Genome mining identifies a diversity of natural product biosynthetic capacity in human respiratory Corynebacterium strains DOI Creative Commons
Ashley L. Cunningham, Ilya Y. Zhbannikov, Rachel A. Myers

et al.

mSphere, Journal Year: 2025, Volume and Issue: unknown

Published: May 21, 2025

ABSTRACT Corynebacterium species, integral to the healthy human upper respiratory tract (URT) microbiota, remain underexplored in microbial genomics for their potential promote health and exclude pathobionts. This genomic study investigated diversity capacity natural product synthesis within these as indicated by biosynthetic gene clusters (BGCs). We aimed map quantify BGC a contemporary collection of strains, representative prevalence elucidate intra- interspecies variation content. The outcomes this research could reveal key factors maintaining ecological balance identify novel antimicrobial agents targeting Employing an silico approach, we analyzed strains non-diphtheriae species reference genomes through genome sequencing antiSMASH6 analysis. Among 161 genomes, identified 672 BGCs, 495 which were unique, including polyketide synthase, non-ribosomal peptide synthetase, ribosomally synthesized post-translationally modified peptide, siderophore families. To understand how compared other bacteria, then downloaded from eight that are associated with URT conducted searches. found despite compact possess multitude predicted exceeding BGCs multiple bacteria. lays foundation future functional studies on role microbiome discovery therapeutics derived bacterial genus. IMPORTANCE Bacterial secondary metabolites, produced enzymes encoded clusters, ecologically important communication competition nutrient-scarce environments historically rich source antibiotics medications. Human-associated abundant tract, understudied evidence roles promoting preventing pathobiont colonization. Through mining large isolated publicly available our suggests have high harbor wide range cluster These findings substantially expand current knowledge regarding production metabolites human-associated species. Our also foundations understanding interact discovering biotherapeutics.

Language: Английский

Autonomous Defense Based on Biogenic Nanoparticle Formation in Daunomycin-Producing Streptomyces DOI Creative Commons
K. Beneš, Vladislav Čurn, Baveesh Pudhuvai

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 107 - 107

Published: Jan. 8, 2025

Daunomycin is a chemotherapeutic agent widely used for the treatment of leukemia, but its toxicity toward healthy dividing cells limits clinical use and production by fermentation. Herein, we describe development specialized cultivation medium daunomycin production, including shift to oil rather than sugar as primary carbon source. This achieved an almost threefold increase in yields, reaching 5.5-6.0 g/L. produced oil-based was predominantly found solid sediment, whereas that sugar-based mostly soluble. The thus induces autonomous daunomycin-resistance mechanism involving biogenic nanoparticle formation. characterization nanoparticles confirmed incorporation iron daunomycin, indicating this approach has potential mitigate cytotoxicity while improving yields. presence proteins associated with homeostasis oxidative stress responses revealed ability strain adapt high concentrations. Our findings provide insight into mechanisms formation optimization processes. Further investigation will help refine microbial systems also broaden application similar strategies synthesis other therapeutically important compounds.

Language: Английский

Citations

0
Coordinated Regulation of Propionyl-CoA Carboxylase Subunits Drives Precursor Flux Optimization in Spinosad Production DOI

Ziyuan Xia,

Xiaomin Li, Xirong Liu

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Genome mining identifies a diversity of natural product biosynthetic capacity in human respiratory Corynebacterium strains DOI Creative Commons
Ashley L. Cunningham, Ilya Y. Zhbannikov, Rachel A. Myers

et al.

mSphere, Journal Year: 2025, Volume and Issue: unknown

Published: May 21, 2025

ABSTRACT Corynebacterium species, integral to the healthy human upper respiratory tract (URT) microbiota, remain underexplored in microbial genomics for their potential promote health and exclude pathobionts. This genomic study investigated diversity capacity natural product synthesis within these as indicated by biosynthetic gene clusters (BGCs). We aimed map quantify BGC a contemporary collection of strains, representative prevalence elucidate intra- interspecies variation content. The outcomes this research could reveal key factors maintaining ecological balance identify novel antimicrobial agents targeting Employing an silico approach, we analyzed strains non-diphtheriae species reference genomes through genome sequencing antiSMASH6 analysis. Among 161 genomes, identified 672 BGCs, 495 which were unique, including polyketide synthase, non-ribosomal peptide synthetase, ribosomally synthesized post-translationally modified peptide, siderophore families. To understand how compared other bacteria, then downloaded from eight that are associated with URT conducted searches. found despite compact possess multitude predicted exceeding BGCs multiple bacteria. lays foundation future functional studies on role microbiome discovery therapeutics derived bacterial genus. IMPORTANCE Bacterial secondary metabolites, produced enzymes encoded clusters, ecologically important communication competition nutrient-scarce environments historically rich source antibiotics medications. Human-associated abundant tract, understudied evidence roles promoting preventing pathobiont colonization. Through mining large isolated publicly available our suggests have high harbor wide range cluster These findings substantially expand current knowledge regarding production metabolites human-associated species. Our also foundations understanding interact discovering biotherapeutics.

Language: Английский

Citations

0