Micropropagation and genetic transformation of Byblis liniflora DOI Creative Commons

Alberto Coronado-Martín,

Constanza Martin-Vásquez,

Marybel Jáquez

et al.

In Vitro Cellular & Developmental Biology - Plant, Journal Year: 2024, Volume and Issue: 60(5), P. 634 - 645

Published: Aug. 6, 2024

Abstract Byblis , a small genus of carnivorous plants predominantly found in Australia, is characterized by its passive trapping mechanism and unique floral features. The chemical composition including identified phenylethanoid glycosides, particularly acteoside, highlights pharmacological potential with various biological activities. In vitro culture techniques have been established for propagation, micropropagation protocols developed different species. However, information on genetic transformation, vital trait modification enhanced interest, remains limited. This study focuses optimizing micropropagation, adventitious regeneration, transformation methods liniflora . Adventitious regeneration rates were highest medium reduced Murashige Skoog salts (MS/10) sucrose (3 gL −1 ) concentrations. Zeatin supplementation (1 mgL further improved bud development 100% regenerated root explants 8.8 shoots per explant. Liquid MB3 supplemented indole-3-acetic acid (IAA) 5 facilitated efficient rooting acclimatization. establishment an Rhizobium -mediated method yielded transgenic expressing green fluorescent protein (GFP). Molecular analysis confirmed transgene integration, marking the first successful genus. These advancements pave way exploring gene function enhancing properties, thereby broadening our understanding utilization like

Language: Английский

Adapting to a Shifting Planet: The future of Drosera Species amidst global challenges and conservation imperatives. DOI
Ulises Olivares‐Pinto, João Pedro Lopes, C. Ruiz-Aguilar

et al.

Anthropocene, Journal Year: 2025, Volume and Issue: unknown, P. 100466 - 100466

Published: Feb. 1, 2025

Language: Английский

Citations

0
The Occurrence, Uses, Biosynthetic Pathway, and Biotechnological Production of Plumbagin, a Potent Antitumor Naphthoquinone DOI Creative Commons
P. B. Kavi Kishor, Bangaru Naidu Thaddi, G. Rajasheker

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(7), P. 1618 - 1618

Published: April 4, 2025

Plumbagin is an important naphthoquinone with potent anticancer properties besides multitudinous uses in healthcare. It produced a limited number of species and families but mostly the roots Plumbaginaceae family members. The biosynthetic pathway genes that regulate plumbagin synthesis are not completely known, details these being revealed. Several species, including Plumbago, Drosera, others, uprooted for extraction by pharmaceutical industries, leading to destruction natural habitats. industry therefore facing acute shortage plant material. This necessitates enhancing accumulation using suspensions hairy meet market demands. Many factors, such as aggregate size inoculum, stability culture, sequential effects elicitors, immobilization, permeabilization, have been demonstrated act synergistically markedly augment accumulation. Hairy root cultures can be used large-scale production, growth, accumulation, exploration their efficacy now imperative. secretion compounds into spent medium situ adsorption via resin has remarkable potential, this thoroughly exploited. Improvements quality biomass, selection cell lines, production bioreactors thus far sporadic, parameters need further In review, we report advances made relating importance stable line long-term cultures, plumbagin, its semicontinuous total recycling different types bioreactors. Such might pave way industrial exploitation. steps currently understood also aid us isolating relevant order examine overexpression or heterologous downregulation edit genome CRISPR-Cas9 technology enhance plumbagin. Its potential molecule mode action amply demonstrated, exploited clinics due insolubility water highly lipophilic nature. Plumbagin-loaded nanoemulsions, plumbagin–silver, albumin nanoparticle formulations overcome problems solubility tried improve bioavailability antiproliferative activities, discussed current paper.

Language: Английский

Citations

0
Inhibition of RPA32 and Cytotoxic Effects of the Carnivorous Plant Sarracenia purpurea Root Extract in Non-Small-Cell Lung Cancer Cells DOI Creative Commons

Kuo-Ting Chang,

Yu‐Cheng Chen, Yi Lien

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(10), P. 1426 - 1426

Published: May 9, 2025

The carnivorous plant Sarracenia purpurea has been traditionally used in various ethnobotanical applications, including treatments for type 2 diabetes and tuberculosis-like symptoms. This study investigates the cytotoxic effects of S. root extract (Sp-R) on human non-small-cell lung cancer (NSCLC) cell lines, H1975, H838, A549, focusing its impact survival, apoptosis, proliferation, migration. Additionally, ability to inhibit single-stranded DNA-binding activity RPA32 (huRPA32), a key protein DNA replication, was evaluated. Extracts from different parts (leaf, stem, root) were prepared using solvents (water, methanol, ethanol, acetone) screened apoptosis-inducing potential chromatin condensation assay. Among these, acetone-extracted fraction (Sp-R-A) exhibited most potent pro-apoptotic effects. MTT assay demonstrated dose-dependent effect NSCLC cells, with IC50 values 33.74 μg/mL 60.79 66.52 A549. Migration clonogenic assays further revealed that Sp-R-A significantly inhibited migration colony formation manner. Moreover, enhanced apoptosis when combined EGFR inhibitor afatinib, suggesting synergistic effect. electrophoretic mobility shift confirmed huRPA32, an 13.6 μg/mL. AlphaFold structural prediction molecular docking studies indicated major bioactive compounds purpurea, α-amyrin, ursolic acid, betulinaldehyde, strongly interact domain potentially contributing inhibitory Overall, these findings suggest huRPA32 is target anticancer against highlighted, supporting investigation into therapeutic applications.

Language: Английский

Citations

0
Shoots and Turions of Aquatic Plants as a Source of Fatty Acids DOI Creative Commons
Maciej Strzemski, Lubomı́r Adamec, Sławomir Dresler

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(9), P. 2062 - 2062

Published: April 29, 2024

Background: Fatty acids are essential for human health. Currently, there is a search alternative sources of fatty that could supplement such as staple crops or fishes. Turions aquatic plants accumulate variety substances starch, free sugars, amino acids, reserve proteins and lipids. Our aim to see if turions can be valuable source acids. Methods: Overwintering shoots carnivorous were collected. The plant material was extracted with hexane. oils analyzed using gas chromatograph mass spectrometer. Results: dominant compound in all samples linolenic acid. oil content different shoots. the higher than turions, but proportion from low contrast turions. Utricularia species shown composed about 50% Conclusions: used obtain unsaturated In addition, high acid may explain their ability survive at temperatures.

Language: Английский

Citations

2
Plumbagin's Antiproliferative Mechanism in Human Cancer Cells: A Copper‐Dependent Cytotoxic Approach DOI Creative Commons
Mohamed El Oirdi

Chemical Biology & Drug Design, Journal Year: 2024, Volume and Issue: 104(2)

Published: Aug. 1, 2024

ABSTRACT Cancer is a serious global health problem, causing the loss of millions lives each year. Plumbagin, compound derived from medicinal plant Plumbago zeylanica , has shown promise in stopping growth tumor cells both laboratory settings and living organisms. Many plant‐based compounds exert their effects through copper's ability to produce reactive oxygen species (ROS). This study aimed understand how plumbagin, dependent on copper, induces cell death (apoptosis) human cancer various experiments. The results demonstrate that plumbagin hinders pancreatic PNAC‐1 MIA PaCa‐2 by utilizing copper naturally present cells. Unlike metal chelators remove iron zinc (desferrioxamine mesylate histidine), specific chelator called neocuproine lessens caused plumbagin. When ROS scavengers are used, plumbagin‐induced apoptosis inhibited, indicating plays role initiating death. also proves prevents leaving suppressing expression genes ( CTR1 ATP7A ). It confirmed targets nuclear leading signals promote oxidative stress and, ultimately, These findings provide valuable insights into potential as substance combat cancer, highlighting importance understanding behaves within

Language: Английский

Citations

1
Nitric oxide action in the digestive fluid of Nepenthes × ventrata is linked to the modulation of ROS level DOI Creative Commons
Agnieszka Wal, Maciej Piekarniak, Paweł Staszek

et al.

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 216, P. 109088 - 109088

Published: Sept. 3, 2024

Nepenthes are carnivorous plants with photoactive leaves converted into jug-shaped containers filled the digestive fluid. The digestion requires various enzymes and reactive oxygen species (ROS) that facilitate proteolysis. Reactive nitrogen present in fluid of × ventrata, increased nitric oxide (NO) formation is associated protein degradation. aim work was to verify beneficial effect NO application trap on dynamics ROS homeostasis. Measurements were done using or tissue collected from mature pitcher (fed) grown a greenhouse. Two independent methods confirmed fed non-fed traps. supplementation food accelerated degradation by increasing proteolytic activity. modulated free radical (as result direct impact NADPH oxidase), stimulated scavenging capacity, -SH groups flavonoids content, particularly at beginning digestion. In traps, relatively high level nitration may prevent self-protein Whereas, after initiation decreasing nitrated proteins indicate their Therefore, it can be assumed exhibits protective before digestion, while during an accelerator decomposition balance keeper.

Language: Английский

Citations

1
Micropropagation and genetic transformation of Byblis liniflora DOI Creative Commons

Alberto Coronado-Martín,

Constanza Martin-Vásquez,

Marybel Jáquez

et al.

In Vitro Cellular & Developmental Biology - Plant, Journal Year: 2024, Volume and Issue: 60(5), P. 634 - 645

Published: Aug. 6, 2024

Abstract Byblis , a small genus of carnivorous plants predominantly found in Australia, is characterized by its passive trapping mechanism and unique floral features. The chemical composition including identified phenylethanoid glycosides, particularly acteoside, highlights pharmacological potential with various biological activities. In vitro culture techniques have been established for propagation, micropropagation protocols developed different species. However, information on genetic transformation, vital trait modification enhanced interest, remains limited. This study focuses optimizing micropropagation, adventitious regeneration, transformation methods liniflora . Adventitious regeneration rates were highest medium reduced Murashige Skoog salts (MS/10) sucrose (3 gL −1 ) concentrations. Zeatin supplementation (1 mgL further improved bud development 100% regenerated root explants 8.8 shoots per explant. Liquid MB3 supplemented indole-3-acetic acid (IAA) 5 facilitated efficient rooting acclimatization. establishment an Rhizobium -mediated method yielded transgenic expressing green fluorescent protein (GFP). Molecular analysis confirmed transgene integration, marking the first successful genus. These advancements pave way exploring gene function enhancing properties, thereby broadening our understanding utilization like

Language: Английский

Citations

0