Effects of Drying Methods on Morphological Characteristics, Metabolite Content, and Antioxidant Capacity of Cordyceps sinensis

Foods, Journal Year: 2024, Volume and Issue: 13(11), P. 1639 - 1639

Published: May 24, 2024

Cordyceps sinensis is a rare and endangered medicinal herb in China typical food plant. Most of the research related to focuses on its pharmacological effects, artificial cultivation clinical applications. However, there are few comprehensive evaluations quality under different drying methods. In this study, effects vacuum freeze-drying (DG), oven-drying (HG) air-drying (YG) morphological characteristics, microstructure, antioxidant activity metabolites were investigated using wild as object. The results showed that their appearance morphology, YG- HG-method samples darker color wilted, while DG- method golden yellow had better fullness. terms stomata YG HG relatively small irregularly shaped, whereas those DG larger neat. capacity, lowest, followed by group, group highest total capacity. A correlation analysis revealed significant relationship between capacity lipids, lipid molecules, nucleosides, nucleotides, analogs. metabolomics identified 1937 from 18 superclasses, with lipid-like organic acids derivatives, organoheterocyclic compounds, oxygen compounds being predominant sinensis. Differentially accumulated (DAMs) higher levels lipids compared other methods, suggesting optimal preservation for These findings offer insights selecting appropriate methods maintaining post-drying

Language: Английский

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Comparative Metabolite Profiling Between Cordyceps sinensis and Other Cordyceps by Untargeted UHPLC-MS/MS

Biology, Journal Year: 2025, Volume and Issue: 14(2), P. 118 - 118

Published: Jan. 23, 2025

Cordyceps sinensis is a second-class, nationally protected, medicinal fungi and serves as functional nutrient in China. C. extremely scarce due to its peculiar growing environment the extensive gathering practices carried out by humans. A large number of counterfeit products for this have also emerged market. At present, there lack research on differential metabolites products. The current study used an LC-MS non-targeted metabolomics method compare differences between other Cordyceps. results indicated that were significant others. 18 superclasses found differences, involving lipids, organic acids, nucleosides, carbohydrates, amino vitamins, their derivatives. Compared with four groups Cordyceps, 8 screened. In addition, types abundance different nucleosides superior compared (e.g., 5-Methyldioxycytidine, didanosine, cytidine, etc.). Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analysis showed metabolism arginine proline, glycerophosphate two metabolic pathways provide reference identifying authenticity using methods.

Language: Английский

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Impact of climate change on the distribution of Isaria cicadaeMiquel in China: predictions based on the MaxEnt model

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 7, 2025

Introduction Isaria cicadae , a historically valued edible and medicinal fungus in China, has been experiencing critical decline abundance due to ecological degradation overexploitation. Understanding its potential distribution is essential for promoting sustainable harvesting practices. Methods This study utilizes the MaxEnt model, combined with known records 22 environmental variables, predict of I. under three representative emission scenarios (CMIP6: SSP1-2.6, SSP2-4.5, SSP5-8.5) 2050s 2070s. Results The analysis identifies seven key variables influencing habitat suitability : mean temperature driest quarter (bio09), wettest (bio08), precipitation month (bio16), diurnal range (bio02), isothermality (bio03), elevation, slope. Currently, mainly found provinces Yunnan, Sichuan, Hunan, Hubei, Guizhou, Jiangxi, Guangxi, Fujian, Anhui, Zhejiang, Yunnan Sichuan having largest areas high at 25.79 × 10 4 km 2 21.36 respectively. Discussion Guangxi Zhuang Autonomous Region are identified as primary regions suitability. aims further elucidate impact environment on from geographical perspective provide theoretical insights future cultivation conservation strategies this species.

Language: Английский

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Analysis of Metabolic Profiles and Antioxidant Activity of Chinese Cordyceps, Ophiocordyceps sinensis, and Paecilomyces hepiali Based on Untargeted Metabolomics

Biology, Journal Year: 2024, Volume and Issue: 13(9), P. 683 - 683

Published: Aug. 31, 2024

Chinese cordyceps (GL) is a traditional medicinal fungus, with Ophiocordyceps sinensis (O. sinensis, BL) and Paecilomyces hepiali (P. hepiali, JSB) being fungi isolated from wild cordyceps. These three species share similar chemical composition pharmacological effects. Existing studies have primarily compared the metabolites of O. overlooking assessment antioxidant capacity in cordyceps, P. sinensis. In this study, LC-MS/MS was employed to analyze GL, JSB, BL. Utilizing principal component analysis (PCA), supervised orthogonal partial least squares discriminant (OPLS-DA), hierarchical cluster (HCA), it observed that majority differential (DMs) accumulated organic acids derivatives, lipids lipid-like molecules, organoheterocyclic compounds. Antioxidant activity indicated GL exhibited higher 2,2-diphenyl-1-picrylhydrazyl radical scavenging ability (DPPH•, rate 81.87 ± 0.97%), hydroxyl free (•OH, 98.10 0.60%), superoxide anion (O2•−, 69.74 4.36%), while JSB demonstrated FRAP total 8.26 μmol Trolox/g (p < 0.05). Correlation revealed positive correlation between DMs (fatty acyls amino acids) DPPH•, FRAP, •OH, O2•− Additionally, glycerophospholipid were found be positively correlated Through KEGG pathway analysis, determined accumulation pathways such as cutin, suberine wax biosynthesis has impact on influencing samples. results shed light metabolic characteristics its substitutes offer valuable insights into how different strength activity, aiding advancement application substitutes.

Language: Английский

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Exploiting the roles of nitrogen sources for HEA increment in Cordyceps cicadae

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: May 13, 2024

Cordyceps cicadae, as a new food ingredient, is valuable edible and medicinal fungi. However, its resources are severely depleted due to environmental limitations excessive harvesting practices. N6-(2-hydroxyethyl) adenosine (HEA), an important product of has the potential be used in medical industry diverse disease curing potential. disclosure HEA synthesis still limited application until now. In this study, kinetic curves for under shaker fermentation were explored. The kinetics production exhibited competitive pattern, implicating possibility sharing same step during their synthesis. Due derivative nitrogen metabolism, effect different sources (peptone, yeast extract, ammonium sulfate, diammonium oxalate monohydrate, citrate dibasic, tribasic) on cicadae strain AH 10-4 had been explored incubation conditions (shaker fermentation, stationary submerged fermentation). Our results indicated that complex organic found improve accumulation content fermentation. contrast, optimal source was tribasic. But obviously shortened time comparable capacity by 2.578 mg/g compared with 2.535 mg/g, scaled-up Based dramatic sulfate between fermentations, alanine, aspartate glutamate well arginine metabolic pathway related comparative transcriptome. Further investigation glutamic acid, which analog Asp, showed optimum comparison other amino acids.

Language: Английский

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Evaluation of Lipid Changes During the Drying Process of Cordyceps sinensis by Ultra Performance Liquid Chromatography–Tandem Mass Spectrometry (UPLC-MS/MS)-Based Lipidomics Technique

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(12), P. 855 - 855

Published: Dec. 11, 2024

Comprehensive analysis of the lipid content in

Language: Английский

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HerbMet: Enhancing metabolomics data analysis for accurate identification of Chinese herbal medicines using deep learning

Phytochemical Analysis, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 21, 2024

Chinese herbal medicines have been utilized for thousands of years to prevent and treat diseases. Accurate identification is crucial since their medicinal effects vary between species varieties. Metabolomics a promising approach distinguish herbs. However, current metabolomics data analysis modeling in are limited by small sample sizes, high dimensionality, overfitting.

Language: Английский

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Daily Brain Metabolic Rhythms of Wild Nocturnal Bats

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 9850 - 9850

Published: Sept. 12, 2024

Circadian rhythms are found in a wide range of organisms and have garnered significant research interest the field chronobiology. Under normal circadian function, metabolic regulation is temporally coordinated across tissues behaviors within 24 h period. Metabolites, as closest molecular to physiological phenotype, dynamic patterns their relationship with remains be fully elucidated. In this study, untargeted brain metabolomics was employed investigate daily metabolites at four time points corresponding typical states

Language: Английский

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