Chemical Profiling, Antioxidant and Antimicrobial Activities, and In Silico Evaluation of Gardenia jasminoides Essential Oil

Plants, Journal Year: 2025, Volume and Issue: 14(7), P. 1055 - 1055

Published: March 28, 2025

Aromatic and medicinal plants have been integral to human civilization for thousands of years, serving not only as vital components in traditional modern medicine but also sources captivating fragrances that enhance our sensory experiences. The main objective this study was explore the chemical composition, antioxidant antimicrobial properties, silico molecular docking attributes Gardenia jasminoides essential oil (GJEO). compositions were determined using gas chromatography-mass spectrometry (GC-MS) analysis. activity by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) total capacity (TAC) test. tested vitro three microbial strains (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus), two fungal (Candida albicans Aspergillus niger). In analysis used determine interaction types topoisomerase II receptors most important compounds (Eugenol, Methyleugenol, α-Terpineol ligands). obtained results highlight presence 25 volatile including 5 new detected compounds: Methyleugenol (15.41%), 1-Undecyne (3.4%), 2,6,10-Dodecatrien-1-ol, 3,7,11-trimethyl- (1.11%), 2,5-Cyclohexadiene-1,4-dione, 2,6-bis(1,1-dimethylethyl)- (0.4%), 5,9-Tetradecadiyne (0.32%). GJEO is around 1.25 µg equivalent ascorbic acid/mL TAC assay IC50 = 19.05 µL/mL DPPH exhibited significant activity, particularly against with a minimum inhibitory concentration (MIC) 16.67 µL/mL. revealed strong interactions between ethyleugenol characterized multiple bonding interactions, Pi-Alkyl carbon-hydrogen bonds, while formed hydrogen alkyl interactions. These underline potential promising source bioactive highlighting its possible applications pharmaceuticals natural therapies.

Language: Английский

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Dietary quorum quenching AHL lactonase impairs the adaptation of commensal bacterium to host innate immunity

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Abstract Quorum sensing (QS) is the communication system of bacteria that depends on QS signals. quenching (QQ) enzymes degrade signals and are promising alternatives antibiotics to treat bacterial infections. Here, we found dietary QQ N -acyl homoserine (AHL) lactonase led microbiota dysbiosis in zebrafish, with reduction Aeromonas enrichment Plesiomonas . Through gnotobiotic zebrafish colonized a minimal microbiota, QQ-mediated microbial alteration relies host Myd88 signaling neutrophil elastase. Mechanistically, quorum increased susceptibility commensal elastase by impairing lateral flagellar system, leading reduced colonization subsequent due ecological competition two species. Together, adaptation innate immunity, which provided novel insight role host-microbiota interaction.

Language: Английский

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Targeting the quorum sensing network in Acinetobacter baumannii: A dual target structure-based approach for the development of novel antimicrobials

Computers in Biology and Medicine, Journal Year: 2025, Volume and Issue: 187, P. 109828 - 109828

Published: Feb. 11, 2025

Language: Английский

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Molecular structural arrangement in quorum sensing and bacterial metabolic production

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(2)

Published: Feb. 1, 2025

Language: Английский

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Two amino-substituted Diphenyl Fumaramide Derivatives inhibit the virulence regulated by quorum sensing system of Pseudomonas aeruginosa

Journal of Applied Microbiology, Journal Year: 2025, Volume and Issue: 136(3)

Published: Feb. 19, 2025

Abstract Aim Pseudomonas aeruginosa employs the quorum sensing (QS) system, a sophisticated cell-to-cell communication mechanism, to modulate synthesis and secretion of range virulence factors, which contribute establishment acute or chronic infections in hosts. This study seeks attenuate P. by inhibiting QS thereby reducing its pathogenicity as promising alternative traditional antibiotics. Methods results Two compounds with an amino-substituted diphenyl fumaramide core, N1-(4-bromophenyl)-N4-(4′-oxo-3′,4′-dihydro-1′H-spiro [cyclopentane-1,2′-quinazolin]-6′-yl) (10D) N1-(3-chloro-4-fluorophenyl)-N4-(4-oxo-3,4,4′,5′-tetrahydro-1H,2′H-spiro [quinazoline-2,3′-thiophen]-6-yl) (12A), were identified through in-silico screening. The inhibitory potential both was explored vitro vivo. In experiments, neither compound exhibited bactericidal effects but significantly inhibited production QS-regulated extracellular protease pyocyanin. Quantitative PCR analysis revealed that QS-activated genes downstream transcriptionally suppressed 10D 12A. Molecular docking molecular dynamics simulations predicted stable interactions between these key regulators LasR PqsR. When combined polymyxin B, kanamycin, levofloxacin, 12A synergistic antibacterial activity. Furthermore, improved survival mice challenged effectively reduced bacterial load lungs. Conclusion indicates possess considerable potential, attenuating aeruginosa. Moreover, offers structural insights methodological guidance for advancement anti-virulence drug development.

Language: Английский

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Multidrug-resistant Serratia marcescens: A growing threat in Iraqi intensive care units

Gene Reports, Journal Year: 2025, Volume and Issue: unknown, P. 102197 - 102197

Published: March 1, 2025

Language: Английский

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Understanding the Role of Bacterial Biofilm in Antibiotic Resistance: Defensive Strategies and Clinical Challenges

IntechOpen eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Bacterial biofilms significantly cause persistent exacerbation of infections in the clinical setting. These groups microorganisms are highly resistant to host immune responses and conventional antibiotic therapies, whereas they embedded an extracellular matrix. This chapter provides more detailed information on mechanism biofilm formation involving key stages adherence, maturation, spread, including composition structure a further explores how contribute resistance, physical barriers drug penetration, quorum sensing mechanisms, adaptive resistance strategies such as genetic adaptation, stress responses, persister cells. The role horizontal gene transfer spread within communities is also discussed. discusses challenges posed by biofilm-associated infections, focusing diagnosing treating chronic recurrent factors persistence, limitations current therapeutic options. Finally, we address emerging countermeasures counter mediated biofilms, enzymatic nanomedicine technologies, natural product-based inhibitors, inhibitory agents, photodynamic sonodynamic antimicrobial therapy, combinatorial therapies.

Language: Английский

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Innovative Strategies to Combat Antibiotic Resistance: Emerging Trends and Future Directions

International Journal of Health Management and Tourism, Journal Year: 2025, Volume and Issue: 10(1), P. 75 - 105

Published: March 27, 2025

Aim: Antimicrobial resistance (AMR) represents a critical global health challenge exacerbated by the overuse and misuse of antibiotics in human, animal, environmental contexts. This study aims to examine barriers addressing AMR, with specific focus on scientific, economic, regulatory challenges development adoption novel antimicrobial strategies. Methods: review synthesizes current literature innovative therapeutic approaches, such as bacteriophage therapy peptides, alongside an analysis policy initiatives, including WHO Global Action Plan AMR PASTEUR Act. Emphasis is placed identifying key obstacles potential solutions within realms antibiotic R&D frameworks. Results: Findings highlight significant promise alternative therapies policy-driven incentives address AMR. However, limitations scientific hurdles, economic disincentives, disparities enforcement hinder progress. Novel measures like subscription-based models improved diagnostic tools have shown close existing gaps. Conclusion: Collaborative efforts are essential effectively. Sustainable funding mechanisms, advanced technologies, integrated One Health approaches must be prioritized bridge gaps between science, policy, practice. By these challenges, community can mitigate escalating threat safeguard efficacy for future generations. Keywords: resistance, development, health, Health, initiatives

Language: Английский

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Influence of Microbiome Interactions on Antibiotic Resistance Development in the ICU Environment: Insights and Opportunities with Machine Learning

Deleted Journal, Journal Year: 2025, Volume and Issue: 70(2), P. 14 - 14

Published: April 9, 2025

Antibiotic resistance is a global health crisis exacerbated by the misuse of antibiotics in healthcare, agriculture, and environment. In an intensive care unit (ICU), where high antibiotic usage, invasive procedures, immunocompromised patients converge, risks are amplified, leading to multidrug-resistant organisms (MDROs) poor patient outcomes. The human microbiome plays crucial role development dissemination genes (ARGs) through mechanisms like horizontal gene transfer, biofilm formation, quorum sensing. Disruptions balance, or dysbiosis, further exacerbate resistance, particularly high-risk ICU environments. This study explores interactions ICU, highlighting machine learning (ML) as transformative tool. Machine algorithms analyze high-dimensional data, predict patterns, identify novel therapeutic targets. By integrating genomic, microbiome, clinical these models support personalized treatment strategies enhance infection control measures. results demonstrate potential improve stewardship outcomes, emphasizing its utility ICU-specific interventions. conclusion, addressing requires multidisciplinary approach combining advanced computational methods, research, expertise. Enhanced surveillance, targeted interventions, collaboration essential mitigate care.

Language: Английский

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Dihydromyricetin alleviates ETEC K88-induced intestinal inflammatory injury by inhibiting quorum sensing-related virulence factors

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 9, 2025

Language: Английский

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