Symmetry,
Journal Year:
2024,
Volume and Issue:
16(12), P. 1676 - 1676
Published: Dec. 18, 2024
Phosphonates
derivatives
are
compounds
of
interests
and
applied
as
drugs
of,
e.g.,
antibacterial
antiviral
activities,
connected
with
their
inhibitory
activity
towards
different
enzymes,
which
is
related
to
the
configuration
particular
compound
isomers.
The
biological
synthesis
such
molecules
method
choice
can
be
carried
out
using
enzymes
or
whole
cells
from
organisms.
Photobiocatalysts
employed
in
bioconversion
epoxymethyl
dimethyl
phosphonate
able
convert
this
substrate
into
a
pure
geometric
isomer
unsaturated
product,
(1E)-3-hydroxyprop-1-enylphosphonate,
rare
expensive
high
added
value.
Six
strains
were
screened
epoxy
case
Synechococcus
bigranulatus,
over
99%
conversion
was
achieved.
product
structure
confirmed
Mass
Spectroscopy
(MS);
1H,
13C,
31P,
2D
Nuclear
Magnetic
Resonance
(NMR);
Infrared
(IR).
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 19, 2024
A
bstract
Phosphonates
(PHTs),
organic
compounds
with
a
stable
C—P
bond,
are
widely
distributed
in
nature.
Glyphosate
(GP),
synthetic
PHT,
is
extensively
used
agriculture
and
has
been
linked
to
various
human
health
issues
environmental
damage.
Given
the
prevalence
of
GP,
developing
cost-effective,
on-site
methods
for
GP
detection
key
assessing
pollution
reducing
exposure
risks.
We
adopted
Agrobacterium
tumefaciens
CHLDO,
natural
degrader,
as
source
genetic
parts
constructing
PHT
biosensors.
In
this
species,
phn
gene
cluster,
encoding
lyase
pathway,
regulated
by
PhnF
transcriptional
repressor
part
Pho
regulon.
selected
phnG
promoter,
which
displays
dose-dependent
response
build
set
whole-cell
Through
stepwise
optimization
cascade,
we
created
biosensor
capable
detecting
0.25-50
μM
range
samples,
including
soil
water.
ACS Synthetic Biology,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 30, 2024
Phosphonates
(PHTs),
organic
compounds
with
a
stable
C-P
bond,
are
widely
distributed
in
nature.
Glyphosate
(GP),
synthetic
PHT,
is
extensively
used
agriculture
and
has
been
linked
to
various
human
health
issues
environmental
damage.
Given
the
prevalence
of
GP,
developing
cost-effective,
on-site
methods
for
GP
detection
key
assessing
pollution
reducing
exposure
risks.
We
adopted
Symmetry,
Journal Year:
2024,
Volume and Issue:
16(12), P. 1676 - 1676
Published: Dec. 18, 2024
Phosphonates
derivatives
are
compounds
of
interests
and
applied
as
drugs
of,
e.g.,
antibacterial
antiviral
activities,
connected
with
their
inhibitory
activity
towards
different
enzymes,
which
is
related
to
the
configuration
particular
compound
isomers.
The
biological
synthesis
such
molecules
method
choice
can
be
carried
out
using
enzymes
or
whole
cells
from
organisms.
Photobiocatalysts
employed
in
bioconversion
epoxymethyl
dimethyl
phosphonate
able
convert
this
substrate
into
a
pure
geometric
isomer
unsaturated
product,
(1E)-3-hydroxyprop-1-enylphosphonate,
rare
expensive
high
added
value.
Six
strains
were
screened
epoxy
case
Synechococcus
bigranulatus,
over
99%
conversion
was
achieved.
product
structure
confirmed
Mass
Spectroscopy
(MS);
1H,
13C,
31P,
2D
Nuclear
Magnetic
Resonance
(NMR);
Infrared
(IR).
