Rice protein concentrate as a fish meal substitute in Oreochromis niloticus: Effects on immune response, intestinal cytokines, Aeromonas veronii resistance, and gut microbiota composition

Fish & Shellfish Immunology, Journal Year: 2022, Volume and Issue: 126, P. 237 - 250

Published: May 30, 2022

The potential of rice protein concentrate (RPC) to substitute fishmeal (FM) in the diet Oreochromis niloticus was assessed a five-month-long feeding trial. Fishmeal replaced by RPC at rates 0% (control), 25%, 50%, and 75% (RPC0, RPC25, RPC50, RPC75, respectively). RPC25 had no significant effect on antioxidant capacity (total capacity; superoxide dismutase, catalase, glutathione peroxidase activities) immune indices (lysozyme, nitric oxide, antiprotease, bactericidal after one, two, five months feeding, while values for these parameters were significantly lower RPC75 group compared those RPC0 group. showed higher mRNA levels intestinal cytokines IL-1β, IL-10β, TGF-β, TNF-α than control In fish affected Aeromonas veronii, highest cumulative mortality recorded group, followed groups. Gut microbiome analyses reduction microbial diversity response addition RPC, regardless content, composition community samples differed from that control. RPC-enriched diets resulted relative abundances Bacteroidetes Fusobacteria gut fish. summary, can be used replace up 25% FM O. niloticus, improving capacity, immunocompetence, disease resistance

Language: Английский

---

Characterization of the core gut microbiota of Nile tilapia (Oreochromis niloticus): indication of a putative novel Cetobacterium species and analysis of its potential function on nutrition

Archives of Microbiology, Journal Year: 2022, Volume and Issue: 204(12)

Published: Nov. 3, 2022

Language: Английский

---

Effect of Bacillus Probiotics on the Immunological Responses of Nile Tilapia (Oreochromis niloticus): A Review

Fishes, Journal Year: 2023, Volume and Issue: 8(7), P. 366 - 366

Published: July 13, 2023

The use of antibiotics and therapeutics for Nile tilapia has increased along with its production intensification. Probiotics have been studied as an alternative to the excessive in fish diets. Studies demonstrated that dietary Bacillus probiotic inclusion promotes faster growth disease resistance farmed species. Despite advances research, there is still uncertainty about mechanisms controlling immune response fish. In this review, we summarize discuss recent research on responses probiotics. Through immunological information including phagocytosis, lysozyme activity, respiratory antioxidant proteins, complement immune-related gene expression, researchers hope develop effective strategies successfully using probiotics Oreochromis niloticus farming.

Language: Английский

---

Gut Immune System and the Implications of Oral-Administered Immunoprophylaxis in Finfish Aquaculture

Frontiers in Immunology, Journal Year: 2021, Volume and Issue: 12

Published: Dec. 16, 2021

The gastrointestinal immune system plays an important role in homeostasis regulation. It regulates the symbiotic host-microbiome interactions by training and developing host’s innate adaptive immunity. This interaction a vital host defence mechanisms at same time, balancing endogenous perturbations of homeostasis. fish is armed with intricate diffused gut-associated lymphoid tissues (GALTs) that establish tolerance toward enormous commensal gut microbiome while preserving responses against intrusion enteric pathogens. A comprehensive understanding intestinal prerequisite for oral vaccine immunostimulants aquaculture, particularly cultured species. In this review, we outline remarkable features immunity essential components tissue. mechanistic principles underlying antigen absorption uptake through epithelial, subsequent activation series molecular events are reviewed. emphasis on significance administration immunoprophylactics, different potential adjuvants circumvent tolerance. Comprehension pivotal effective vaccines can be delivered orally, which less labour-intensive could improve health facilitate disease management aquaculture industry.

Language: Английский

---

The Impact of Sampling Season and Catching Site (Wild and Aquaculture) on Gut Microbiota Composition and Diversity of Nile Tilapia (Oreochromis niloticus)

Biology, Journal Year: 2021, Volume and Issue: 10(3), P. 180 - 180

Published: March 1, 2021

The gut microbiota of fishes is known to play an essential role in diverse aspects host biology. fish affected by various environmental parameters, including temperature changes, salinity and diet. Studies effect environment on enables have a further understanding what comprises healthy under different conditions. However, there insufficient regarding the effects sampling season catching site (wild aquaculture) Nile tilapia. This study characterised microbial composition diversity from samples collected Lake Tana Bahir Dar aquaculture facility centre using 16S rDNA Illumina MiSeq platform sequencing. Firmicutes Fusobacteria were most dominant phyla samples, while Proteobacteria was samples. results differential abundance testing clearly indicated significant differences for Firmicutes, Fusobacteria, Bacteroidetes Cyanobacteria across months. Proteobacteria, Chloroflexi, significantly enriched comparison centre. Significant observed months between wild captive alpha showed that (captive) had higher than tilapia Tana. core all used our comprised Fusobacteria. impact bacterial communities associated with Overall, this first Ethiopia. Future work recommended precisely explain causes these changes large representative lakes farms.

Language: Английский

---

Taxonomic and Functional Characteristics of the Gill and Gastrointestinal Microbiota and Its Correlation with Intestinal Metabolites in NEW GIFT Strain of Farmed Adult Nile Tilapia (Oreochromis niloticus)

Microorganisms, Journal Year: 2021, Volume and Issue: 9(3), P. 617 - 617

Published: March 17, 2021

The gill and gastrointestinal tract are primary entry routes for pathogens. symbiotic microbiota essential to the health, nutrition disease of fish. Though intestinal Nile tilapia (Oreochromis niloticus) has been extensively studied, information on mucosa-associated this species, especially microbiota, is lacking. This study aimed characterize mucosa- digesta-associated as well metabolite profiles in New Genetically Improved Farmed Tilapia (NEW GIFT) strain farmed adult by high-throughput sequencing gas chromatography/mass spectrometry metabolomics. diversity, structure, composition, predicted function were significantly different across regions sample types (Welch t-test; p < 0.05). By comparing linear discriminant analysis (LDA) effect size (LEfSe) revealed that Pelomonas, Ralstoniapickettii, Comamonadaceae, Staphylococcus enriched whereas many bacterial taxa including Chitinophagaceae, Cetobacterium, CandidatusCompetibacter, Methyloparacoccus, chloroplast (LDA score > 3.5). Furthermore, Undibacterium, Escherichia-Shigella, Paeniclostridium, Cetobacterium dominant contents mucosae, Sphingomonasaquatilis Roseomonasgilardii commonly found stomach mucosae. Phylogenetic Investigation Communities Reconstruction Unobserved States (PICRUSt2) predictive differed from (R = 0.8152, 0.0001). In addition, our results showed a significant interdependence between specific microbes metabolites. Notably, relative abundance values several potentially beneficial microbes, Crenothrix, positively correlated with most metabolites, some potential opportunistic pathogens, Acinetobacter, Mycobacterium, Aeromonas, Clostridiumsensustricto 1, negatively characteristics identified close correlation serve basis effective application targeted probiotics or prebiotics diet regulate health tilapia.

Language: Английский

---

The gut bacterial microbiome of Nile tilapia (Oreochromis niloticus) from lakes across an altitudinal gradient

BMC Microbiology, Journal Year: 2022, Volume and Issue: 22(1)

Published: April 4, 2022

Abstract Background Microorganisms inhabiting the gut play a significant role in supporting fundamental physiological processes of host, which contributes to their survival varied environments. Several studies have shown that altitude affects composition and diversity intestinal microbial communities terrestrial animals. However, little is known about impact on microbiota aquatic The current study examined variations Nile tilapia ( Oreochromis niloticus ) from four lakes along an altitudinal gradient Ethiopia by using 16S rDNA Illumina MiSeq high-throughput sequencing. Results results indicated low-altitude samples typically displayed greater alpha diversity. principal coordinate analysis (PCoA) showed differences across different lakes. Firmicutes was most abundant phylum Lake Awassa Chamo whereas Fusobacteriota dominant Hashengie Tana. ratio Bacteroidota high-altitude sample (Lake Hashengie, 2440 m) much higher than low population Chamo, 1235 m). We found relative abundances Actinobacteriota, Chloroflexi, Cyanobacteria, were negatively correlated with altitude, while positive association altitude. Despite variability abundance lakes, some shared bacterial detected. Conclusions In summary, this indirect influence microbiota. Altitude has potential modulate tilapia. Future work will be needed elucidate functional significance based geographical environment. Significance Our determined collected gradient. findings greatly extend baseline knowledge fish Ethiopian plays important species sustainable aquaculture activities conservation.

Language: Английский

---

Investigating the Effect of an Oxytetracycline Treatment on the Gut Microbiome and Antimicrobial Resistance Gene Dynamics in Nile Tilapia (Oreochromis niloticus)

Antibiotics, Journal Year: 2021, Volume and Issue: 10(10), P. 1213 - 1213

Published: Oct. 6, 2021

Antibiotics play a vital role in aquaculture where they are commonly used to treat bacterial diseases. However, the impact of antibiotic treatment on gut microbiome and development antimicrobial resistance Nile tilapia (Oreochromis niloticus) over time remains be fully understood. In this study, fish were fed single oxytetracycline (100 mg/kg/day) for eight days, followed by 14-day withdrawal period. Changes distal measured using 16S rRNA sequencing. addition, abundance genes was quantified real-time qPCR methods. Overall, community diversity structure resilient treatment. associated with an enrichment Plesiomonas, accompanied decline other bacteria taxa. Oxytetracycline increased proportion tetA tank biofilms treated group. Furthermore, along tetracycline strongly correlated number members, including Plesiomonas. The findings from study demonstrate that can exert selective pressures favour resistant populations, which may have long-term impacts health.

Language: Английский

---

Intestine and spleen microbiota composition in healthy and diseased tilapia

Animal Microbiome, Journal Year: 2022, Volume and Issue: 4(1)

Published: Aug. 13, 2022

Symbiotic bacteria within the gut microbiome of various organisms, including fish, provide host with several functions that improve immune system. Although spleen plays an important role in modulation responses, microbiota shaping system is unclear. Our study aimed at understanding relationship between fish health and composition intestine spleen. model organism was hybrid tilapia (Oreochromis aureus × Oreochromis niloticus). We sampled from healthy diseased adult determined their by sequencing 16S rRNA gene. Significant differences were found compared to as well intestines spleens same condition. The diversity significantly different well. In Cetobacterium most abundant genus while Mycoplasma Vibrio fish. Moreover, it seems there a co-infection interaction Aeromonas, which reflected While Vibrio, Aeromonas Streptococcus probable pathogens pathogen cultured remains uncertain. conclude strongly related condition

Language: Английский

---

Significant alterations of intestinal symbiotic microbiota induced by intraperitoneal vaccination mediate changes in intestinal metabolism of NEW Genetically Improved Farmed Tilapia (NEW GIFT, Oreochromis niloticus)

Microbiome, Journal Year: 2022, Volume and Issue: 10(1)

Published: Dec. 12, 2022

After millions of years coevolution, symbiotic microbiota has become an integral part the host and plays important role in immunity, metabolism, health. Vaccination, as effective means preventing infectious diseases, been playing a vital prevention control human animal diseases for decades. However, so far, minimal is known about effect vaccination on fish microbiota, especially mucosal its correlation with intestinal metabolism remains unclear.Here we reported inactivated bivalent Aeromonas hydrophila/Aeromonas veronii vaccine farmed adult Nile tilapia (Oreochromis niloticus) by 16S rRNA gene high-throughput sequencing gas chromatography-mass spectrometry metabolomics.Results showed that significantly changed structure, composition, predictive function but did not affect other sites including gill mucosae, stomach contents, mucosae. Moreover, reduced relative abundance values potential opportunistic pathogens such Aeromonas, Escherichia-Shigella, Acinetobacter Combined enhancement immune after vaccination, had protective against pathogen infection tilapia. In addition, metabolite differential analysis increased concentrations carbohydrate-related metabolites lactic acid, succinic gluconic acid decreased multiple lipid-related intestines. Vaccination affected tilapia, which was further verified microbiota. Furthermore, analyses most microorganisms were correlated confirming closely related to microbiota.In conclusion, this paper revealed microbial metabolic responses induced suggesting might mediate It expanded novel understanding mechanisms from perspectives, providing implications influence metabolism. Video Abstract.

Language: Английский

---