MICRONUCLEI AS GENOTOXICITY ENDPOINT APPLIED IN THE CO-CULTURE OF TWO MAMMALIAN CELL LINES DOI Creative Commons
Naji Said Aboud Hadi, Helga Stopper

Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Journal Year: 2024, Volume and Issue: 901, P. 503839 - 503839

Published: Dec. 12, 2024

There has been a shift from traditional animal models towards alternative methods. While 2D cell culture decade long tradition, more advances methods like 3D cultures, organoids, and co-culture techniques, which better mimic in vivo conditions, are not yet well established every research area. Genotoxicity assessment is an integral part of toxicological testing or regulatory approval pharmaceuticals chemicals. The micronucleus assay now standard method this context. In systematic literature review, we aim to describe the state art application co-cultures two mammalian lines for assessment. We summarized types used, co-culture, disease agents, as additional genotoxicity endpoints viability tests. Airway system cells were most frequent, followed by macrophage-like cells, liver various others. Co-culture techniques involve either direct physical contact separation porous membranes. Within limited number investigations using other assays comet γH2AX parallel, performed well. Overall, test demonstrating its suitability complex substance beyond simple encouraging widespread use systems future.

Language: Английский

Nano/micro-plastic, an invisible threat getting into the brain DOI Creative Commons
Ajeet Kaushik, Avtar Singh, Vijay Kumar Gupta

et al.

Chemosphere, Journal Year: 2024, Volume and Issue: 361, P. 142380 - 142380

Published: May 18, 2024

Due to weather and working/operational conditions, plastic degradation produces toxic non-biodegradable nano microplastics (N/M-Ps, ranging from 10 nm 5 mm), over time these N/M-Ps have integrated with the human cycle through ingestion inhalation. These N/M-Ps, as serious emerging pollutants, are causing considerable adverse health issues due up-taken by cells, tissue, organs, including brain. It has been proven that can cross blood-brain barrier (via olfactory blood vessels) affect secretion of neuroinflammatory (cytokine chemokine), transporters, receptor markers. Neurotoxicity, neuroinflammation, brain injury, which may result in such scenarios a concern cause disorders. However, related pathways pathogenesis not well-explored but focus upcoming research. Therefore, this editorial, well-organized multidisciplinary research is required explore associated pathogenesis, leading mapping nano-enabled therapeutics acute chronic N/M-P exposure.

Language: Английский

Citations

12
Synergistic Enhancement of Carboplatin Efficacy through pH-Sensitive Nanoparticles Formulated Using Naturally Derived Boswellia Extract for Colorectal Cancer Therapy DOI Creative Commons
Sherif Ashraf Fahmy, Nada K. Sedky, Hatem A. F. M. Hassan

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(10), P. 1282 - 1282

Published: Sept. 30, 2024

Carboplatin (Cp) is a potent chemotherapeutic agent, but its effectiveness constrained by associated side effects. Frankincense, an oleo-gum resin from the

Language: Английский

Citations

4
Toxicological mechanisms and molecular impacts of tire particles and antibiotics on zebrafish DOI

Jingya Wen,

Jiaxuan Gao,

Yajing Liu

et al.

Environmental Pollution, Journal Year: 2024, Volume and Issue: 362, P. 124912 - 124912

Published: Sept. 7, 2024

Language: Английский

Citations

1
MICRONUCLEI AS GENOTOXICITY ENDPOINT APPLIED IN THE CO-CULTURE OF TWO MAMMALIAN CELL LINES DOI Creative Commons
Naji Said Aboud Hadi, Helga Stopper

Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Journal Year: 2024, Volume and Issue: 901, P. 503839 - 503839

Published: Dec. 12, 2024

There has been a shift from traditional animal models towards alternative methods. While 2D cell culture decade long tradition, more advances methods like 3D cultures, organoids, and co-culture techniques, which better mimic in vivo conditions, are not yet well established every research area. Genotoxicity assessment is an integral part of toxicological testing or regulatory approval pharmaceuticals chemicals. The micronucleus assay now standard method this context. In systematic literature review, we aim to describe the state art application co-cultures two mammalian lines for assessment. We summarized types used, co-culture, disease agents, as additional genotoxicity endpoints viability tests. Airway system cells were most frequent, followed by macrophage-like cells, liver various others. Co-culture techniques involve either direct physical contact separation porous membranes. Within limited number investigations using other assays comet γH2AX parallel, performed well. Overall, test demonstrating its suitability complex substance beyond simple encouraging widespread use systems future.

Language: Английский

Citations

0