which provides broader context further exploration. Key studies are discussed highlight sequencing’s ability recover complete coherent genomes from complex microbiomes, showcasing its superior accuracy continuity compared other technologies. Additionally, we explore potential quantitative analysis, well detection single nucleotide variations (SNVs) structural (SVs). PacBio is establishing a new benchmark metagenomics, with enhance our understanding ecology drive forward advancements both environmental clinical applications.

Language: Английский

Unlocking the Potential of Metagenomics with the PacBio High-Fidelity Sequencing Technology DOI Creative Commons

Yanhua Han,

J. He, Minghui Li

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2482 - 2482

Published: Dec. 2, 2024

Traditional methods for studying microbial communities have been limited due to difficulties in culturing and sequencing all species. Recent advances third-generation technologies, particularly PacBio’s high-fidelity (HiFi) sequencing, significantly advanced metagenomics by providing accurate long-read sequences. This review explores the role of HiFi overcoming limitations previous methods, including high error rates fragmented assemblies. We discuss benefits applications across various environments, such as human gut soil, which provides broader context further exploration. Key studies are discussed highlight sequencing’s ability recover complete coherent genomes from complex microbiomes, showcasing its superior accuracy continuity compared other technologies. Additionally, we explore potential quantitative analysis, well detection single nucleotide variations (SNVs) structural (SVs). PacBio is establishing a new benchmark metagenomics, with enhance our understanding ecology drive forward advancements both environmental clinical applications.

Language: Английский

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