Transcriptomic of mesenchymal stem cells growing on RGD conjugated cotton scaffold confers anti-aging, anti-inflammatory and osteogenesis potentials DOI Creative Commons
Sihem Aouabdi, Taoufik Nedjadi,

Rawiah Alsiary

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 10, 2023

Abstract Background Stem cell therapy represents a good alternative to the degenerated or damaged tissue. cells have been used solely in combination with scaffold. The latter offers structural advantage as it allows grow 3 Dimensional (3-D) conditions mimicking thein vivoenvironments. We previously reported development of 3-D Arginyl-glycyl-aspartic acid (RGD)-conjugated cotton-based scaffold for growth and proliferation mesenchymal stem (MSCs). allowed adhesion MSCs their without compromising viability markers. In depth analysis molecular changes adhering cotton fibers will result better therapy. Our work aims at identifying events happening growing on cotton-RGD conjugated-based through gene expression profiles. this study we seeded 48 hours. Celle were collected RNA, cDNA synthesis whole transcriptomic run both populations. Results revealed several up-regulated down-regulated differently expressed genes compared control cells. Gene ontology identified enriched biological processes, functions, pathway protein-protein interaction genes. Conclusions data suggest that might enhanced potential induce osteogenesis MSCs. results also does not oxidative stress, inflammation aging findings provide useful applications tissue engineering regenerative medicine.

Language: Английский

Polyethersulfone Polymer for Biomedical Applications and Biotechnology DOI Open Access
Monika Wasyłeczko,

Cezary Wojciechowski,

Andrzej Chwojnowski

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(8), P. 4233 - 4233

Published: April 11, 2024

Polymers stand out as promising materials extensively employed in biomedicine and biotechnology. Their versatile applications owe much to the field of tissue engineering, which seamlessly integrates engineering with medical science. In medicine, biomaterials serve prototypes for organ development implants or scaffolds facilitate body regeneration. With growing demand innovative solutions, synthetic hybrid polymer materials, such polyethersulfone, are gaining traction. This article offers a concise characterization polyethersulfone followed by an exploration its diverse biotechnological realms. It concludes summarizing significant roles advancing both medicine biotechnology, outlined accompanying table.

Language: Английский

Citations

14
Simultaneous usage of sulforaphane nanoemulsion and tannic acid in ternary chitosan/gelatin/PEG hydrogel for knee cartilage tissue engineering: In vitro and in vivo study DOI
Mehdi Salehiamin,

Saman Ghoraishizadeh,

Ava Habibpour

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 271, P. 132692 - 132692

Published: May 26, 2024

Language: Английский

Citations

6
Synthesis and biophysical characterization of porous bi-phase calcium phosphate/gelatin-PVA scaffold: Enhanced antibacterial, osteoconductivity and anticancer activity via silver nanoparticles and methotrexate incorporation DOI
Hani Elgharbawy,

A. Hassona,

Mohammed Eid

et al.

Journal of Molecular Structure, Journal Year: 2024, Volume and Issue: 1311, P. 138329 - 138329

Published: April 21, 2024

Language: Английский

Citations

5
Advances in Scaffolds and Additives for Infection Control in Autologous Chondrocyte Transplantation DOI

Nazmia Nassereddine,

Rena Roda,

Rami Mhanna

et al.

Tissue Engineering Part B Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Cartilage tissue engineering (CTE) has revolutionized the field of regenerative medicine, offering significant advancements in surgeries such as autologous chondrocyte transplantation. However, despite these advancements, infections associated with cartilage implants remain a persistent challenge, compromising success and patient recovery. To address challenges, this review provides comprehensive foundation for researchers interested addressing CTE. It begins by briefly outlining major scaffolds currently used CTE distinguishing those antimicrobial properties. Among identified, chitosan chondroitin sulfate stand out their promising compatibility antibacterial The then explores additives that meet three essential criteria: chondrocytes, suitability use scaffolds, efficacy. Chitosan, zinc oxide, silver, copper emerge leading candidates due to chondrocytes proven capabilities. Importantly, criteria were chosen provide practical reliable starting point immediate application. it is acknowledged other modifications fabrication processes bioactive glass graphene which may not fit criteria, also hold potential future research innovation. This underscores need further development enhance infection control measures improve outcomes.

Language: Английский

Citations

0
Advancements in Biomaterials for Cartilage Tissue Engineering: Challenges and Future Directions DOI
Madhan Jeyaraman, Naveen Jeyaraman, Swaminathan Ramasubramanian

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Enhanced biological properties of polyvinyl alcohol-polycaprolactone/hyaluronic acid-coated electrospun scaffolds for articular cartilage regeneration DOI
Omid Fakhraei, Hosein Rostamani, Alberto Abad

et al.

Journal of Biomaterials Science Polymer Edition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 29

Published: April 23, 2025

This study provides a cohesive framework to putting forth PVA-PCL scaffolds coated with hyaluronic acid (HA) hydrogel mimic the characteristics of articular cartilage, as cost-effective tissue engineering alternative. PVA and PCL solutions were prepared electrospun under measured conditions, parameters adjusted fabricate aligned random fiber orientations. Afterward, scaffold was integrated optimal hydrogel, selected for its superior water absorption hydrophilicity. The thickness layer satisfied criteria supporting chondrocyte function, assesses effect on cell viability. Scaffolds characterized using field emission scanning electron microscopy (FE-SEM) morphology, energy-dispersive X-ray spectroscopy (EDX) elemental analysis, Fourier transform infrared (FTIR) chemical composition, tensile tests mechanical behavior. surface wettability determined by contact angle measurements. Biological properties assessed through cytotoxicity, protein assays adhesion visualization distribution DAPI staining, fluorescence microscopy, FE-SEM. Using hydrolytic mechanism, biodegradation pH variations weight loss Accordingly, randomly oriented hydrogel-coated yielded most favorable biological outcomes produce tissue-friendly, biologically robust graft that closely mimics natural cartilage extracellular matrix. pore size these more uniform than those structures. findings suggest possibilities customizing orientation, polymer blending, coating optimize response formation. Combining chondrocytes-seeded hydrogels offers way improve regeneration.

Language: Английский

Citations

0
Fabricated soft materials for cell biology and tissue engineering applications: A review DOI
Xiaoyu Yang, Huimin Cao,

Xian Li

et al.

Materials Today Communications, Journal Year: 2024, Volume and Issue: 38, P. 108563 - 108563

Published: March 1, 2024

Language: Английский

Citations

3
Comparative Study of Autogenic and Allogenic Chondrocyte Transplants on Polyethersulfone Scaffolds for Cartilage Regeneration DOI Open Access

Tomasz Jakutowicz,

Monika Wasyłeczko, Maciej Płończak

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 9075 - 9075

Published: Aug. 21, 2024

The aim of this study was to evaluate the chondrogenic potential chondrocyte transplants cultured in vitro on polyethersulfone (PES) membranes. Forty-eight rabbits (96 knee joints) were used project. synthetic, macro-porous PES membranes as scaffolds. Fragments articular cartilage harvested from non-weight-bearing areas joints animals. Chondrocytes isolated and then cultivated scaffolds for 3 weeks. animals divided into four groups. All lesions full thickness defects. In Group I, autogenic chondrocytes transplanted defect area; II, allogenic III, pure IV, left untreated. Half each group terminated after 8 weeks, remaining half 12 weeks postoperatively. samples underwent macroscopic evaluation using Brittberg scale microscopic O’Driscoll scale. best regeneration observed Groups II I. results achieved with two surgeries, while only one operation needed. This indicates that do not require highlighting importance further vivo studies better understand advantage. success desired properties are attributed mainly presence sulfonic groups structure material. These groups, similar chondroitin sulfate, which naturally occurs hyaline cartilage, likely enable mutual affinity between scaffold cells promote colonization by cells.

Language: Английский

Citations

1
Transcriptomics Demonstrates Significant Biological Effect of Growing Stem Cells on RGD-Cotton Scaffold DOI
Sihem Aouabdi, Taoufik Nedjadi,

Rawiah Alsiary

et al.

Tissue Engineering Part A, Journal Year: 2024, Volume and Issue: 30(15-16), P. 485 - 498

Published: April 26, 2024

Stem cell therapy provides a viable alternative treatment for degenerated or damaged tissue. cells have been used either alone in conjunction with an artificial scaffold. The latter structural advantage by enabling the to thrive three-dimensional (3D) settings, closely resembling natural

Language: Английский

Citations

0
Tissue engineering and future directions in regenerative medicine for knee cartilage repair: a comprehensive review DOI Creative Commons
Dragan Primorac, Vilim Molnar, Dimitrios Tsoukas

et al.

Croatian Medical Journal, Journal Year: 2024, Volume and Issue: 65(3), P. 268 - 288

Published: June 1, 2024

This review evaluates the current landscape and future directions of regenerative medicine for knee cartilage repair, with a particular focus on tissue engineering strategies. In this context, scaffold-based approaches have emerged as promising solutions regeneration. Synthetic scaffolds, while offering superior mechanical properties, often lack biological cues necessary effective integration. Natural though biocompatible biodegradable, frequently suffer from inadequate strength. Hybrid combining elements both synthetic natural materials, present balanced approach, enhancing support functionality. Advances in decellularized extracellular matrix scaffolds shown potential promoting cell infiltration integration native tissues. Additionally, bioprinting technologies enabled creation complex, bioactive that closely mimic zonal organization cartilage, providing an optimal environment growth differentiation. The also explores gene therapy editing techniques, including CRISPR-Cas9, to enhance repair by targeting specific genetic pathways involved these advanced therapies holds promise developing personalized durable treatments injuries osteoarthritis. conclusion, underscores importance continued multidisciplinary collaboration advance innovative bench bedside improve outcomes patients damage.

Language: Английский

Citations

0