Polymicrobial urine cultures: reconciling contamination with the urobiome while recognizing the pathogens DOI Creative Commons
Robert B. Moreland, Linda Brubaker, Alan J. Wolfe

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: May 19, 2025

Polymicrobial or mixed urine cultures of more than one predominant microbe confound clinical urinary tract infection diagnosis. The current College American Pathologists laboratory standard states that a sample cultured with two isolates >10,000 colony forming units/ml is to be considered contaminated. However, the presence bacteria in individuals without symptoms (referred as asymptomatic bacteriuria) common especially older people and pregnant individuals. Furthermore, discovery an indigenous microbiome (urobiome) healthy humans throughout life from shortly after birth death conflicts long-standing notion derived sterile filtered blood should above urethral sphincter. infections are not consistent Koch’s postulates single pathogen causal for disease. In this review, we will discuss standards contamination, how reconcile sterility existence urobiome, history polymicrobial infections, why re-examining practices essential practice medicine, improving quality life, potentially saving lives.

Language: Английский

Polymicrobial urine cultures: reconciling contamination with the urobiome while recognizing the pathogens DOI Creative Commons
Robert B. Moreland, Linda Brubaker, Alan J. Wolfe

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: May 19, 2025

Polymicrobial or mixed urine cultures of more than one predominant microbe confound clinical urinary tract infection diagnosis. The current College American Pathologists laboratory standard states that a sample cultured with two isolates >10,000 colony forming units/ml is to be considered contaminated. However, the presence bacteria in individuals without symptoms (referred as asymptomatic bacteriuria) common especially older people and pregnant individuals. Furthermore, discovery an indigenous microbiome (urobiome) healthy humans throughout life from shortly after birth death conflicts long-standing notion derived sterile filtered blood should above urethral sphincter. infections are not consistent Koch’s postulates single pathogen causal for disease. In this review, we will discuss standards contamination, how reconcile sterility existence urobiome, history polymicrobial infections, why re-examining practices essential practice medicine, improving quality life, potentially saving lives.

Language: Английский

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