Silencing LY6K Suppresses CD44+ EpCAM+ HCT116 Human Colon Cancer Stem Cells Growth: Insights from In Vitro and In Vivo Evidence DOI Creative Commons
Changhao Fu, Kehan Chen,

Jinyue Duan

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(12), P. 14045 - 14057

Published: Dec. 12, 2024

Lymphocyte antigen 6 complex, locus K (LY6K) is a putative oncogene in various human cancers, including colorectal cancer, where elevated expression associated with poor prognosis. This study investigates the antitumor effects of LY6K colon cancer stem cells (CCSCs) both vitro and vivo. EpCAM CD44 surface markers were used to isolate CCSCs from HCT116 cells, was analyzed by real-time PCR. RNA interference silence evaluate its potential role on proliferation, migration, invasion, cell cycle CCSCs. Functional assays, MTS flow cytometric analyses, Transwell migration invasion xenograft model, for analysis. The results revealed that highly expressed siRNA-mediated LY6K-silencing inhibited proliferation inducing G1 phase arrest suppressed invasion. In vivo, silencing effectively reduced tumor growth extended survival mouse model. These findings suggest as promising therapeutic target eradicating treatment.

Language: Английский

Multidimensional transcriptomics based to illuminate the mechanisms of taurine metabolism in immune resistance of pancreatic cancer DOI Creative Commons

Zongshuai Qin,

Gui‐Xiang Huang, Jian Xu

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 31, 2025

Pancreatic cancer, a highly malignant tumor of the digestive system, is characterized by microenvironment with high degree immunosuppression. This immunosuppressive property poses significant challenges, as it hampers effective infiltration immune cells and impairs their ability to exert cytotoxic effects. The metabolic process taurine has emerged crucial factor in modulating functions activities cells. Intervening metabolism holds potential reshape microenvironment, thereby enhancing recognize eliminate To explore therapeutic relationship between disorders pancreatic cancer immunotherapy, we employed multiple software packages, including "Seurat", "DoubletFinder", "Harmony", "GSVA", "CellChat" analyze single-cell data spatial transcriptomic cancer. In present study, four distinct cell subsets, namely RPS4Y1+ cells, LYZ+ CPE+ MKI67+ were identified for first time. CNV score highlighted role within Through cell-communication analysis, crosstalk among fibroblasts, CD8+ T was identified, offering novel insights into immunotherapy strategies, which strengthened co-localization analysis transcriptomics. Furthermore, conducting combined survival data, LY6D target. co-culture experiments uncovered underlying mechanism regulating imbalance establishment "taurine-immune crosstalk" criteria this study effectively paves way immunotherapy. conclusion, current research underscores significance Targeting may represent approach reversing "stiff-cancer" characteristics

Language: Английский

Citations

0
Baicalin Prevents Colon Cancer by Suppressing CDKN2A Protein Expression DOI

Gang-gang Li,

Xiu-feng Chu,

Ya-min Xing

et al.

Chinese Journal of Integrative Medicine, Journal Year: 2024, Volume and Issue: 30(11), P. 1007 - 1017

Published: June 28, 2024

Language: Английский

Citations

1
Application of large-scale and multicohort plasma proteomics data to discover novel causal proteins in gastric cancer DOI Creative Commons
Weihao Tang, Xiaoke Ma

Discover Oncology, Journal Year: 2024, Volume and Issue: 15(1)

Published: Oct. 18, 2024

Gastric cancer (GC) is one of the most common malignant tumors threatening human beings and has a poor prognosis. Therefore, exploring unveiled biomarkers or therapeutic targets for diagnosis treatment GC crucial. A total 5772 protein quantitative trait loci (pQTL) were aggregated from four latest large-scale proteomics cohorts. Two-sample Mendelian randomization (two-sample MR) was utilized to identify causal effect blood plasma proteins on GC. Heterogeneity, pleiotropy, directionality analyses employed evaluate identified via two-sample MR. The robustness results further validated colocalization. drug evaluated reveal compounds that can interfere with these proteins. Ten potential causations in relation identified: LY6D, SLURP1, MLN, PSCA, THSD1, CFTR, PPM1B, KDM3A, TSC1, HCG22. Among proteins, THSD1 considered as reliable due their significant H4 posterior probabilities colocalization analysis absence pleiotropy. Compound 35, nitrosamide, 0175029-0000 drugs small molecules targeting respectively. This study several provided data support new insights into early diagnosis, intervention,

Language: Английский

Citations

0
Silencing LY6K Suppresses CD44+ EpCAM+ HCT116 Human Colon Cancer Stem Cells Growth: Insights from In Vitro and In Vivo Evidence DOI Creative Commons
Changhao Fu, Kehan Chen,

Jinyue Duan

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(12), P. 14045 - 14057

Published: Dec. 12, 2024

Lymphocyte antigen 6 complex, locus K (LY6K) is a putative oncogene in various human cancers, including colorectal cancer, where elevated expression associated with poor prognosis. This study investigates the antitumor effects of LY6K colon cancer stem cells (CCSCs) both vitro and vivo. EpCAM CD44 surface markers were used to isolate CCSCs from HCT116 cells, was analyzed by real-time PCR. RNA interference silence evaluate its potential role on proliferation, migration, invasion, cell cycle CCSCs. Functional assays, MTS flow cytometric analyses, Transwell migration invasion xenograft model, for analysis. The results revealed that highly expressed siRNA-mediated LY6K-silencing inhibited proliferation inducing G1 phase arrest suppressed invasion. In vivo, silencing effectively reduced tumor growth extended survival mouse model. These findings suggest as promising therapeutic target eradicating treatment.

Language: Английский

Citations

0