Risk assessment requires several bee species to address species-specific sensitivity to insecticides at field-realistic concentrations

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Dec. 18, 2023

Abstract In the European registration process, pesticides are currently mainly tested on honey bee. Since sensitivity data for other bee species lacking majority of xenobiotics, it is unclear if and to which extent this model can adequately serve as surrogate all wild bees. Here, we investigated effects field-realistic contact exposure a pyrethroid insecticide, containing lambda-cyhalothrin, seven ( Andrena vaga , Bombus terrestris Colletes cunicularius Osmia bicornis cornuta, Megachile rotundata, Apis mellifera ) with different life history characteristics in series laboratory trials over two years. Our results showed significant species-specific responses pesticide at application rate (i.e., 7.5 g a.s./ha). Species did not group into distinct classes high low mortality. Bumble mason survival was least affected by M. rotundata most individuals dead 48 h after application. medium mortality compared species. Most sublethal effects, i.e. behavioral abnormalities, were observed within first hours some solitary species, example O. A. vaga, higher percentage performed abnormal behavior longer until end observation period. While individual weight explained patterns, differences likely linked additional ecological, phylogenetic or toxicogenomic parameters well. support idea that be substitute species’ may justify usage safety factors. To cover more sensitive larger set should considered risk assessment.

Language: Английский

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Topical exposure of honey bee queens to heat-inactivated Israeli acute paralysis virus does not protect their offspring against active infection

Apidologie, Journal Year: 2025, Volume and Issue: 56(1)

Published: Jan. 14, 2025

Language: Английский

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Gut Microbiome Diversity in European Honeybees (Apis mellifera L.) from La Union, Northern Luzon, Philippines

Insects, Journal Year: 2025, Volume and Issue: 16(2), P. 112 - 112

Published: Jan. 23, 2025

Insects often rely on symbiotic bacteria and fungi for various physiological processes, developmental stages, defenses against parasites diseases. Despite their significance, the associations between bacterial fungal symbionts in Apis mellifera are not well studied, particularly Philippines. In this study, we collected A. from two different sites Municipality of Bacnotan, La Union, A gut microbiome analysis was conducted using next-generation sequencing with Illumina MiSeq platform. Bacterial community compositions were assessed 16S rRNA ITS gene sequences, respectively. Our findings confirm that adult worker bees locations possess distinct but comparably proportioned microbiomes. Key symbionts, including Lactobacillus, Bombilactobacillus, Bifidobacterium, Gilliamella, Snodgrassella, Frischella, identified. The dominated by yeasts Zygosaccharomyces Priceomyces. Using ENZYME nomenclature database PICRUSt2 software version 2.5.2, a predicted functional enzyme revealed presence β-glucosidase, catalase, glucose-6-phosphate dehydrogenase, glutathione transferase, superoxide dismutase, which involved host defense, carbohydrate metabolism, energy support. Additionally, identified notable enzymes, acetyl-CoA carboxylase AMPs nucleosidase. Interestingly, key bee observed to have negative correlation other microbiota. These results provide detailed characterization microbiota associated Philippines lay foundation further metagenomic studies microbiomes native or indigenous species region.

Language: Английский

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The gut microbiome differs between hygiene-performing and non-hygiene-performing worker honey bees

Insectes Sociaux, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

Language: Английский

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Gut microbiota analysis of healthy and sacbrood virus-infected <em> Apis mellifera </em> reveals potential probiotic bacteria for honeybee health and disease resistance

ACADEMIA JOURNAL OF BIOLOGY, Journal Year: 2025, Volume and Issue: 47(1), P. 33 - 43

Published: March 27, 2025

This study aimed to determine the gut microbiota composition of adult Apis mellifera honeybees from bee farms in Bac Giang province, including both healthy colonies and those infected with Sacbrood virus (SBV). The SBV-infected bees was assessed using next-generation sequencing (NGS) V3-V4 region 16S rRNA gene on Illumina MiSeq system. As a result, NGS analysis identified 1,659 operational taxonomic units (OTUs) coverage 99% an average read length 430 bp. results revealed that harbored four microbial phyla: Proteobacteria (48.44%), Firmicutes (38.65%), Actinobacteria (1.57%), Bacteria_uc (10.95%). In contrast, group consisted three (40.61%), (45.55%), (13.37%). species showed shared common core bacterial species. However, Bifidobacterium_uc Commensalibacter AY370188_s were more prevalent significantly reduced bees. Conversely, Fructobacillus fructosus Lactobacillus kunkeei found exclusively These lactic acid bacteria (LAB) have been shown inhibit growth pathogenic bacteria. Our findings provide valuable scientific foundation for developing biological products improve honeybee health disease resistance.

Language: Английский

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