Gallium Resistance in Staphylococcus aureus: Polymorphisms and Morphology Impacting Growth in Metals, Antibiotics and Polyfluorinated Compounds DOI Creative Commons
Akamu J. Ewunkem,

Felicia Simpson,

Thomas L Holland

et al.

Applied Microbiology, Journal Year: 2025, Volume and Issue: 5(1), P. 32 - 32

Published: March 20, 2025

Background and Objectives: The imminent threat of antibiotic resistance has spurred studies nonconventional antimicrobial approaches. Gallium utilization is a promising emerging approach to treating variety resistant bacteria using “Trojan horse” strategies disrupt iron-dependent processes biofilms. This study utilized experimental evolution test the evolvability gallium in Staphylococcus aureus traits potentially correlated with metals, antibiotics polyfluorinated compounds, as well its genomics foundations. Methods: Whole-genome sequencing was reveal functional networks mutations associated resistance. Additionally, scanning electron microscopy (SEM) observation visualize distinct morphological changes on surface gallium-resistant populations compare control populations. Results: As demonstrated by these studies, S. evolved after 20 days selection. Furthermore, displayed heavy metals compounds. In contrast, were very sensitive antibiotics. analysis revealed significant polymorphisms (III)-resistant for example, staphyloferrinA export MFS transporter/D ornithine citrate ligase (sfaA/sfaD), teichoic acid D Ala esterase (fmtA), DUF3169 family protein (KQ76_RS01520) adenine phosphoribosyltransferase (KQ76_RS08360), while ABC transporter permease subunit (pstC) acyltransferase (KQ76_RS04365) unique directly affected cells’ morphology. SEM images showed external ultrastructural gallium-selected bacterial cells compared cells. Conclusions: Our confirmed that an can have health environmental implications.

Language: Английский

Gallium Resistance in Staphylococcus aureus: Polymorphisms and Morphology Impacting Growth in Metals, Antibiotics and Polyfluorinated Compounds DOI Creative Commons
Akamu J. Ewunkem,

Felicia Simpson,

Thomas L Holland

et al.

Applied Microbiology, Journal Year: 2025, Volume and Issue: 5(1), P. 32 - 32

Published: March 20, 2025

Background and Objectives: The imminent threat of antibiotic resistance has spurred studies nonconventional antimicrobial approaches. Gallium utilization is a promising emerging approach to treating variety resistant bacteria using “Trojan horse” strategies disrupt iron-dependent processes biofilms. This study utilized experimental evolution test the evolvability gallium in Staphylococcus aureus traits potentially correlated with metals, antibiotics polyfluorinated compounds, as well its genomics foundations. Methods: Whole-genome sequencing was reveal functional networks mutations associated resistance. Additionally, scanning electron microscopy (SEM) observation visualize distinct morphological changes on surface gallium-resistant populations compare control populations. Results: As demonstrated by these studies, S. evolved after 20 days selection. Furthermore, displayed heavy metals compounds. In contrast, were very sensitive antibiotics. analysis revealed significant polymorphisms (III)-resistant for example, staphyloferrinA export MFS transporter/D ornithine citrate ligase (sfaA/sfaD), teichoic acid D Ala esterase (fmtA), DUF3169 family protein (KQ76_RS01520) adenine phosphoribosyltransferase (KQ76_RS08360), while ABC transporter permease subunit (pstC) acyltransferase (KQ76_RS04365) unique directly affected cells’ morphology. SEM images showed external ultrastructural gallium-selected bacterial cells compared cells. Conclusions: Our confirmed that an can have health environmental implications.

Language: Английский

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