Transcriptomic of mesenchymal stem cells growing on RGD conjugated cotton scaffold confers anti-aging, anti-inflammatory and osteogenesis potentials DOI Creative Commons
Sihem Aouabdi, Taoufik Nedjadi,

Rawiah Alsiary

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 10, 2023

Abstract Background Stem cell therapy represents a good alternative to the degenerated or damaged tissue. cells have been used solely in combination with scaffold. The latter offers structural advantage as it allows grow 3 Dimensional (3-D) conditions mimicking thein vivoenvironments. We previously reported development of 3-D Arginyl-glycyl-aspartic acid (RGD)-conjugated cotton-based scaffold for growth and proliferation mesenchymal stem (MSCs). allowed adhesion MSCs their without compromising viability markers. In depth analysis molecular changes adhering cotton fibers will result better therapy. Our work aims at identifying events happening growing on cotton-RGD conjugated-based through gene expression profiles. this study we seeded 48 hours. Celle were collected RNA, cDNA synthesis whole transcriptomic run both populations. Results revealed several up-regulated down-regulated differently expressed genes compared control cells. Gene ontology identified enriched biological processes, functions, pathway protein-protein interaction genes. Conclusions data suggest that might enhanced potential induce osteogenesis MSCs. results also does not oxidative stress, inflammation aging findings provide useful applications tissue engineering regenerative medicine.

Language: Английский

Transcriptomics Demonstrates Significant Biological Effect of Growing Stem Cells on RGD-Cotton Scaffold DOI
Sihem Aouabdi, Taoufik Nedjadi,

Rawiah Alsiary

et al.

Tissue Engineering Part A, Journal Year: 2024, Volume and Issue: 30(15-16), P. 485 - 498

Published: April 26, 2024

Stem cell therapy provides a viable alternative treatment for degenerated or damaged tissue. cells have been used either alone in conjunction with an artificial scaffold. The latter structural advantage by enabling the to thrive three-dimensional (3D) settings, closely resembling natural

Language: Английский

Citations

0
Transcriptomic of mesenchymal stem cells growing on RGD conjugated cotton scaffold confers anti-aging, anti-inflammatory and osteogenesis potentials DOI Creative Commons
Sihem Aouabdi, Taoufik Nedjadi,

Rawiah Alsiary

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 10, 2023

Abstract Background Stem cell therapy represents a good alternative to the degenerated or damaged tissue. cells have been used solely in combination with scaffold. The latter offers structural advantage as it allows grow 3 Dimensional (3-D) conditions mimicking thein vivoenvironments. We previously reported development of 3-D Arginyl-glycyl-aspartic acid (RGD)-conjugated cotton-based scaffold for growth and proliferation mesenchymal stem (MSCs). allowed adhesion MSCs their without compromising viability markers. In depth analysis molecular changes adhering cotton fibers will result better therapy. Our work aims at identifying events happening growing on cotton-RGD conjugated-based through gene expression profiles. this study we seeded 48 hours. Celle were collected RNA, cDNA synthesis whole transcriptomic run both populations. Results revealed several up-regulated down-regulated differently expressed genes compared control cells. Gene ontology identified enriched biological processes, functions, pathway protein-protein interaction genes. Conclusions data suggest that might enhanced potential induce osteogenesis MSCs. results also does not oxidative stress, inflammation aging findings provide useful applications tissue engineering regenerative medicine.

Language: Английский

Citations

0