Optimization of the suspension procedure by Box-Behnken design for the determination of manganese, iron, and zinc in zeolite clinoptilolite with the TXRF system and insight into its antioxidant properties DOI Creative Commons
Jasna Jablan,

Maja Bival Štefan,

Dario Paler

et al.

Acta Pharmaceutica, Journal Year: 2024, Volume and Issue: 74(4), P. 655 - 672

Published: Dec. 1, 2024

Abstract Zeolites are a large family of minerals and the most studied is naturally occurring clinoptilolite. They possess anti-inflammatory, antioxidant, detoxifying properties which makes them valuable for medicinal use. Element analysis zeolite’s composition necessary its precise chemical characterization, within this work development suspension method determination manga nese, iron, zinc by total reflection X-ray fluorescence spec-trometry (TXRF) was presented. The Box-Behnken design based on response surface methodology applied to determine optimal sample preparation conditions. significant variables such as amount, volume deposition, dispersant were selected critical variables. Based results obtained, suspensions prepared weighing 10 mg adding 1 mL 5 % Triton X-100 with Ga internal standard deposition set at mL. obtained TXRF comparable those FAAS method, indicating that technique can be used instead conventional methods. Using best analytical conditions, limits detection trace elements in range 0.2–0.6 kg –1 . Trueness precision results, evaluated CRM analysis, cases acceptable recoveries values 104.9–111.4 relative deviations 2–10 (. = 6). showed no ability quench free radicals nor influence dietary antioxidants.

Language: Английский

Lactoferrin/lactoferrin receptor: Neurodegenerative or neuroprotective in Parkinson’s disease? DOI
Zhong‐Ming Qian, Wei Li,

Qian Guo

et al.

Ageing Research Reviews, Journal Year: 2024, Volume and Issue: 101, P. 102474 - 102474

Published: Aug. 27, 2024

Language: Английский

Citations

4
Targeting Ferroptosis/Nrf2 Pathway Ameliorates AlCl3-Induced Alzheimer’s Disease in Rats: Neuroprotective Effect of Morin Hydrate, Zeolite Clinoptilolite, and Physical Plus Mental Activities DOI Open Access
Karema Abu-Elfotuh,

Yasmin Mahran,

Walaa Bayoumie El Gazzar

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1260 - 1260

Published: Jan. 31, 2025

Alzheimer’s disease (AD) is a significant health challenge in the 21st century. In spite of approval many new disease-modifying therapies for AD, clinical advantages these treatments are less certain. Aim: This investigation was intended to determine potential neuroprotective impact morin hydrate (MH), zeolite clinoptilolite (ZC), and/or physical and mental activities (PhM) on an aluminum chloride (AlCl3)-induced AD rat model. Methods: Male Sprague Dawley rats were randomly allocated into seven groups. Group I control group. Groups II–VII treated with AlCl3 5 weeks. III–VII tested effects MH, ZC, PhM. Biochemical, brain histopathological, behavioral studies performed. Results: PhM, ZC combined therapy exhibited effect demonstrated by corrected catecholamines tau β-amyloid levels, as well antioxidant anti-ferroptotic probably through Nrf2/HO-1/GPX4 ACSL4 signaling pathways. addition, counteracted inflammatory responses modulating TLR4/NF-κβ/NLRP3 inflammasome expression. Moreover, groups showed maximum improvement both APOE4/LRP1 Wnt3/β-catenin/GSK-3β expressions. Conclusion: research highlights MH plus PhM against AlCl3-induced via modulation Nrf2/HO-1/GPX4, TLR4/NF-κβ/NLRP3, APOE4/LRP1, It first point out inclusion ferroptosis-Nrf2/inflammasomes cross-talk neuroprotection mechanism MH/ZC AlCl3-mediated

Language: Английский

Citations

0
Oral Supplementation with Modified Natural Clinoptilolite Protects Against Cadmium Toxicity in ICR (CD-1) Mice DOI Creative Commons

Michaela Beltcheva,

Yana Tzvetanova, Peter Ostoich

et al.

Toxics, Journal Year: 2025, Volume and Issue: 13(5), P. 350 - 350

Published: April 27, 2025

For the first time, this study investigates in vivo potential of Na-modified natural clinoptilolite to mitigate cadmium toxicity ICR mice, a model relevant human health. We enhanced improve its (Cd) exchange capacity. Mice were exposed environmentally realistic nitrate Cd(NO3)2 doses their drinking water. The detoxification efficacy mineral was evaluated over 45 days four groups: control (no supplementation), only, and combination clinoptilolite. assessed Cd bioaccumulation liver kidneys, genotoxicity (micronucleus assay), hematological parameters, oxidative stress markers. exposure resulted significant bioaccumulation, reduced growth, changes erythrograms, DNA damage, stress. receiving alone showed increase body mass. Modified led nearly 48% reduction accumulation 30% excretion Cd-plus-clinoptilolite group compared Cd-only group. Erythrogram leukogram parameters returned near-normal levels, with reductions malondialdehyde (MDA) increases glutathione (GSH) observed by end experiment. No elevated levels micronuclei found following supplementation. These results suggest that modified may be cost-effective detoxifier Cd-polluted regions.

Language: Английский

Citations

0
Optimization of the suspension procedure by Box-Behnken design for the determination of manganese, iron, and zinc in zeolite clinoptilolite with the TXRF system and insight into its antioxidant properties DOI Creative Commons
Jasna Jablan,

Maja Bival Štefan,

Dario Paler

et al.

Acta Pharmaceutica, Journal Year: 2024, Volume and Issue: 74(4), P. 655 - 672

Published: Dec. 1, 2024

Abstract Zeolites are a large family of minerals and the most studied is naturally occurring clinoptilolite. They possess anti-inflammatory, antioxidant, detoxifying properties which makes them valuable for medicinal use. Element analysis zeolite’s composition necessary its precise chemical characterization, within this work development suspension method determination manga nese, iron, zinc by total reflection X-ray fluorescence spec-trometry (TXRF) was presented. The Box-Behnken design based on response surface methodology applied to determine optimal sample preparation conditions. significant variables such as amount, volume deposition, dispersant were selected critical variables. Based results obtained, suspensions prepared weighing 10 mg adding 1 mL 5 % Triton X-100 with Ga internal standard deposition set at mL. obtained TXRF comparable those FAAS method, indicating that technique can be used instead conventional methods. Using best analytical conditions, limits detection trace elements in range 0.2–0.6 kg –1 . Trueness precision results, evaluated CRM analysis, cases acceptable recoveries values 104.9–111.4 relative deviations 2–10 (. = 6). showed no ability quench free radicals nor influence dietary antioxidants.

Language: Английский

Citations

0