Depolymerization of oyster glycosaminoglycans for the enhancement of α-glucosidase inhibition and its application in hypoglycemic studies

Food Research International, Journal Year: 2025, Volume and Issue: 205, P. 116008 - 116008

Published: Feb. 14, 2025

Language: Английский

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Phenylthiourea synergistically enhances the hepatotoxicity of bavachalcone by interfering with metabolism in zebrafish: A factor to consider when evaluating toxicity of environmental pollutants using zebrafish models

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 292, P. 118016 - 118016

Published: March 1, 2025

Phenylthiourea (PTU) is a well-known inhibitor of melanin synthesis that has been extensively utilized in ecotoxicological studies involving zebrafish. Although there are reports suggesting PTU may influence the toxicity various compounds, underlying mechanisms its action remain unclear. Bavachalcone (BavaC) wide range applications agriculture and medicine, it can enter groundwater through variety pathways pose risk to aquatic ecosystems. We found enhanced hepatotoxicity BavaC zebrafish, but mechanism was In this study, interactive effects 200 μM 2.5 on zebrafish larvae were evaluated after 72 h exposure. significantly increased BavaC-induced hepatotoxicity, which characterized by liver hypoplasia, hepatocyte vacuolation, lipid accumulation. Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) revealed contents fatty acids, phosphatidylcholine glutathione increased. The results RNA sequencing (RNA-seq) Real-time PCR (RT-qPCR) analyses indicated several metabolic pathways, including xenobiotic biodegradation metabolism, amino acid metabolism carbohydrate differentially regulated PTU+BavaC group compared group. Our findings indicate PTU-induced disorders exacerbate highlight need reconsider use zebrafish-based assessments environmental pollutants.

Language: Английский

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The mechanism of maternal inheritance of glycolipid metabolism disorder in a zebrafish model of type 2 diabetes

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 30, 2025

In recent years, the number of people with type 2 diabetes mellitus (T2DM) has been increasing, and there is obvious familial aggregation T2DM. Pregestational gestational are most common chronic conditions during pregnancy. However, mechanism by which maternal preconception hyperglycaemia affects glucolipid metabolism in offspring not fully understood. Zebrafish have widely used to construct T2DM models. this study, we established a successful model female zebrafish immersing them 2% glucose solution for 28 days. The results showed that group exhibited damage ovaries livers. Fasting blood glucose, insulin, cholesterol triglycerides were increased compared control group. Moreover, delayed hatching rate, yolk sac area, body length heart rate decreased flow velocity observed F1 larvae from Glucose, insulin lipid prominently affected offspring. Importantly, influence on fasting could persist into adulthood Transcriptomic indicated signalling pathways gluconeogenesis, fat digestion absorption amino acid enriched perturbed This study emphasised impacts glycolipid offspring, transferring origin disease stage providing reference further research aetiology disorders.

Language: Английский

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Zebrafish navigating the metabolic maze: insights into human disease – assets, challenges and future implications

Journal of Diabetes & Metabolic Disorders, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 16, 2024

Language: Английский

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