Nanolabels Prepared by the Entrapment or Self-Assembly of Signaling Molecules for Colorimetric and Fluorescent Immunoassays DOI Creative Commons
Ning Xia,

Y.-C. Li,

Cancan He

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(12), P. 597 - 597

Published: Dec. 6, 2024

Nanomaterials have attracted significant attention as signal reporters for immunoassays. They can directly generate detectable signals or release a large number of signaling elements readout. Among various nanolabels, nanomaterials composed multiple molecules shown great potential in Generally, be entrapped nanocontainers self-assemble into nanostructures amplification. In this review, we summarize the advances molecules-entrapped assembled colorimetric and fluorescence The cover liposomes, polymers, mesoporous silica, metal–organic frameworks (MOFs), nanosheets, nanoflowers nanocages, etc. Signaling mainly refer to visible and/or fluorescent organic dyes. design application immunoassays are emphasized from perspective nanocontainers, analytes, analytical performances. addition, future challenges research trends preparation nanolabels briefly discussed.

Language: Английский

Establishment of a Rapid and Convenient Fluoroimmunoassay Platform Using Antibodies Against PDL1 and HER2 DOI Creative Commons
Ji Eun Choi,

Hanool Yun,

Hee‐Jin Jeong

et al.

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(1), P. 62 - 62

Published: Jan. 17, 2025

The development of accurate and high-throughput tools for cancer biomarker detection is crucial the diagnosis, monitoring, treatment diseases. In this study, we developed a simple rapid fluorescence-linked immunosorbent assay (FLISA) using fluorescent dye-conjugated antibody fragments against programmed cell death ligand 1 (PDL1) human epithelial growth factor receptor 2 (HER2). We optimized key steps in FLISA process, including antigen immobilization, blocking, reaction, reading time to 3 h-significantly faster compared 23 h duration usual FLISA. limit detecting PDL1 was lower than that FLISA, HER2 similar between two methods, indicating provides fast approach HER2. This robust platform can be readily adapted various fluoroimmunoassays targeting other antigens interest.

Language: Английский

Citations

0
A Time-Resolved Fluorescent Microsphere Immunochromatographic Assay for Determination of Vitamin B12 in Infant Formula Milk Powder DOI Creative Commons
Qianqian Lü,

Yongwei Feng,

Qi Zhou

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(2), P. 65 - 65

Published: Jan. 21, 2025

Vitamin B12 (VB12) is an important nutrient, and its quality control in food crucial. In this study, based on the principle of specific recognition target analyte by monoclonal antibodies (mAbs), a time-resolved fluorescent microsphere immunochromatographic assay (TRFM-ICA) was developed to detect content VB12 infant formula milk powder. First, performance anti-VB12 mAb evaluated, revealing half-maximal inhibitory concentration 0.370 ng/mL, affinity constant 2.604 × 109 L/mol no cross-reactivity with other vitamins. Then, highly sensitive TRFM-ICA developed, visual limit detection 10 μg/kg cut-off value 100 for qualitative range 4.125–82.397 quantitative detection. addition, test results real samples were consistent quantification using microbiological methods, coefficient variation less than 10%, showing good accuracy stability, confirming that suitable analysis powder samples. (mAbs) against VB12, fluorescence converting biological signals into optical signals.

Language: Английский

Citations

0
Advanced sensing strategies for detecting zinc levels and zinc-related biomarkers in cancer pathogenesis DOI Creative Commons
Daniela Vieira,

Graziele Cruzado,

Edward J. Harvey

et al.

Sensing and Bio-Sensing Research, Journal Year: 2025, Volume and Issue: 47, P. 100754 - 100754

Published: Jan. 31, 2025

Language: Английский

Citations

0
An ultrasensitive electrochemical sensor for protein biomarker detection based on silver nanoflowers labeling DOI
Qixing Liu, Bin Wu, Zi‐Han Chen

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113338 - 113338

Published: March 1, 2025

Language: Английский

Citations

0
Nanolabels Prepared by the Entrapment or Self-Assembly of Signaling Molecules for Colorimetric and Fluorescent Immunoassays DOI Creative Commons
Ning Xia,

Y.-C. Li,

Cancan He

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(12), P. 597 - 597

Published: Dec. 6, 2024

Nanomaterials have attracted significant attention as signal reporters for immunoassays. They can directly generate detectable signals or release a large number of signaling elements readout. Among various nanolabels, nanomaterials composed multiple molecules shown great potential in Generally, be entrapped nanocontainers self-assemble into nanostructures amplification. In this review, we summarize the advances molecules-entrapped assembled colorimetric and fluorescence The cover liposomes, polymers, mesoporous silica, metal–organic frameworks (MOFs), nanosheets, nanoflowers nanocages, etc. Signaling mainly refer to visible and/or fluorescent organic dyes. design application immunoassays are emphasized from perspective nanocontainers, analytes, analytical performances. addition, future challenges research trends preparation nanolabels briefly discussed.

Language: Английский

Citations

1