Doxorubicin Attenuates Free Fatty Acid-Induced Lipid Accumulation via Stimulation of p53 in HepG2 Cells DOI Creative Commons
Chawon Yun, Sou Hyun Kim, Doyoung Kwon

et al.

Biomolecules & Therapeutics, Journal Year: 2023, Volume and Issue: 32(1), P. 94 - 103

Published: Dec. 27, 2023

Non-alcoholic fatty liver disease (NAFLD) is characterized by excessive accumulation of fat in the liver, and there a global increase its incidence owing to changes lifestyle diet.Recent findings suggest that p53 involved development non-alcoholic disease; however, association between expression remains unclear.Doxorubicin, an anticancer agent, increases p53.Therefore, this study aimed investigate role doxorubicin-induced upregulation free acid (FFA)-induced intracellular lipid accumulation.HepG2 cells were pretreated with 0.5 μg/mL doxorubicin for 12 h, followed treatment FFA (0.5 mM) 24 h induce steatosis.Doxorubicin pretreatment upregulated downregulated endoplasmic reticulum stress-and synthesis-associated genes -treated HepG2 cells.Additionally, AMP-activated protein kinase, key modulator metabolism.Notably, siRNA-targeted knockdown reversed effects cells.Moreover, suppressed -induced spheroids.Conclusively, these results possesses potential application regulation metabolism enhance vitro NAFLD model.

Language: Английский

Isoquercitrin Inhibits Lung Cancer Cell Growth Through Triggering Pyroptosis and Ferroptosis DOI
Haiyin Fan, Pengfei Xu,

Bin Zou

et al.

Nutrition and Cancer, Journal Year: 2024, Volume and Issue: 77(2), P. 299 - 310

Published: Oct. 20, 2024

Isoquercitrin possesses anti-tumor activity in several types of cancers, however, its effects and underlying mechanisms on lung cancer have not been reported. Human cell lines as well normal epithelial BEAS-2B cells were treated with isoquercitrin. The influences isoquercitrin

Language: Английский

Citations

0
Unsaponifiable Matter from Wheat Bran Cultivated in Korea Inhibits Hepatic Lipogenesis by Activating AMPK Pathway DOI Creative Commons
Minju An, Huijin Heo, Jinhee Park

et al.

Foods, Journal Year: 2023, Volume and Issue: 12(21), P. 4016 - 4016

Published: Nov. 3, 2023

Unsaponifiable matter (USM) from wheat bran, a by-product obtained milling, is abundant in health-promoting compounds such as phytosterols, tocopherols, policosanols, and alkylresorcinols. This study aimed to examine the effects of USM bran normal waxy type wheat, Saekeumkang (SKK) Shinmichal (SMC), on hepatic lipid accumulation free fatty acid (FFA)-induced hepatocytes investigate cellular mechanism. The total phytochemical contents were 46.562 g/100 g 38.130 SKK SMC, respectively. FFA treatment increased intracellular by approximately 260% compared control group; however, with SMC significantly attenuated dose-dependent manner. Moreover, downregulated expression lipogenic factors synthase sterol regulatory-element-binding protein 1c 40% group. Treatment promoted lipolysis positively regulated proteins involved β-oxidation, including peroxisome proliferator-activated receptor α its downstream protein, carnitine palmitoyltransferase 1A. blockade AMPK activation abolished inhibitory accumulation. These results indicated that both can alleviate an AMPK-dependent Therefore, may be useful therapeutic intervention for treating metabolic-dysfunction-associated liver disease.

Language: Английский

Citations

1
ß- Caryophyllene Ameliorates Insulin Resistance in Liver of High Fat Diet and Fructose-Induced Type-2 Diabetic Rats DOI Creative Commons

Vadivel Mani,

Sangeeta Chandrashekar,

Jenny Jayapal

et al.

Journal Of Advanced Zoology, Journal Year: 2023, Volume and Issue: 44(4), P. 157 - 165

Published: Nov. 8, 2023

Insulin resistance may impact organs that transmit insulin signals, like skeletal muscle, adipose tissue, and the liver. Hyperglycemia hypertriglyceridemia in fasting state of type 2 diabetes mellitus are more significantly impacted by Hypertriglyceridemia is culprit behind beta-cell failure diabetes. Treating liver as a primary mechanism traditional antidiabetic drugs, but unfortunately drugs has unalterable advisory effect. In present research, addressed with versatile natural phytonutrient beta-caryophyllene to demonstrate effectiveness managing An effective dose -Caryophyllene taken non-parental route (200 mg/kg b.wt) was given for 30 days experimentally induced (high-fat diet fructose-fed) type-2 diabetic rats find out whether β-Caryophyllene regulates IRS-2/akt pathway signaling. rats, data demonstrates ß-caryophyllene treatment increased mRNA protein expression receptor (IR) GLUT-2. However, there significant variation akt receptor-substrate-2 (IRS-2) between groups. Taking caryophyllene supplements can be beneficial control Studies conducted this sesquiterpene could have tremendous potential benefit

Language: Английский

Citations

0
Doxorubicin Attenuates Free Fatty Acid-Induced Lipid Accumulation via Stimulation of p53 in HepG2 Cells DOI Creative Commons
Chawon Yun, Sou Hyun Kim, Doyoung Kwon

et al.

Biomolecules & Therapeutics, Journal Year: 2023, Volume and Issue: 32(1), P. 94 - 103

Published: Dec. 27, 2023

Non-alcoholic fatty liver disease (NAFLD) is characterized by excessive accumulation of fat in the liver, and there a global increase its incidence owing to changes lifestyle diet.Recent findings suggest that p53 involved development non-alcoholic disease; however, association between expression remains unclear.Doxorubicin, an anticancer agent, increases p53.Therefore, this study aimed investigate role doxorubicin-induced upregulation free acid (FFA)-induced intracellular lipid accumulation.HepG2 cells were pretreated with 0.5 μg/mL doxorubicin for 12 h, followed treatment FFA (0.5 mM) 24 h induce steatosis.Doxorubicin pretreatment upregulated downregulated endoplasmic reticulum stress-and synthesis-associated genes -treated HepG2 cells.Additionally, AMP-activated protein kinase, key modulator metabolism.Notably, siRNA-targeted knockdown reversed effects cells.Moreover, suppressed -induced spheroids.Conclusively, these results possesses potential application regulation metabolism enhance vitro NAFLD model.

Language: Английский

Citations

0