Synergistic activity of dispersin B and benzoyl peroxide against Cutibacterium acnes/Staphylococcus epidermidis dual-species biofilms DOI Creative Commons
Jeffrey B. Kaplan,

Anna Muzaleva,

Miloslav Sailer

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(3), P. e0320662 - e0320662

Published: March 27, 2025

Cutibacterium acnes plays a key role in the development of acne vulgaris, with biofilm formation contributing to its persistence and resistance antimicrobial treatments. A critical component C. biofilms is poly- N -acetylglucosamine (PNAG), an exopolysaccharide that facilitates both stability biocide resistance. This study evaluated efficacy PNAG-degrading enzyme dispersin B enhancing susceptibility benzoyl peroxide (BP), common anti-acne agent. Dual-species Staphylococcus epidermidis , which has been shown promote growth under aerobic conditions, were cultivated glass tubes treated (5–80 µg/mL), BP (0.1–2.5%), or combination both. Dispersin alone reduced colony-forming units (CFUs) by 1–2 log units. However, sequential treatment followed achieved synergistic effect, yielding >6-log reduction CFUs. Remarkably, concentrations as low 5 µg/mL combined 0.5% efficiently eradicated from dual-species biofilms. These findings highlight protective PNAG against demonstrate potential adjunctive therapy enhance treatment.

Language: Английский

Synergistic activity of dispersin B and benzoyl peroxide against Cutibacterium acnes/Staphylococcus epidermidis dual-species biofilms DOI Creative Commons
Jeffrey B. Kaplan,

Anna Muzaleva,

Miloslav Sailer

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(3), P. e0320662 - e0320662

Published: March 27, 2025

Cutibacterium acnes plays a key role in the development of acne vulgaris, with biofilm formation contributing to its persistence and resistance antimicrobial treatments. A critical component C. biofilms is poly- N -acetylglucosamine (PNAG), an exopolysaccharide that facilitates both stability biocide resistance. This study evaluated efficacy PNAG-degrading enzyme dispersin B enhancing susceptibility benzoyl peroxide (BP), common anti-acne agent. Dual-species Staphylococcus epidermidis , which has been shown promote growth under aerobic conditions, were cultivated glass tubes treated (5–80 µg/mL), BP (0.1–2.5%), or combination both. Dispersin alone reduced colony-forming units (CFUs) by 1–2 log units. However, sequential treatment followed achieved synergistic effect, yielding >6-log reduction CFUs. Remarkably, concentrations as low 5 µg/mL combined 0.5% efficiently eradicated from dual-species biofilms. These findings highlight protective PNAG against demonstrate potential adjunctive therapy enhance treatment.

Language: Английский

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