Heavy Metals in the Follicular Fluid Affect the Ultrastructure of the Human Mature Cumulus-Oocyte Complex DOI Open Access
Selenia Miglietta, Loredana Cristiano,

Ezio Battaglione

et al.

Published: Oct. 13, 2023

It is known that exposure to heavy metals such as lead (Pb) and cadmium (Cd) has several adverse effects, particularly on the human reproductive system. Pb Cd have been associated with infertility in both men women. In pregnant women, they spontaneous abortion, preterm birth, impairment of development fetus. Since these come from natural anthropogenic activities their harmful effects observed even at levels exposure, remains a public health issue, especially for system. Given this, present study aimed investigate potential follicular fluid (FF) infertile women no-smokers exposed professional reasons or living rural areas near landfills waste disposal areas, correlate intrafollicular presence possible alterations ultrastructure cumulus-oocyte complexes (COCs), probably responsible infertility. Blood FF were measured using atomic absorption spectrometry. COCs corresponding each analyzed subjected ultrastructural analyses by transmission electron microscopy. We demonstrated first time (0.66 µg/dL 0.85 µg/dL) (0.26 µg/L - 0.41 µg/L) could be morphological oocyte CCs ultrastructure. blood (0.54 1.87 above current reference values established guidelines Agency Toxic Substances Disease Registry (ATSDR) Environmental Protection (EPA), whereas (1.28 3.98 below ATSDR we believe due Cd, if cannot exclude additional effect Pb. Our results highlighted oocytes affected maturation quality, showed scarcely active steroidogenic elements. Regressing CCs, cytoplasmic alterations, also numerous. According Cd's endocrine-disrupting activity, poor activity might delayed maturation. So, conclude negatively affect fertilization, embryo development, pregnancy, compromising competence fertilization directly indirectly, impairing CC inducing apoptosis.

Language: Английский

Drug induced liver injury – a 2023 update DOI Creative Commons

Rebecca Allison,

Asha Guraka, Isaac Thom Shawa

et al.

Journal of Toxicology and Environmental Health Part B, Journal Year: 2023, Volume and Issue: 26(8), P. 442 - 467

Published: Oct. 2, 2023

Drug-Induced Liver Injury (DILI) constitutes hepatic damage attributed to drug exposure. DILI may be categorized as hepatocellular, cholestatic or mixed and might also involve immune responses. When occurs in dose-dependent manner, it is referred intrinsic, while if the injury spontaneously, termed idiosyncratic. This review predominately focused on idiosyncratic liver injury. The established molecular mechanisms for include (1) mitochondria dysfunction, (2) increased reactive oxygen species levels, (3) presence of elevated apoptosis necrosis, (4) bile duct injuries associated with mediated pathways. However, should emphasized that underlying responsible are still unknown. Prevention strategies critical incidences occur frequently, treatment options limited once has developed. aim this was utilize retrospective cohort studies from across globe gain insight into epidemiological patterns. considers what currently known regarding DILI, discusses potential risk factors implications coronavirus pandemic presentation research. Future perspectives considered discussed new biomarkers, causality assessment reporting methods.

Language: Английский

Citations

49
Sagittaria sagittifolia polysaccharide extract regulates Nrf2 to improve endoplasmic reticulum stress-mediated apoptosis in rat cataracts and HLEB3 cells DOI

Man-Yu Zhou,

Bingqing Liu,

Xin Gao

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140270 - 140270

Published: Jan. 1, 2025

Language: Английский

Citations

2
Endoplasmic reticulum stress and unfolded protein response: Roles in skeletal muscle atrophy DOI
Yanan Ji, Quan Jiang,

Bingqian Chen

et al.

Biochemical Pharmacology, Journal Year: 2025, Volume and Issue: 234, P. 116799 - 116799

Published: Feb. 12, 2025

Language: Английский

Citations

2
Paeoniflorin protects hepatocytes from APAP-induced damage through launching autophagy via the MAPK/mTOR signaling pathway DOI Creative Commons
Xinyu Deng, Yubing Li, Yuan Chen

et al.

Cellular & Molecular Biology Letters, Journal Year: 2024, Volume and Issue: 29(1)

Published: Sept. 7, 2024

Language: Английский

Citations

12
Exploring drug-induced liver injury: comprehensive insights into mechanisms and management of hepatotoxic agents DOI Creative Commons
Asmaa Saleh,

Thanaa A. El‐Masry,

Aya H. El-Kadem

et al.

Future Journal of Pharmaceutical Sciences, Journal Year: 2025, Volume and Issue: 11(1)

Published: March 28, 2025

Abstract Background Drug-induced liver injury (DILI) is a significant adverse drug reaction, manifesting through range of clinical presentations from mild enzyme to acute failure. Main text This review provides comprehensive overview DILI, emphasizing the differences between intrinsic and idiosyncratic DILI. The underlying molecular mechanisms, like mitochondrial dysfunction, oxidative stress, immune-mediated responses, are discussed in detail. epidemiology DILI explored various retrospective prospective studies, highlighting role specific medications individual susceptibility factors. also addresses challenges diagnosing impact on development practice. Conclusion poses threat due its potential for causing failure associated mortality. To improve patient outcomes, further research crucial identify effective therapeutic interventions.

Language: Английский

Citations

1
Rational construction of a novel fluorescent probe for imaging peroxynitrite in the endoplasmic reticulum during drug-induced liver injury DOI
Caiyun Liu,

Xiaodi Rong,

Yao Wang

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 417, P. 136114 - 136114

Published: June 12, 2024

Language: Английский

Citations

6
Ursodeoxycholic Acid for the Management of Drug-induced Liver Injury: Role of Hepatoprotective and Anti-cholestatic Mechanisms DOI Open Access
Fernando Bessone,

Geraldine L. Hillotte,

Norberto Tamagnone

et al.

Journal of Clinical and Translational Hepatology, Journal Year: 2025, Volume and Issue: 000(000), P. 000 - 000

Published: Jan. 21, 2025

Drug-induced liver injury (DILI) is a harmful reaction to medications, herbs, and dietary supplements that results in dysfunction. Based on the distinct clinical patterns of damage, DILI can be categorized into hepatocellular, cholestatic, mixed types. Hepatocellular linked inflammation, apoptosis, necrosis, while cholestatic commonly associated with bile plugs and, rare cases, ductopenia. Ursodeoxycholic acid (UDCA) therapeutic agent most widely used for treatment hepatopathies diverse etiologies has been mainly as supportive DILI. In this review, we presented more structured systematic framework potential application hepatoprotective across broader range scenarios. A MEDLINE search literature from 1995 present retrieved 41 preliminary studies suggesting UDCA may offer curative preventive benefits hepatocellular well. This aligns preclinical rodents, showing beneficial effects experimental irrespective involved. could due broad potentially UDCA, which address various types damage different causes mechanisms seen all forms UDCA's properties include anticholestatic, antioxidant, anti-inflammatory, anti-apoptotic, anti-necrotic, mitochondrial protective, endoplasmic reticulum stress-relieving, immunomodulatory effects. Controlled use standardized causality assessments are eagerly awaited properly validate Meanwhile, hope article helps clarify systematize versatile safe medication toxicity.

Language: Английский

Citations

0
Development of quercetin-loaded liposomal nanocarriers for alleviation of gemcitabine-induced hepatotoxicity: Optimization, in-vitro, and in-vivo evaluation DOI
Mahran Mohamed Abd El-Emam, Ahmed Alobaida, Marwa Mohamed El Sayed

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: 105, P. 106659 - 106659

Published: Feb. 1, 2025

Language: Английский

Citations

0
CHOP aggravates hepatocyte apoptosis upon endoplasmic reticulum stress by down-regulating autophagy DOI Creative Commons

Jia-Yu Wu,

Bing Han, Ting Yang

et al.

Cell Stress and Chaperones, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Endoplasmic reticulum (ER) stress-associated apoptosis is involved in various liver diseases, including fibrosis, nonalcoholic fatty disease, and cirrhosis. Hepatocytes respond to ER stress by eliciting unfolded protein response (UPR) enhancing autophagy. Autophagy a pivotal mechanism for sustaining normal function, through degradation of damaged fragments removal abnormal aggregates the lumen. Failure restore homeostasis via autophagy harmful hepatocytes contributes apoptosis. Recent findings indicated that C/EBP homologous (CHOP) could exacerbate stress-related down-regulating autophagy, but underlying remains elusive. To investigate impact CHOP on stress-induced rat hepatocytes, potential molecular mechanisms. BRL-3A cells were pre-treated with rapamycin (RAP) 3-methyladenine, then treated dithiothreitol (DTT). Growth apoptotic rates detected using real-time cellular analysis (RTCA) flow cytometry, respectively. molecule levels determined western blotting. CHOP, small interfering RNA, lentivirus vector system used transfect observe gene silencing or overexpression Chromatin immunoprecipitation (ChIP) was confirm whether binds directly ATG12, ATG5, LC3 promotor regions undergoing stress. molecules dramatically upregulated hepatocyte augmented. RAP pre-treatment significantly reduced DTT-induced expression molecules; conversely, 3-MA promoted molecules. Following decreased level autophagy-associated increased, decreased. However, opposite trends observed cells. A negative regulation upon DTT treatment ChIP. enhancement during inhibits promotes apoptosis; however, attenuate Overexpression aggravate

Language: Английский

Citations

0
Precise subcellular organelle-targeted analyses of hepatotoxicity of Polygonum multiflorum DOI Open Access
Yijie Li,

Shuni Duan,

Yinhao Zhang

et al.

Chinese Herbal Medicines, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0