Taxonomic Identification, Complete Genome Sequencing, and In Silico Genome Mining of the Actinobacterium Lentzea sp. JNUCC 0626 Isolated from Jeju Gotjawal DOI Open Access
Kyung‐A Hyun, Kyung-Hwan Boo, Chang‐Gu Hyun

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: 70(1), P. 8 - 8

Published: Feb. 7, 2025

In our previous study, Lentzea sp. JNUCC 0626 was isolated from Hwasun Gotjawal on Jeju Island, and its melanogenic effects were confirmed in B16F10 melanoma cells through the identification of 1-acetyl-β-carboline. this we conducted a comprehensive taxonomic characterization 0626, including enzymatic activities, carbohydrate metabolism, growth conditions, cellular composition. Major fatty acids identified iso-C16:0, iso-C15:0, C15:0 anteiso, with polar lipids such as diphosphatidylglycerol, phosphatidylethanolamine, several unidentified lipids. Ubiquinone Q-9 determined predominant respiratory quinone. Enzymatic activity analysis (API ZYM) showed alkaline phosphatase, esterase (C4), lipase (C8), leucine arylamidase while metabolism 50CHB) indicated acid production esculin alone. Complete genome sequencing revealed 10,602,950 bp linear chromosome 177,940 plasmid. This plasmid encodes essential plasmid-related genes, Type IV secretion system ParA proteins critical for transfer stability. These findings suggest that could be utilized developing host–vector systems to facilitate combinatorial biosynthesis novel bioactive compounds. Comparative genomic pudingi CGMCC 4.7319 closest relative, but significant genetic divergence (dDDH 46.7%, ANI 88.02%) strongly supports classification species. AntiSMASH 34 biosynthetic gene clusters (BGCs), highlighting strain’s capacity produce diverse Finally, extract exhibited concentration-dependent NO inhibition LPS-stimulated RAW 264.7 cells, demonstrating anti-inflammatory activity. suggests secondary metabolites inferred may contribute these observed bioactivities.

Language: Английский

Taxonomic Identification, Complete Genome Sequencing, and In Silico Genome Mining of the Actinobacterium Lentzea sp. JNUCC 0626 Isolated from Jeju Gotjawal DOI Open Access
Kyung‐A Hyun, Kyung-Hwan Boo, Chang‐Gu Hyun

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: 70(1), P. 8 - 8

Published: Feb. 7, 2025

In our previous study, Lentzea sp. JNUCC 0626 was isolated from Hwasun Gotjawal on Jeju Island, and its melanogenic effects were confirmed in B16F10 melanoma cells through the identification of 1-acetyl-β-carboline. this we conducted a comprehensive taxonomic characterization 0626, including enzymatic activities, carbohydrate metabolism, growth conditions, cellular composition. Major fatty acids identified iso-C16:0, iso-C15:0, C15:0 anteiso, with polar lipids such as diphosphatidylglycerol, phosphatidylethanolamine, several unidentified lipids. Ubiquinone Q-9 determined predominant respiratory quinone. Enzymatic activity analysis (API ZYM) showed alkaline phosphatase, esterase (C4), lipase (C8), leucine arylamidase while metabolism 50CHB) indicated acid production esculin alone. Complete genome sequencing revealed 10,602,950 bp linear chromosome 177,940 plasmid. This plasmid encodes essential plasmid-related genes, Type IV secretion system ParA proteins critical for transfer stability. These findings suggest that could be utilized developing host–vector systems to facilitate combinatorial biosynthesis novel bioactive compounds. Comparative genomic pudingi CGMCC 4.7319 closest relative, but significant genetic divergence (dDDH 46.7%, ANI 88.02%) strongly supports classification species. AntiSMASH 34 biosynthetic gene clusters (BGCs), highlighting strain’s capacity produce diverse Finally, extract exhibited concentration-dependent NO inhibition LPS-stimulated RAW 264.7 cells, demonstrating anti-inflammatory activity. suggests secondary metabolites inferred may contribute these observed bioactivities.

Language: Английский

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