The ABA/LANCL1-2 Hormone/Receptors System Controls ROS Production in Cardiomyocytes through ERRα

Biomedicines, Journal Year: 2024, Volume and Issue: 12(9), P. 2071 - 2071

Published: Sept. 11, 2024

Rat H9c2 cardiomyocytes overexpressing the abscisic acid (ABA) hormone receptors LANCL1 and LANCL2 have an increased mitochondrial proton gradient, respiration, vitality after hypoxia/reoxygenation. Our aim was to investigate role of ABA/LANCL1-2 system in ROS turnover cells. cells were retrovirally infected induce overexpression or silencing LANCL2, without with concomitant transcription factor ERRα. Enzymes involved radical production scavenging studied by qRT-PCR Western blot. The gradient measured specific fluorescent probes. ROS-generating enzymes decreased, ROS-scavenging increased, reduced LANCL1/2-overexpressing vs. control empty vector, while opposite occurred LANCL1/2-silenced knockdown ERRα abrogated all beneficial effects on LANCL1/2 Taken together, these results indicate that controls via ABA/LANCL emerges as a promising target improve cardiomyocyte function resilience oxidative stress.

Language: Английский

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Obesity-induced activation of NADPH oxidase 2 prolongs cardiac repolarization via inhibiting K+ currents

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0316701 - e0316701

Published: Dec. 31, 2024

Obesity is associated with abnormal repolarization manifested by QT interval prolongation, and oxidative stress an important link between obesity arrhythmias. However, the underlying electrophysiological molecular mechanisms remain unclear. The aim of this study to evaluate role in potassium current ventricular myocytes potential mechanism NADPH oxidase 2 (Nox2). We investigated effect Nox2 on cardiac without compromising its expression function other systems using mice conditional cardiac-specific deletions (knockout [KO]). Wild-type, KO, Flox littermate were randomized either control or high-fat diet (HFD) groups. Surface electrocardiograms recorded analyze vivo. Whole-cell patch-clamp techniques used phenotype isolated vitro. Western blotting was performed assess protein levels. Compared mice, HFD group had a prolonged QTc. consequences not attributed delayed rectifier K + inward-rectifier currents but reduced peak outward V fast transient currents. Downregulated 4.2 KChIP2, comprising functional I channel pore-forming (α) accessory (β) subunits, detected mice. Nox2-KO reversed amplitude. Our data demonstrate that mediates impaired QTc at whole organism level action duration cellular level, correlated Nox2. aspects phenomenon mediated repolarizing

Language: Английский

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Thymoquinone reverses homocysteine-induced endothelial dysfunction via inhibition of ER-stress induced oxidative stress pathway

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: May 30, 2023

Abstract Hyperhomocysteinemia has been linked to an increased risk of cardiovascular diseases. High levels homocysteine (Hcy) promote endoplasmic reticulum (ER) stress that can increase reactive oxygen species (ROS), leading endothelial dysfunction. Thymoquinone (TQ) is the major active ingredient in Nigella sativa seeds volatile oil and shown have a cardioprotective effect. However, no study evaluated effect TQ against Hcy-induced Thus, this aims investigate effects mechanisms reversing Isolated aorta from male Sprague-Dawley (SD) rats incubated with Hcy (500 µM) co-treated or without (0.1 µM, 1 10 µM), 20 µM TUDCA, 100 Apocynin mM Tempol organ bath vascular function. Additionally, human umbilical vein cells (HUVECs) were (10 mM) various concentrations (1 𝜇M), (100 TUDCA 𝜇M) H 2 O (0.25 evaluate cell viability by using phase contrast microscope dye exclusion assay. Involvement ER pathway, ROS NO bioavailability accessed via immunoassay fluorescent staining respectively. Molecular docking was performed binding affinity GRP78. Our results revealed impaired endothelium-dependant relaxation isolated induced apoptosis HUVECs. These reversed TQ, tempol apocynin. Treatment (10𝜇M) also reduced level, improved as well GRP78 NOX4 protein Result molecular showed could bind through hydrogen bond hydrophobic interaction amino acid at ATP pocket. Taken together, present suggest preserved function rat HUVECs inhibition stress-mediated eNOS uncoupling.

Language: Английский

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Research Progress on the Medicinal and Edible Polygala fallax Hemsl. (Polygalaceae) Plant

Horticulturae, Journal Year: 2023, Volume and Issue: 9(7), P. 737 - 737

Published: June 23, 2023

Polygala fallax Hemsl., a species belonging to the genus and family Polygalaceae, is primarily distributed in southern China. Wild P. Hemsl. (Polygalaceae) currently endangered, but its utilization value has not yet been thoroughly investigated. The present review summarizes morphological structural characteristics, wild resource distribution, chemical constituents pharmacological efficacy, artificial breeding cultivation techniques, of facilitate future studies on conservation use Hemsl plants. We conclude that (i) medicinal edible plant, also eco-economic ornamental value; (ii) roots are primary parts, which contain pharmacologically active compounds such as saponins, flavones, oligosaccharide polyesters, polysaccharides, sterols, organic acids, etc.; (iii) shows various efficacies including anti-inflammatory effects, antivirus antioxidative an immunity-boosting function, hepatic-protective hypolipidemic effect, blood-activating, stasis-eliminating antitumor, etc. More research applications needed, especially efforts identify effective constituents, quality control, clinical applications. resources should be enhanced, aboveground efficacy other flavones further explored.

Language: Английский

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