Hydrophilic/hydrophobic modified microchip for detecting multiple gene doping candidates using CRISPR-Cas12a and RPA

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 263, P. 116631 - 116631

Published: Aug. 3, 2024

Language: Английский

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Ethical Aspects of Human Genome Research in Sports—A Narrative Review

Genes, Journal Year: 2024, Volume and Issue: 15(9), P. 1216 - 1216

Published: Sept. 18, 2024

Human genome research in sports raises complex ethical considerations regarding the intersection of genetics and athletic performance. Pursuing genetic enhancements must uphold fairness, equality, respect for human dignity. This narrative review explores dimensions sports, its potential implications on athletes, integrity sports. As a review, this study synthesizes existing literature expert insights to examine aspects extensively examined current genetics, performance, concerns, rights, legal regulations within European context. The was searched using SPORTDiscus, Scopus, Google Scholar, PubMed databases. Exploring reveals significant implications, including discrimination, impacts creating underclass athletes. There are also definite benefits surrounding testing. In conclusion, contends that integrating into developing applying technologies is crucial upholding fundamental principles It stresses importance open inclusive dialogue about consequences advancements future generations,

Language: Английский

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Gene doping detection in the era of genomics

Drug Testing and Analysis, Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 25, 2024

Recent progress in gene editing has enabled development of therapies for many genetic diseases, but also made doping an emerging risk sports and competitions. By delivery exogenous transgenes into human body, not only challenges competition fairness places health on athletes. World Anti-Doping Agency (WADA) clearly inhibited the use cell sports, techniques have been developed detection. In this review, we will summarize main tools detection at present, highlight current tools, elaborate future utilizations high-throughput sequencing unbiased, sensitive, economic large-scale detections. Quantitative real-time PCR assays are widely used methods which useful known targets vulnerable to codon optimization exon-exon junction sites transgenes. High-throughput become a powerful tool various applications life research, era genomics it possible sensitive Non-biased genomic profiling could efficiently detect new targets, low-input amplification long-read third-generation application potentials more efficient straightforward closely monitoring scientific advancements sport genetics, play important role hopefully contribute doping-free future.

Language: Английский

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Bioanalytical methods in doping controls: a review

Bioanalysis, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 12

Published: Feb. 7, 2025

The analytical and technological approaches employed in doping analysis are constantly reviewed updated to allow for keeping pace with progresses pharmaceutical medicinal research the therein inherent options of misuse as performance enhancing drugs or methods. Enormous changes, improvements, developments have been achieved recent years, particularly, but not exclusively, bioanalytical sector. Several these new strategies examined systematically this review using examples from World Anti-Doping Agency (WADA) list banned substances includes, among others, application sophisticated in-vitro models mimicking multi compartment models, investigation into long-term metabolites anabolic agents, impact a distinct gene mutation on erythropoietin, studies development therapeutic protein-based myostatin inhibiting properties, methods applying molecular biological section used uncover doping, finally uncovering prohibited use autologous blood transfusion. All challenges investigations support ongoing progress modern controls future will help fill gap between advance cheating athletes sport drug testing.

Language: Английский

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Recent advances of optical and electrochemical (bio)sensors for doping agents detection in sport

Journal of Industrial and Engineering Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

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Recent advances and future perspectives of biosensing technologies in anti-doping applications

Bioanalysis, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 4

Published: March 24, 2025

Language: Английский

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Pharmacological and toxicological aspects of doping substances and the role of the sports pharmacist

Hospital Pharmacology - International Multidisciplinary Journal, Journal Year: 2025, Volume and Issue: 12(1), P. 1599 - 1619

Published: Jan. 1, 2025

Language: Английский

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Characterizing Lomerizine metabolites in camel urine: High‐resolution mass spectrometry method development and validation for enhanced doping control

Rapid Communications in Mass Spectrometry, Journal Year: 2024, Volume and Issue: 38(17)

Published: June 24, 2024

Rationale Lomerizine (LMZ) is an antimigraine drug that works as a calcium channel blocker and has selective effects on the central nervous system. It metabolized into trimetazidine (TMZ), which prohibited substance owing to its performance‐enhancing in both human animal sports. Effective doping control measures are imperative distinguish source of TMZ samples ensure integrity fairness sport, therefore comprehensive analysis LMZ metabolites essential identify potential biomarkers camel urine for effective control. Methods Camel were collected from four healthy animals following single oral administration at dosage 1 mg/kg body weight. In vitro studies conducted using homogenized liver samples. extracted solid‐phase extraction analyzed with Thermo Fisher Orbitrap Exploris liquid chromatography mass spectrometry The acquired data was processed Compound Discoverer software. Results study camels identified 10 phase I one II metabolites. primary pathway formation de‐alkylation, while metabolite formed through alkylation parent drug. provided valuable insights unique metabolic pathways under specific experimental conditions. Conclusion developed method enables detection characterization camels. act marker distinctive efficient analytical strategies routine applications.

Language: Английский

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Doping Detection Based on the Nanoscale: Biosensing Mechanisms and Applications of Two-Dimensional Materials

Biosensors, Journal Year: 2025, Volume and Issue: 15(4), P. 227 - 227

Published: April 3, 2025

Doping undermines fairness in sports and threatens athlete health, while conventional detection methods like LC-MS GC-MS face challenges such as complex procedures, matrix interferences, lengthy processing times, limiting on-site applications. Two-dimensional (2D) materials, including graphene, MoS2, metal–organic frameworks (MOFs), offer promising solutions due to their large surface areas, tunable electronic structures, special interactions with doping agents, hydrogen bonding, π-π stacking, electrostatic forces. These materials enable signal transduction through changes conductivity or fluorescence quenching. This review highlights the use of 2D detection. For example, reduced graphene oxide–MOF composites show high sensitivity for detecting anabolic steroids testosterone, NiO/NGO nanocomposites exhibit strong selectivity stimulants ephedrine. However, environmental instability production costs hinder widespread application. Future efforts should focus on improving material stability chemical modifications, reducing costs, integrating these into advanced systems machine learning. Such advancements could revolutionize detection, ensuring protecting health.

Language: Английский

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Development and validation of a quantitative UHPLC-HRMS bioanalytical method for equine anti-doping control

Journal of Pharmacological and Toxicological Methods, Journal Year: 2025, Volume and Issue: unknown, P. 107759 - 107759

Published: May 1, 2025

Language: Английский

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