Antonie van Leeuwenhoek, Journal Year: 2021, Volume and Issue: 115(1), P. 125 - 139
Published: Nov. 29, 2021
Language: Английский
Scientific Reports, Journal Year: 2021, Volume and Issue: 11(1)
Published: June 21, 2021
Insects tend to live within well-defined habitats, and at smaller scales can have distinct microhabitat preferences. These preferences are important, but often overlooked, in applications of the sterile insect technique. Different wild insects may reflect differences environmental tolerance lead spatial separation field, both which reduce control program efficiency. In this study, we compared diurnal distributions mass-reared (fertile sterile) Queensland fruit flies, Bactrocera tryoni (Froggatt) (Diptera: Tephritidae). Flies were individually tagged released into field cages containing citrus trees. We recorded their locations canopies (height from ground, distance canopy center), behavior (resting, grooming, walking, feeding), abiotic conditions on occupied leaves (temperature, humidity, light intensity) throughout day. all groups moved lower when temperature intensity high, humidity was low; regions provided shelter these conditions. Fertile flies sexes generally than flies. fed top sides that canopy, suggesting food sources locations. Our observations suggest B. occupy different tree canopies, could indicate tolerances extremes result assessed a landscape scale.
Language: Английский
Citations
11
Insects, Journal Year: 2024, Volume and Issue: 15(9), P. 727 - 727
Published: Sept. 21, 2024
The predatory stink bug, Picromerus lewisi (Hemiptera: Pentatomidae), is an important and valuable natural enemy of insect pests in their ecosystems. While insects are known to harbor symbiotic microorganisms, these microbial symbionts play a crucial role various aspects the host’s biology, there paucity knowledge regarding microbiota present venom glands P. lewisi. This study investigated adult bugs using both traditional vitro isolation cultural methods, as well Illumina high-throughput sequencing technology. Additionally, carbon metabolism gland’s microorganisms was analyzed Biolog ECO metabolic phenotyping results showed 10 different culturable bacteria where dominant ones were Enterococcus spp. Lactococcus lactis. With sequencing, main bacterial phyla community Proteobacteria (78.1%) Firmicutes (20.3%), with genera being Wolbachia, Enterococcus, Serratia, Lactococcus. At fungal level, Ascomycota accounted for largest proportion (64.1%), followed by Basidiomycota (27.6%), Vishniacozyma, Cladosporium, Papiliotrema, Penicillium, Fusarium, Aspergillus most highly represented genera. structure exhibited high species richness diversity, along strong 22 sources. Functional prediction indicated that primary function venom-gland metabolism. eco-functional groups included undefined saprotroph, parasite–undefined unassigned, endophyte–plant pathogen, plant pathogen–soil saprotroph–wood animal pathogen–endophyte–plant pathogen–wood pathogen–endophyte–epiphyte–plant pathogen–undefined saprotroph. These provide comprehensive characterization demonstrate stability (over one week) within glands. represents first report on composition from captive-reared individuals. insights gained this invaluable future investigations into lewisi’s development possible interactions between some Lepidopteran pests.
Language: Английский
Citations
1
Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: 91(1)
Published: Dec. 20, 2024
Microbiome composition and function often change throughout a host's life cycle, reflecting shifts in the ecological niche of host. The mechanisms that establish these relationships are therefore important dimensions host ecology evolution; yet, their nature remains poorly understood. Here, we sought to investigate microbial communities associated with complex cycle dung beetle Onthophagus taurus relative contributions stage, sex, environment determining microbiome assembly. We find O. plays diverse microbiota undergo drastic community development, influenced by environmental microbiota, and, lesser degree, sex. Contrary predictions, found egg pupal stages-despite absence digestive tract or defined microbe-storing organs-do not constrain maintenance, while host-constructed environments, such as maternally derived fecal pellet chamber constructed late larvae, may still serve complementary refugia for select taxa. Lastly, identify small putative core likely shape development fitness. Our results provide insights into employed solitary organisms assemble, maintain, adjust beneficial confront life-stage-specific needs challenges. As influence symbionts on ecology, evolution, has become more apparent so importance understanding how hosts facilitate reliable maintenance interactions symbionts. A growing body work thus begun behaviors physiological underpinning selective colonization across range Yet, cycles, holometabolous insects, maintain key This is particularly interesting considering transformations both internal external morphology, diet environment, hallmark metamorphosis. investigates dynamic changes environments bull-headed beetle, taurus, useful model modulate development.
Language: Английский
Citations
1
Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13
Published: March 10, 2022
The genus Anastrepha (Diptera Tephritidae) includes some of the most important fruit fly pests in Americas. Here, we studied gut bacterial community 3rd instar larvae fraterculus sp. 1 through Next Generation Sequencing (lllumina) V3-V4 hypervariable region within 16S rRNA gene. Gut communities were compared between host species (guava and peach), geographical origins (Concordia Horco Molle Argentina) representing distinct ecological scenarios. In addition, explored effect spatial scale by comparing samples collected from different trees each geographic origin species. We also addressed size on diversity. was affected both origin. At smaller scales, profile differed among same location at least one host-location combination. There no larval bacteriome. Operational Taxonomic Units (OTUs) assigned to Wolbachia, Tatumella Enterobacter identified all examined, which suggest potential, non-transient symbioses. Better knowledge bacteriome contributes valuable information develop sustainable control strategies against A. targeting key symbionts as Achilles' heel this pest.
Language: Английский
Citations
6
Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 12(1)
Published: Dec. 14, 2023
ABSTRACT Ambrosia gall midges (AGMs) are mostly host plant-specific. In their galls, they harbor fungal symbionts on which feed. Therefore, represent unique steps in the evolution of gall-forming Cecidomyiidae (Diptera). Gall-associated fungi have been studied predominantly by cultivations, and potential larval endosymbionts completely neglected. Using ITS2 rRNA metabarcoding, we characterized mycobiomes individual compartments (gall surface, interior, larva) six species from two phylogenetically separated tribes (Asphondyliini Lasiopterini). Compared to surface larvae harbored significantly higher richness taxonomic diversity, a larger pool indicator taxa. Larval mycobiome composition was more species-specific; however, genera Fusarium , Filobasidium Tilletiopsis Alternaria Aureobasidium were taxa shared among species. Overall, 29% that can play functional role (e.g., initiation development or selection mycelia composition). The interior assembled least stochastically, its species-specific, being dominated Botryosphaeria dothidea (except for Lasioptera arundinis ). ambrosia galls offers environment supports growth similar fungi, regardless plant phylogenetic distance between AGM tribes. Our study illustrates range microorganisms indicative compartments, but function, especially larvae, remains be solved. IMPORTANCE endophagous insect herbivores whose live enclosed within single entire period. They may exhibit phytomycetophagy, remarkable feeding mode involves consumption biomass cultivated symbionts. Thus, AGMs ideal model organisms studying specificity insects. However, compared other fungus-farming insects, insect–fungus mutualism has is first use DNA metabarcoding characterize complete system insects as profiled surfaces, nutritive mycelia, larvae. Interestingly, different although evolutionary separation studied. confirmed long-time hypothesized paradigm important association this fungus with AGMs.
Language: Английский
Citations
3
Animal Microbiome, Journal Year: 2021, Volume and Issue: 3(1)
Published: Oct. 3, 2021
Abstract Interactions between microorganisms associated with metazoan hosts are emerging as key features of symbiotic systems. Little is known about the role such interactions on maintenance host-microorganism association throughout host’s life cycle. We studied influence extracellular bacteria a wild isolate yeast Saccharomyces cerevisiae through metamorphosis fly Drosophila melanogaster reared in fruit. Yeasts maintained only when larvae were isolated from D. faeces. One these isolates, an Enterobacteriaceae, favoured during metamorphosis. Such bacterial host-yeast may have consequences for ecology and evolution insect-yeast-bacteria symbioses wild.
Language: Английский
Citations
7
Journal of Pest Science, Journal Year: 2021, Volume and Issue: 95(1), P. 291 - 301
Published: May 30, 2021
Language: Английский
Citations
6
Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown
Published: March 29, 2023
Abstract The Queensland fruit fly (Bactrocera tryoni) is a major polyphagous pest widespread in Australia and several Pacific Islands. Bacteria present on the host plant phyllosphere supply proteins, essential for egg development female sexual maturity. We investigated role of microbial volatile organic compounds (MVOCs) attracting virgin females. Four Enterobacteriaceae commonly found gut B. tryoni were cultured different media (artificial natural fruits), their MVOCs collected using two headspace absorbent materials (Tenax® filter solid phase micro-extraction fibre; SPME), allowing to test time pH variations. olfactory responses females bacterial assessed via electrophysiology behavioural assays. production was strongly influenced qualitatively by strain, type quantitatively varied with time. Phenol detected only from Citrobacter freundii (CF), while indole all bacteria except CF. Indole increased overtime inoculated kiwifruits. D-limonene Enterobacter cloacae Klebsiella oxytoca (KO) associated oranges kiwifruit. Tenax extracts KO invoked strongest antennal response attracted most. Among identified triggering an response, 2-nonanone both significantly behaviourally attractive whereas phenol, nonanal, isoamyl alcohol some pyrazines appeared repulsive. This study provides new foundation developing synthetic lure based volatiles replace food baits targeting specifically
Language: Английский
Citations
2
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2020, Volume and Issue: unknown
Published: June 1, 2020
Abstract Interactions between microbial symbionts of metazoan hosts are emerging as key features symbiotic systems. Little is known about the role such interactions on maintenance symbiosis through host’s life cycle. We studied influence bacteria yeast metamorphosis fly Drosophila melanogaster . To this end we mimicked development larvae in natural fruit. In absence was never found young adults. However, could maintain when were inoculated with isolated from D. faeces. Furthermore, an Enterobacteriaceae favoured transstadial maintenance. Because a critical symbiont flies, bacterial host-yeast association may have consequences for evolution insect-yeast-bacteria tripartite and their cooperation. Summary statement Bacterial metamorphosis, novel observation that interactions.
Language: Английский
Citations
4
Journal of Fungi, Journal Year: 2021, Volume and Issue: 7(10), P. 845 - 845
Published: Oct. 9, 2021
Information about the fungal composition of bee bread, and fermentation processes to which fungi contribute significantly, is rather scarce or fragmentary. In this study, we performed an NGS-based metagenomics snapshot picture study bread in four locations Bulgaria during most active honeybee foraging period at end June 2020. The sampling were chosen differ significantly climatic conditions, landscape, anthropogenic pressure, Illumina 2 × 250 paired-end reads platform was used for amplicon ITS2 region. We found that some already reported canonical beneficial core species present within studied samples. However, genera such as Monilinia, Sclerotinia, Golovinomyces, Toxicocladosporium, Pseudopithomyces, Podosphaera Septoriella first time among dominant a related product. Anthropogenic pressure negatively influences two different ways—urban/industrial affects presence pathogenic species, while agricultural reflected decrease ratio fungi.
Language: Английский
Citations
4