Multi-omics analysis reveals lysosome-associated molecular subtype characterization and prognostic modeling system in lung adenocarcinoma

Journal of Cancer, Journal Year: 2025, Volume and Issue: 16(6), P. 1794 - 1813

Published: Feb. 18, 2025

Background: Lung adenocarcinoma (LUAD) poses a significant challenge in current treatments due to its high recurrence and metastasis rates. Despite preliminary evidence suggesting the role of lysosomes LUAD, it remains unclear whether lysosome-related functions can be effectively used for risk stratification LUAD patients involved functional targets are still needed explored. Method: An integrated analysis TCGA GEO databases was conducted explore potential genes (LRGs) LUAD. Unsupervised consensus clustering utilized LRG molecular subtypes ESTIMATE ssGSEA algorithms were performed evaluate immune infiltration characterization samples. LASSO-univariate multivariate Cox construct score model. Single-cell sequencing reveal distribution characteristics different cell subpopulations selected LRGs. In vitro experiments including western blotting, PCR, colony formation assays, Transwell assays verify expression biological target Results: Through multi-omics integration algorithms, we successfully developed prognostic system based on scoring constructed nomogram diagnostic Various bioinformatics analyses indicated clinical value system. further revealed composition signatures. SLC2A1, one targets, validated through regulate proliferation migration cells, thereby confirming reliability results. Conclusion: Our results demonstrate that effective achieved LRGs by integration. Furthermore, key vitro, providing new ideas future treatment.

Language: Английский

Alzheimer’s, Parkinson’s, Frontotemporal Lobar Degeneration, and Amyotrophic Lateral Sclerosis Start in Pediatric Ages: Ultrafine Particulate Matter and Industrial Nanoparticles Are Key in the Early-Onset Neurodegeneration: Time to Invest in Preventive Medicine

Toxics, Journal Year: 2025, Volume and Issue: 13(3), P. 178 - 178

Published: Feb. 28, 2025

Billions of people are exposed to fine particulate matter (PM2.5) levels above the USEPA’s annual standard 9 μg/m3. Common emission sources anthropogenic, producing complex aerosolized toxins. Ultrafine (UFPM) and industrial nanoparticles (NPs) have major detrimental effects on brain, but USA does not measure UFPM a routine basis. This review focuses development progression common neurodegenerative diseases, as diagnosed through neuropathology, among young residents in Metropolitan Mexico City (MMC). MMC is one most polluted megacities world, with population 22 million residents, many whom unaware brain caused by their atmosphere. Fatal diseases (such Alzheimer’s Parkinson’s) that begin childhood populations living air environments preventable. We conclude UFPM/NPs capable disrupting neural homeostasis give rise relentless processes throughout entire life highly MMC. The paradigm reaching old age neurodegeneration no longer supported. Neurodegenerative changes start early pediatric ages irreversible. It time invest preventive medicine.

Language: Английский

Innovative Nanomedicine Delivery: Targeting Tumor Microenvironment to Defeat Drug Resistance

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(12), P. 1549 - 1549

Published: Dec. 3, 2024

Nanodrug delivery systems have revolutionized tumor therapy like never before. By overcoming the complexity of microenvironment (TME) and bypassing drug resistance mechanisms, nanotechnology has shown great potential to improve efficacy reduce toxic side effects. This review examines impact TME on recent advances in nanomedicine overcome this challenge. Characteristics such as hypoxia, acidity, high interstitial pressure significantly effectiveness chemotherapy radiotherapy, leading increased cells. Then, summarizes innovative nanocarrier designs for these microenvironmental features, including hypoxia-sensitive nanoparticles, pH-responsive carriers, multifunctional nanosystems that enable targeted release improved penetration accumulation tumors. combining with therapeutic strategies, offers a novel perspective by focusing design nanocarriers interact TME, dimension often overlooked similar reviews. We highlight dual role modulation, emphasize their resistance, look at future research directions.

Language: Английский