Nanomaterials for co‐immobilization of multiple enzymes

BMEMat, Journal Year: 2024, Volume and Issue: unknown

Published: March 12, 2024

Abstract In order to co‐immobilize multiple enzymes, a wide range of nanomaterials has been designed achieve synergistic enzyme activity and enhance catalytic efficiency. Nanomaterials, as carriers for co‐immobilization, possess various advantages such tunable morphology size, high specific surface area, abundant chemically active sites. They can significantly stability, activity, We overview the commonly used methods strategies co‐immobilization. This review further summarizes latest research advances in co‐immobilization applications over past 5 years. Meanwhile, challenges these well some potential future directions are also discussed.

Language: Английский

---

The looming threat of profenofos organophosphate and microbes in action for their sustainable degradation

Environmental Science and Pollution Research, Journal Year: 2024, Volume and Issue: 31(10), P. 14367 - 14387

Published: Jan. 30, 2024

Language: Английский

---

Immobilization of Enological Pectinase on Magnetic Sensitive Polyamide Microparticles for Wine Clarification

Foods, Journal Year: 2024, Volume and Issue: 13(3), P. 420 - 420

Published: Jan. 28, 2024

The use of free pectinases as clarification biocatalysts constitutes a well-established practice in the large-scale production various types wines. However, when form enzymes, recovery and reusability is difficult if not impossible. To address these limitations, present study focuses on noncovalent adsorption immobilization commercial pectinolytic preparation onto highly porous polyamide 6 (PA6) microparticles, both with without magnetic properties, prepared via activated anionic polymerization. two pectinase complexes resulting after underwent comparative activity kinetic studies, contrasting them enzyme preparation. In comparison enzyme, PA6-immobilized exhibited more than double specific toward pectin substrate. They displayed slightly higher affinity to substrate while acting faster catalysts that were resistant inhibition. Furthermore, immobilized applied process industrial rosé must, whereby they demonstrated accelerated performance compared enzyme. Moreover, offered potential for three consecutive cycles reuse, achieving complete must within relevant timeframes range 3-36 h. All results suggest application noncovalently upon PA6 microparticles.

Language: Английский

---

Fast, Multiple-Use Optical Biosensor for Point-of-Care Glucose Detection with Mobile Devices Based on Bienzyme Cascade Supported on Polyamide 6 Microparticles

Polymers, Journal Year: 2023, Volume and Issue: 15(13), P. 2802 - 2802

Published: June 24, 2023

Non-invasive glucose determination provides major advantages in health monitoring and protection. It enables widespread point-of-care testing, which is affordable, sensitive, specific, rapid equipment-free. This work reports on the development analytical performance of a colorimetric biosensor detecting human urine. Highly porous polyamide microparticles were synthesized as support for oxidase (GOx) horseradish peroxidase (HRP) dyad, was immobilized randomly or consecutively-first HRP then GOx. The latter system superior, GH@PA-C showed much higher activity than random system, it used to prepare biosensor, along with 3,3',5,5'-tetramethylbenzidine chromogen. When contact urine, displayed strict linear correlation between color difference concentration urine range 0.01-3.0 mM, established by CIELab image processing algorithm UV-VIS measurements. acted 20 s had detection limit 30.7 µM high operational at pH = 4-8 unchanged after 30 days storage. Its unique feature possibility multiple consecutive uses without serious deterioration recovery dispersion values. These characteristics can open way new routines non-invasive personal diabetes detection.

Language: Английский

---

Transforming cancer detection and treatment with nanoflowers

Medical Oncology, Journal Year: 2024, Volume and Issue: 41(11)

Published: Oct. 22, 2024

Language: Английский

---