Enhanced performance of bacterial laccase via microbial surface display and biomineralization for portable detection of phenolic pollutants

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: unknown, P. 137957 - 137957

Published: March 1, 2025

Language: Английский

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Innovations in packed-bed reactors utilizing immobilized lipase catalysts: A comprehensive technical and scientific review

Molecular Catalysis, Journal Year: 2025, Volume and Issue: 573, P. 114814 - 114814

Published: Jan. 5, 2025

Language: Английский

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Immobilization of Trametes versicolor laccase on polystyrene microspheres containing diazonium groups

Polymer Bulletin, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Language: Английский

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Biocatalytic Potential of a Raoultella terrigena-Derived Lipolytic Enzyme for High-Performance Detergents

Fermentation, Journal Year: 2025, Volume and Issue: 11(4), P. 225 - 225

Published: April 17, 2025

Dump sites harbour microorganisms with potential for environmentally friendly industrial applications. This study assessed the lipolytic activity of municipal dumpsite-associated bacteria and evaluated stability most potent isolate’s enzyme against laundry detergents. It also examined crude lipase’s ability to remove stains from cotton fabric. Among twelve isolated, five demonstrated notable halo zones on tributyrin agar plates. The diameters (mm) were MN38 (11 ± 1.4), MN1310 (8.5 0.7), MN28 (6.5 0.71), MN18 (7.0 MN310 (8.15 0.21). Quantitative analysis revealed that exhibited highest lipase (14.76 0.27 U/mL), while showed lowest (6.40 0.85 U/mL). Nucleotide sequence identified isolates as Raoultella terrigena veli18 (MN38), Stenotrophomonas maltophilia veli96 (MN1310), Viridibacillus sp. veli10 (MN28), veli19 (MN18), Klebsiella veli70 (MN310). R. maintained 73.33%, 52.67%, 55.0%, 54.0% its original after 60 min exposure Sunlight, Surf, Maq, Omo, respectively. Adding enzyme-free detergents significantly enhanced their cleaning efficacy, completely removing oil performance highlights an effective detergent bio-additive.

Language: Английский

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Enhancement of Enzyme Activity by Alternating Magnetic Field and Near-Infrared Irradiation

Catalysts, Journal Year: 2025, Volume and Issue: 15(4), P. 386 - 386

Published: April 16, 2025

The enhancement of enzyme activity has garnered significant attention in biotransformation processes and applications. This is achieved through the use specific nanomaterials (NMs) with unique physicochemical characteristics responsive to external stimuli. In this study, an enzyme–Fe3O4 nano-biocatalytic system (NBS) was developed enable real-time activation enzymatic catalysis under alternating magnetic field (AMF) near-infrared (NIR) irradiation using dual-functional Fe3O4 nanoparticles (MNPs). When exposed AMF, MNPs generate molecular vibrations mechanisms such as Néel or Brown relaxation while acting a photothermal agent response NIR irradiation. synergistic effect AMF significantly enhanced energy transfer between MNPs, resulting maximum 4.3-fold increase activity. Furthermore, reduced aldol reaction time by 66% (from 4 h 1.5 h) achieving 90% product yield. Additionally, factors nanoparticle size power were found play critical role efficiency regulation strategy. results also demonstrate that nanocomposites (NCs) catalytic for reactions. study demonstrates efficient NBS controlled via effects irradiation, enabling spatiotemporal control biochemical work provides breakthrough strategy dynamic biocatalysis, potential applications industrial biomanufacturing, on-demand drug synthesis, precision nanomedicine.

Language: Английский

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Active Site Studies to Explain Kinetics of Lipases in Organic Solvents Using Molecular Dynamics Simulations

The Journal of Physical Chemistry B, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 29, 2024

This study investigates the intricate dynamics underlying lipase performance in organic solvents using comprehensive molecular (MD) simulations, supported by enzyme kinetics data. The reveals that a single criterion can neither predict nor explain activity solvents, indicating need for approach. Three lipases were included this study:

Language: Английский

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Laccase: exploring structural insights and functional versatility for applications

Biologia, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 3, 2024

Language: Английский

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Investigating PCB degradation by indigenous fungal strains isolated from the transformer oil–contaminated site: degradation kinetics, Bayesian network, artificial neural networks, QSAR with DFT, molecular docking, and molecular dynamics simulation

Environmental Science and Pollution Research, Journal Year: 2024, Volume and Issue: 31(43), P. 55676 - 55694

Published: Sept. 6, 2024

Language: Английский

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Simultaneous Degradation of AFB1 and ZEN by CotA Laccase from Bacillus subtilis ZJ-2019-1 in the Mediator-Assisted or Immobilization System

Toxins, Journal Year: 2024, Volume and Issue: 16(10), P. 445 - 445

Published: Oct. 16, 2024

The global prevalence of aflatoxin B1 (AFB1) and zearalenone (ZEN) contamination in food feed poses a serious health risk to humans animals. Recently, enzymatic detoxification has received increasing attention, yet most enzymes are limited degrading only one type mycotoxin, free often exhibit reduced stability activity, limiting their practicality real-world applications. In this study, the laccase

Language: Английский

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Immobilization of Trametes versicolor laccase on polystyrene microspheres containing diazonium groups

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

Laccases are versatile enzymes employed in the degradation of pollutants and organic synthesis reactions. Their immobilization enables reuse these biocatalysts, thereby reducing overall process costs. However, studies on laccases onto polymers containing diazonium groups remain limited literature. This work investigates relationship between experimental parameters—namely pH, ionic strength, contact time enzyme solution diazonium-functionalized polystyrene—and their effects yield biocatalyst activity oxidation 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). A five-level design was employed, varying strength citrate-phosphate buffer, time. The correlation reaction variables response best fitted using second-order models. pH its quadratic interaction significantly affected yield, while were significant determining resulting biocatalysts. validity proposed regression models confirmed by adjusted R² values, F significance, residual analysis. After two, three, four, five, six cycles, catalytic losses 33%, 46%, 50%, 59%, 51% initial activity, respectively. loss cycles low, suggesting that material is suitable for extended reuse. Furthermore, after 120 days storage, retained 89.9% relative indicating good stability when stored under refrigeration.

Language: Английский

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