Progress in the use of DNA barcodes in the identification and classification of medicinal plants

Ecotoxicology and Environmental Safety, Journal Year: 2020, Volume and Issue: 208, P. 111691 - 111691

Published: Nov. 28, 2020

DNA barcoding is an emerging molecular identification and classification technology that has been applied to medicinal plants since 2008. The application of this technique greatly ensured the safety effectiveness materials. In paper, we review some related technologies over past 10 years with respect improving our knowledge plant authentication. From single locus-based barcodes combined markers genome-scale levels, contribute more genetic information. At same time, other technologies, such as high-resolution melting (HRM), have barcoding. With development next-generation sequencing (NGS), metabarcoding also shown identify species in mixed samples successfully. As a widely used effective tool, will become useful time field plants.

Language: Английский

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The Complete Chloroplast Genome Sequence of Tree of Heaven (Ailanthus altissima (Mill.) (Sapindales: Simaroubaceae), an Important Pantropical Tree

International Journal of Molecular Sciences, Journal Year: 2018, Volume and Issue: 19(4), P. 929 - 929

Published: March 21, 2018

Ailanthus altissima (Mill.) Swingle (Simaroubaceae) is a deciduous tree widely distributed throughout temperate regions in China, hence suitable for genetic diversity and evolutionary studies. Previous studies A. have mainly focused on its biological activities, structure. However, until now there no published report regarding genome of this plant species or Simaroubaceae family. Therefore, paper, we first characterized complete chloroplast sequence. The heaven was found to be circular molecule 160,815 base pairs (bp) size possess quadripartite contains 113 unique genes which 79 30 are protein coding transfer RNA (tRNA) respectively also 4 ribosomal (rRNA) with overall GC content 37.6%. Microsatellite marker detection identified A/T mononucleotides as majority SSRs all the seven analyzed genomes. Repeat analyses Sapindales revealed total 49 repeats altissima, Rhus chinensis, Dodonaea viscosa, Leitneria floridana, while Azadirachta indica, Boswellia sacra, Citrus aurantiifolia had 48 repeats. phylogenetic analysis using that sister floridana suggested Rutaceae information reported here could further applied evolution invasion, population genetics, molecular

Language: Английский

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Comparative analyses of chloroplast genomes from 22 Lythraceae species: inferences for phylogenetic relationships and genome evolution within Myrtales

BMC Plant Biology, Journal Year: 2019, Volume and Issue: 19(1)

Published: June 26, 2019

Lythraceae belongs to the order Myrtales, which is part of Archichlamydeae. The family has 31 genera containing approximately 620 species herbs, shrubs and trees. Of these genera, five large each possess 35 or more species. They are Lythrum, with 35; Rotala, 45; Nesaea, 50; Lagerstroemia, 56; Cuphea, 275

Language: Английский

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Comparative Analysis of Chloroplast Genome of Meconopsis (Papaveraceae) Provides Insights into Their Genomic Evolution and Adaptation to High Elevation

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(4), P. 2193 - 2193

Published: Feb. 12, 2024

The Meconopsis species are widely distributed in the Qinghai-Tibet Plateau, Himalayas, and Hengduan Mountains China, have high medicinal ornamental value. diversity of plant morphology this genus poses significant challenges for identification, given their propensity highland dwelling, which makes it a question worth exploring how they cope with harsh surroundings. In study, we recently generated chloroplast (cp) genomes two species, paniculata (M. paniculata) M. pinnatifolia, compared them those ten cp to comprehend genomic features, phylogenetic relationships, what part might play plateau adaptation. These shared great deal similarities terms genome size, structure, gene content, GC codon usage patterns. were between 151,864 bp 154,997 length, contain 133 predictive genes. Through sequence divergence analysis, identified three highly variable regions (trnD-psbD, ccsA-ndhD, ycf1 genes), could be used as potential markers or DNA barcodes analysis. Between 22 38 SSRs some long repeat sequences from 12 species. Our analysis confirmed that clustered into monophyletic clade Papaveraceae, corroborated intrageneric relationships. results indicated pinnatifolia sister tree. addition, atpA ycf2 genes positively selected high-altitude functions these involved adaptation extreme environment cold low CO2 concentration conditions at plateau.

Language: Английский

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Complete Chloroplast Genome Sequences of Kaempferia Galanga and Kaempferia Elegans: Molecular Structures and Comparative Analysis

Molecules, Journal Year: 2019, Volume and Issue: 24(3), P. 474 - 474

Published: Jan. 29, 2019

Kaempferia galanga and elegans, which belong to the genus family Zingiberaceae, are used as valuable herbal medicine ornamental plants, respectively. The chloroplast genomes have been for molecular markers, species identification phylogenetic studies. In this study, complete genome sequences of K. elegans reported. Results show that is 163,811 bp long, having a quadripartite structure with large single copy (LSC) 88,405 small (SSC) 15,812 separated by inverted repeats (IRs) 29,797 bp. Similarly, 163,555 in IRs 29,773 length separates 88,020 LSC 15,989 SSC. A total 111 genes 113 comprised 79 protein-coding 4 ribosomal RNA (rRNA) genes, well 28 30 transfer (tRNA) gene order, GC content orientation two exhibited high similarity. location distribution simple sequence (SSRs) long repeat were determined. Eight highly variable regions between identified 643 mutation events, including 536 single-nucleotide polymorphisms (SNPs) 107 insertion/deletions (indels), accurately located. Sequence divergences whole calculated among related Zingiberaceae species. analysis based on SNPs eleven strongly supported formed cluster within Zingiberaceae. This study unique characteristics entire contribute our understanding DNA evolution It provides information Kaempferia.

Language: Английский

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Comparative and Phylogenetic Analyses of Ginger (Zingiber officinale) in the Family Zingiberaceae Based on the Complete Chloroplast Genome

Plants, Journal Year: 2019, Volume and Issue: 8(8), P. 283 - 283

Published: Aug. 12, 2019

Zingiberofficinale, commonly known as ginger, is an important plant of the family Zingiberaceae and widely used herbal medicine condiment. The lack chloroplast genomic information hinders molecular research phylogenetic analysis on ginger. We introduced complete genome Z. officinale identified its position in Zingiberaceae. 162,621 bp with a four-part circular structure 36.1% GC content. All 113 unique genes were annotated. A total 78 simple sequence repeats (SSRs) 42 long repeat sequences, which are potential areas for species authentication, found. Comparative revealed some highly variable regions, including rps16-trnQ-UUG, atpH-atpI, trnT-UGU-trnL-UAA, ycf1, psaC-ndhE. Moreover, small single-copy (SSC) region was most all four shared indicating that it may be undergoing rapid nucleotide substitution Phylogenetic based available chloroplasts Zingiberales National Center Biotechnology Information indicated Zingiber sister branch to Kaempferia species. availability provided invaluable data species-level authentication can thus benefit further investigations

Language: Английский

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Complete chloroplast genome sequence of Barleria prionitis, comparative chloroplast genomics and phylogenetic relationships among Acanthoideae

BMC Genomics, Journal Year: 2020, Volume and Issue: 21(1)

Published: June 6, 2020

Abstract Background The plastome of medicinal and endangered species in Kingdom Saudi Arabia, Barleria prionitis was sequenced. compared with that seven Acanthoideae order to describe the plastome, spot microsatellite, assess dissimilarities within sampled plastomes infer their phylogenetic relationships. Results B. 152,217 bp length Guanine-Cytosine Adenine-Thymine content 38.3 61.7% respectively. It is circular quadripartite structure constitute a large single copy (LSC, 83, 772 bp), small (SSC, 17, 803 bp) pair inverted repeat (IRa IRb 25, 321 each). 131 genes were identified out which 113 are unique 18 repeated IR region. genome consists 4 rRNA, 30 tRNA 80 protein-coding genes. analysis long showed all types repeats present palindromic has highest frequency. A total number 98 SSR also mostly mononucleotide located at non coding regions. Comparative genomic among revealed more conserved than In addition high variation observed intergenic spacer region genes, ycf1 ndhF border junction plastome. sequence divergence protein indicates following undergo positive selection ( atpF, petD, psbZ, rpl20, petB, rpl16 , rps16, rpoC, rps7, rpl32 ycf3 ). Phylogenetic indicated sister relationship between Ruellieae Justcieae. addition, Justicia Ruellia paraphyletic, suggesting Justiceae, Ruellieae, Andrographideae Barlerieae should be treated as tribes. Conclusions This study sequenced assembled first taxon reported basics resources for evolutionary studies tools core Acanthaceae.

Language: Английский

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The Complete Plastid Genome of Magnolia zenii and Genetic Comparison to Magnoliaceae species

Molecules, Journal Year: 2019, Volume and Issue: 24(2), P. 261 - 261

Published: Jan. 11, 2019

Magnolia zenii is a critically endangered species known from only 18 trees that survive on Baohua Mountain in Jiangsu province, China. Little information available regarding its molecular biology, with no genomic study performed M. until now. We determined the complete plastid genome of and identified microsatellites. Whole sequence alignment phylogenetic analysis using BI ML methods were also conducted. The plastome was 160,048 bp long 39.2% GC content included pair inverted repeats (IRs) 26,596 separated large single-copy (LSC) region 88,098 small (SSC) 18,757 bp. One hundred thirty genes identified, which 79 protein-coding genes, 37 transfer RNAs, eight ribosomal RNAs. Thirty seven simple (SSRs) identified. Comparative analyses structure data closely-related revealed five mutation hotspots, useful for future research. placed as sister to biondii strong support all analyses. Overall, this providing resources will be beneficial evolutionary reconstruction Magnoliaceae.

Language: Английский

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The Complete Chloroplast Genomes of Two Lespedeza Species: Insights into Codon Usage Bias, RNA Editing Sites, and Phylogenetic Relationships in Desmodieae (Fabaceae: Papilionoideae)

Plants, Journal Year: 2019, Volume and Issue: 9(1), P. 51 - 51

Published: Dec. 31, 2019

The genus Lespedeza (tribe: Desmodieae) consists of about 40 species that have high medicinal and economic value. However, in this genus, using morphological characters, the identification is quite complicated, which can be solved by analysis complete chloroplast genomes. As primary organelle genomes, genome sequences chloroplasts (cp) provide unique molecular information to study divergence species, RNA editing, phylogeny. Therefore, best our knowledge, for first time, we sequenced cp genomes two representative species: Lespedezadavurica Lespedezacuneata. both were found 149,010 bp length, exhibiting typical angiosperm structure containing four regions. showed similar conserved gene contents, order, orientations with a total GC content 35.0%. A 128 genes, including 83 protein-coding 37 tRNAs, eight rRNAs, identified from each genome. Unique features obtained performing repeats, sequence divergence, codon usage, predicting editing sites addition phylogenetic other key genera tribe Desmodieae. Using datasets, relationship among Deasmodieae was discovered, suggesting whole provided useful studies these species.

Language: Английский

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Papaver Plants: Current Insights on Phytochemical and Nutritional Composition Along with Biotechnological Applications

Oxidative Medicine and Cellular Longevity, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 23

Published: Feb. 3, 2022

The genus Papaver is highly esteemed in the pharmacy industry, culinary field, and as ornamental plants. These plants are also valued traditional medicine. Among all species, somniferum L. (opium poppy) most important species supplying phytochemicals for formulation of drugs, mainly alkaloids like morphine, codeine, rhoeadine, thebaine, papaverine. In addition, present other types phytochemicals, which altogether responsible its biological activities. Therefore, this review covers phytochemical composition plants, including alkaloids, phenolic compounds, essential oils. uses reviewed along with their pharmacological Moreover, safety aspects reported to provide a deep overview pharmacology potential genus. An updated search was carried out databases such Google Scholar, ScienceDirect, PubMed retrieve information. Overall, rich source different contains interesting anthocyanins, flavonols, characteristic indole derivatives nudicaulins. properties, numerous preclinical studies have been published about analgesic, anticancer, antimicrobial, antioxidant, antidiabetic activities Although it highlights significant impact treatment variety diseases conditions, future prospect, characterization works accompanying required clinical toxicology establish correlation between scientific knowledge.

Language: Английский

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