In Vitro Activity of Bacteriophages Against Ocular Methicillin-resistant S. aureus Isolates Collected in the US DOI Creative Commons
C.M. André, Mathieu Médina, Camille Kolenda

et al.

Ophthalmology and Therapy, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Methicillin-resistant Staphylococcus aureus (MRSA) is a leading cause of sight-threatening infections in the US. These strains pose significant challenge managing ocular infections, as they frequently exhibit resistance to first-line empirical antibiotics. To assess potential bacteriophages innovative topical therapies for treatment recalcitrant we evaluated vitro antimicrobial activity set anti-S. phages against collection MRSA clinical isolates collected The host range six (V4SA2, V1SA9, V1SA12, V1SA19, V1SA20 and V1SA22) was assessed using spot assay on panel 50 multidrug-resistant (MDR) selected be representative clones circulating Subsequently, liquid culture-based performed three most active different multiplicity infection (MOI 10–2, 1 or 100 phages/bacteria). In total, 90.0% bacterial were susceptible at least one phages. showed that V1SA22 had broadest spectrum, being 86%, 84% 82% isolates, respectively, including MDR-MRSA CC5 community-associated CC8 lineages. A phage dose effect observed across assay. Phages exhibited high MRSA. Bacteriophages represent promising anti-infective strategy ophthalmology could explored improved therapy infections.

Language: Английский

In Vitro Activity of Bacteriophages Against Ocular Methicillin-resistant S. aureus Isolates Collected in the US DOI Creative Commons
C.M. André, Mathieu Médina, Camille Kolenda

et al.

Ophthalmology and Therapy, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Methicillin-resistant Staphylococcus aureus (MRSA) is a leading cause of sight-threatening infections in the US. These strains pose significant challenge managing ocular infections, as they frequently exhibit resistance to first-line empirical antibiotics. To assess potential bacteriophages innovative topical therapies for treatment recalcitrant we evaluated vitro antimicrobial activity set anti-S. phages against collection MRSA clinical isolates collected The host range six (V4SA2, V1SA9, V1SA12, V1SA19, V1SA20 and V1SA22) was assessed using spot assay on panel 50 multidrug-resistant (MDR) selected be representative clones circulating Subsequently, liquid culture-based performed three most active different multiplicity infection (MOI 10–2, 1 or 100 phages/bacteria). In total, 90.0% bacterial were susceptible at least one phages. showed that V1SA22 had broadest spectrum, being 86%, 84% 82% isolates, respectively, including MDR-MRSA CC5 community-associated CC8 lineages. A phage dose effect observed across assay. Phages exhibited high MRSA. Bacteriophages represent promising anti-infective strategy ophthalmology could explored improved therapy infections.

Language: Английский

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