Talanta, Journal Year: 2025, Volume and Issue: 294, P. 128236 - 128236
Published: May 3, 2025
Language: Английский
Talanta, Journal Year: 2025, Volume and Issue: 294, P. 128236 - 128236
Published: May 3, 2025
Language: Английский
Biosensors, Journal Year: 2025, Volume and Issue: 15(2), P. 65 - 65
Published: Jan. 21, 2025
Vitamin B12 (VB12) is an important nutrient, and its quality control in food crucial. In this study, based on the principle of specific recognition target analyte by monoclonal antibodies (mAbs), a time-resolved fluorescent microsphere immunochromatographic assay (TRFM-ICA) was developed to detect content VB12 infant formula milk powder. First, performance anti-VB12 mAb evaluated, revealing half-maximal inhibitory concentration 0.370 ng/mL, affinity constant 2.604 × 109 L/mol no cross-reactivity with other vitamins. Then, highly sensitive TRFM-ICA developed, visual limit detection 10 μg/kg cut-off value 100 for qualitative range 4.125–82.397 quantitative detection. addition, test results real samples were consistent quantification using microbiological methods, coefficient variation less than 10%, showing good accuracy stability, confirming that suitable analysis powder samples. (mAbs) against VB12, fluorescence converting biological signals into optical signals.
Language: Английский
Citations
0Sensors and Actuators Reports, Journal Year: 2025, Volume and Issue: unknown, P. 100291 - 100291
Published: Jan. 1, 2025
Language: Английский
Citations
0Published: Jan. 1, 2025
Language: Английский
Citations
0Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113338 - 113338
Published: March 1, 2025
Language: Английский
Citations
0TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118277 - 118277
Published: April 1, 2025
Language: Английский
Citations
0Talanta, Journal Year: 2025, Volume and Issue: 294, P. 128236 - 128236
Published: May 3, 2025
Language: Английский
Citations
0