Serine Hydroxymethyltransferase Modulates Midgut Physiology in Aedes aegypti Through miRNA Regulation: Insights from Small RNA Sequencing and Gene Expression Analysis DOI Creative Commons
Qian Pu,

Yujiao Han,

Zhen Su

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(5), P. 644 - 644

Published: April 30, 2025

Aedes aegypti mosquitoes are critical vectors of arboviruses, responsible for transmitting pathogens that pose significant public health challenges. Serine hydroxymethyltransferase (SHMT), a key enzyme in one-carbon metabolism, plays vital role various biological processes, including DNA synthesis, energy and cell proliferation. Although SHMT is expressed at low levels the midgut aegypti, its silencing has been shown to inhibit blood meal digestion. The precise mechanisms by which regulates physiology remain poorly understood. In this study, we employed small RNA sequencing quantitative PCR identify differentially miRNAs (DEMs) following downregulation. We focused on subset DEMs—miR-2940-5p, miR-2940-3p, miR-2941, miR-306-5p—to explore their potential functions. To further elucidate molecular underlying miRNA response downregulation, analyzed expression genes involved biogenesis pathway. Our results demonstrated several enzymes, Drosha, Dicer1, AGO1, exhibited changes upon silencing. This study provides new insights into through influences functions nutritional metabolism mosquito midgut. By linking activity regulation, our findings highlight pathway modulates physiology, offering foundation future research biology vector control strategies.

Language: Английский

Serine Hydroxymethyltransferase Modulates Midgut Physiology in Aedes aegypti Through miRNA Regulation: Insights from Small RNA Sequencing and Gene Expression Analysis DOI Creative Commons
Qian Pu,

Yujiao Han,

Zhen Su

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(5), P. 644 - 644

Published: April 30, 2025

Aedes aegypti mosquitoes are critical vectors of arboviruses, responsible for transmitting pathogens that pose significant public health challenges. Serine hydroxymethyltransferase (SHMT), a key enzyme in one-carbon metabolism, plays vital role various biological processes, including DNA synthesis, energy and cell proliferation. Although SHMT is expressed at low levels the midgut aegypti, its silencing has been shown to inhibit blood meal digestion. The precise mechanisms by which regulates physiology remain poorly understood. In this study, we employed small RNA sequencing quantitative PCR identify differentially miRNAs (DEMs) following downregulation. We focused on subset DEMs—miR-2940-5p, miR-2940-3p, miR-2941, miR-306-5p—to explore their potential functions. To further elucidate molecular underlying miRNA response downregulation, analyzed expression genes involved biogenesis pathway. Our results demonstrated several enzymes, Drosha, Dicer1, AGO1, exhibited changes upon silencing. This study provides new insights into through influences functions nutritional metabolism mosquito midgut. By linking activity regulation, our findings highlight pathway modulates physiology, offering foundation future research biology vector control strategies.

Language: Английский

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