Multidrug-Resistant Extraintestinal Pathogenic Escherichia coli Exhibits High Virulence in Calf Herds: A Case Report DOI Creative Commons
Didi Zhu, Xinrui Li, Tengfei Ma

et al.

Microbiology Research, Journal Year: 2025, Volume and Issue: 16(3), P. 59 - 59

Published: Feb. 28, 2025

Extraintestinal pathogenic Escherichia coli (ExPEC) is a group of strains that can cause severe infectious diseases outside the gastrointestinal tract, such as urinary tract infections, meningitis, septicemia, etc. We report case calf herd infection by ExPEC with high rates morbidity and mortality. The research purpose this study was to thoroughly investigate characteristics responsible for infection. Specifically, we aimed understand mechanisms underlying its multidrug resistance pathogenicity. Clinical samples were collected isolation identification ExPECs, cultured on MacConkey agar, further tested PCR uidA gene, 16S rRNA gene sequencing, adhesion patterns HEp-2 cells. antimicrobial activity determined using disk diffusion method according & Laboratory Standards Institute (CLSI) guidelines. pathogenicity assessed through experimental Kunming mice, tracking their survival weight changes, performing autopsies bacterial counts histopathological analysis. Additionally, whole-genome sequencing (WGS) comprehensive analysis performed, including multilocus sequence typing (MLST), serotyping, drug-resistance analysis, virulence factor metabolic pathway enrichment various online tools databases. An strain named RZ-13 identified ST345 O134: H21. Among 14 antibiotics tested, 13 showed resistance, indicating multidrug-resistant (MDR) bacterium. mice proved greater than CICC 24186. WGS revealed presence 28 antibiotic genes 86 virulence-related in genome strain, corroborating clinical manifestations MDR Our isolated RZ-13, strong This first leading mortality herds China, underscoring need rational use reduce risk generation transmission bacteria from food-producing animals ensure food safety public health.

Language: Английский

Innovative Antibiotic Therapies for Carbapenem-Resistant Gram-Negative Bacterial Infections: Clinical Efficacy, Safety, and Comparative Studies DOI Creative Commons
Majid Eslami,

Amirabbas Safaripour,

Seyedeh Zahra Banihashemian

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 295 - 295

Published: Jan. 29, 2025

This review provides an overview of recent research and advancements in infection prevention the treatment drug-resistant bacterial diseases. Cefiderocol, a novel siderophore cephalosporin, has demonstrated effectiveness against carbapenem-resistant bacteria such as Pseudomonas aeruginosa Acinetobacter baumannii. Clinical trials, including APEKS-NP CREDIBLE-CR, affirm its efficacy for hospital-acquired pneumonia (HAP) but highlight concerns over increased mortality due to severe renal complications. Cefiderocol shown superior outcomes complicated urinary tract infections (cUTI) compared imipenem–cilastatin. A comparison colistin monotherapy versus combination therapy with meropenem revealed no significant improvement clinical noted delays resistance development. Colistin–rifampicin showed potential benefits colistin-resistant baumannii, although results were not statistically significant. SPR206, polymyxin derivative, durlobactam, β-lactamase inhibitor, show promise addressing these resistant strains, durlobactam demonstrating sulbactam Additional studies investigated antibiotic strategies infections, cefoperazone–sulbactam tigecycline, examined infection-prevention surgical settings, comparing chlorhexidine–alcohol povidone–iodine. highlights importance optimizing regimens infection-control measures across various healthcare neonatology care.

Language: Английский

Citations

0
Multidrug-Resistant Extraintestinal Pathogenic Escherichia coli Exhibits High Virulence in Calf Herds: A Case Report DOI Creative Commons
Didi Zhu, Xinrui Li, Tengfei Ma

et al.

Microbiology Research, Journal Year: 2025, Volume and Issue: 16(3), P. 59 - 59

Published: Feb. 28, 2025

Extraintestinal pathogenic Escherichia coli (ExPEC) is a group of strains that can cause severe infectious diseases outside the gastrointestinal tract, such as urinary tract infections, meningitis, septicemia, etc. We report case calf herd infection by ExPEC with high rates morbidity and mortality. The research purpose this study was to thoroughly investigate characteristics responsible for infection. Specifically, we aimed understand mechanisms underlying its multidrug resistance pathogenicity. Clinical samples were collected isolation identification ExPECs, cultured on MacConkey agar, further tested PCR uidA gene, 16S rRNA gene sequencing, adhesion patterns HEp-2 cells. antimicrobial activity determined using disk diffusion method according & Laboratory Standards Institute (CLSI) guidelines. pathogenicity assessed through experimental Kunming mice, tracking their survival weight changes, performing autopsies bacterial counts histopathological analysis. Additionally, whole-genome sequencing (WGS) comprehensive analysis performed, including multilocus sequence typing (MLST), serotyping, drug-resistance analysis, virulence factor metabolic pathway enrichment various online tools databases. An strain named RZ-13 identified ST345 O134: H21. Among 14 antibiotics tested, 13 showed resistance, indicating multidrug-resistant (MDR) bacterium. mice proved greater than CICC 24186. WGS revealed presence 28 antibiotic genes 86 virulence-related in genome strain, corroborating clinical manifestations MDR Our isolated RZ-13, strong This first leading mortality herds China, underscoring need rational use reduce risk generation transmission bacteria from food-producing animals ensure food safety public health.

Language: Английский

Citations

0