Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Nov. 26, 2024
Abstract
A
novel
modified
sensor
based
on
electropolymerization
of
hippuric
acid
(HA)
using
cyclic
voltammetry
within
the
potential
window
−
1
to
1.5
V
for
10
cycles
at
a
scan
rate
100
mV
s
−1
over
multiwalled
carbon
nanotubes
(MWCNTs)
battery
graphite
electrode
(BGE).
Poly
(HA)/MWCNTs/BGE
exhibited
two
linearity
ranges
3.00
×
−3
1.00
µM
(5.29
−4
–
0.18
µg/ml)
and
5.00
3
(0.88−
176.22
with
limit
detection
(LOD)
0.06
−2
(1.06
quantification
(LOQ)
2.00
(3.52
µg/ml).
The
poly
was
successfully
applied
determination
SER
in
presence
tryptophan
human
blood
serum
recovery
98.31–105.47%
RSD
values
3.02–
4.77%.
Green
chemistry
metrics
:
national
environmental
index
(NEMI),
analytical
greenness
metric
(AGREE),
Raynie
Driver,
green
procedure
(GAPI),
eco-scale
were
employed
indicated
that
proposed
can
be
classified
as
an
excellent
method,
achieving
score
84.
Frontiers in Immunology,
Journal Year:
2025,
Volume and Issue:
16
Published: Feb. 19, 2025
The
thymus
is
a
primary
lymphoid
organ
critical
for
the
development
of
mature
T
cells
from
hematopoietic
progenitors.
A
highly
structured
organ,
contains
distinct
regions,
precise
cytoarchitecture,
and
molecular
signals
tightly
regulating
thymopoiesis.
Although
above
are
well-understood,
structural
functional
implications
thymic
innervation
largely
neglected.
In
general,
neural
regulation
has
become
increasingly
identified
as
component
immune
cell
function.
central
nervous
system
(CNS)
in
brain
coordinates
these
immunological
responses
both
by
direct
through
peripheral
nerves
neuroendocrine
signaling.
Yet
how
signals,
particularly
innervation,
may
regulate
biology
unclear
understudied.
this
review,
we
highlight
historical
current
data
demonstrating
input
to
assess
evidence
We
further
discuss
knowledge
gaps
summarize
recent
advances
techniques
that
could
be
used
study
microenvironment.
RSC Advances,
Journal Year:
2024,
Volume and Issue:
14(38), P. 27999 - 28016
Published: Jan. 1, 2024
Fabrication
of
a
sensitive
electrochemical
sensor
based
on
the
composite
reduced
graphene
oxide
and
multiwalled
carbon
nanotubes
modified
glassy
electrode
for
simultaneous
measurements
hydroquinone,
dopamine,
uric
acid.
Heliyon,
Journal Year:
2025,
Volume and Issue:
11(4), P. e42690 - e42690
Published: Feb. 1, 2025
Experimental
designs
are
essential
mathematical
tools
in
fields
like
agronomy,
chemistry,
and
analytical
chemistry
for
optimizing
processes
minimizing
variations.
Doehlert
designs,
particular,
valued
their
efficiency
exploring
experimental
space
with
minimal
experiments,
providing
detailed
insights
into
complex
processes.
In
these
extensively
used
tasks
such
as
extraction,
purification,
method
optimization,
allowing
systematic
variation
of
factors
to
enhance
accuracy
efficiency.
To
further
optimize
the
method,
a
combination
design
chemometric
is
necessary
understand
chromatographic
behavior
compounds
interest.
This
study
uses
combined
elution
separation
neurotransmitters
metabolites
using
HPLC-ECD.
Pearson
correlation
Partial
Least
Squares
Discriminant
Analysis
(PLS-DA)
reveal
significant
relationships
between
parameters
conditions.
Notably,
pH
mobile
phase
significantly
impacts
column
time,
while
polarity
index
pressure
influence
peak
asymmetry.
Optimized
conditions
include
ACN/MeOH/H2O
(11.25/3.25/85.5;
v/v/v)
near
1.65,
achieving
optimal
times
around
20
min,
mean
number
theoretical
plates
close
8000,
asymmetry
approximately
1.23.
The
limits
detection
quantification
interest
10-11
10-10
mol
L-1
respectively.
new
approach
allows
effective,
rapid,
resolved
compounds,
reducing
resource
consumption
time.
Moreover,
has
been
taken
account
chemometrics,
highly
effective
enhancement
compound
elution.
optimized
achieves
low
nanomolar
range,
making
it
suitable
precise
neurotransmitter
analysis
biological
samples.
Asian Journal of Pharmaceutical and Clinical Research,
Journal Year:
2025,
Volume and Issue:
unknown, P. 221 - 231
Published: May 7, 2025
Objective:
Neuropathic
pain
(NP)
arises
from
trauma
to
the
somatosensory
nervous
system
and
can
be
managed
using
selective
serotonin
reuptake
inhibitors,
such
as
fluoxetine
(FLX)
phosphodiesterase
sildenafil
(SD),
cholesterol-lowering
agents
lovastatin
(LOVA).
The
present
study
aimed
develop
validate
an
analytical
method
for
simultaneous
estimation
of
these
drugs
(SD,
FLX,
LOVA
[SFL])
reverse-phase
high-performance
liquid
chromatography
(RP-HPLC).
Methods:
An
RP-HPLC
was
developed
validated
quantification
SFL.
Chromatographic
separation
achieved
a
C-18
ODS
column
with
mobile
phase
consisting
acetonitrile
0.2
M
ammonium
acetate
buffer
(55:45)
in
gradient
elution
mode.
flow
rate
maintained
at
1
mL/min,
detection
carried
out
228
nm.
following
ICH
Q2
(R2)
guidelines,
assessing
parameters
linearity,
accuracy,
precision,
limit
(LOD),
(LOQ).
Results:
exhibited
linearity
within
concentration
range
20–100
μg/mL,
regression
coefficient
(r2)
0.9992.
Retention
times
SD,
were
recorded
6.481,
4.238,
19.778
min,
respectively.
Recovery
studies
demonstrated
accuracy
94.61–110.44%,
relative
standard
deviation
0.06–2.00%,
confirming
precision
method.
LOD
values
found
12.77
14.81
13.28
respectively,
while
LOQ
45.16
42.33
38.71
μg/mL.
Conclusion:
met
all
required
validation
criteria
suitability
accurate
pharmaceutical
formulations.
These
findings
support
potential
use
alternative
therapeutic
strategy
NP.
