Phytochemical analysis of Stachys iva: Discovering the optimal extract conditions and its bioactive compounds

Open Life Sciences, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 1, 2025

The Lamiaceae family is one of the widest plant families among Greek flora, consisting a great variety species, with genus Stachys being its largest representatives, spread to most continents. also known for beneficial properties and has been used years as traditional remedy healing various health conditions. iva, an endemic in Kozani Regional unit, consumed infusion by locals reported relieve common cold symptoms, have antimicrobial properties, contribute normalizing blood glucose levels. present study aimed identify chemical compounds (such phenolic acids, flavonoids, phenylethanoid glycosides) responsible herb's pharmacological determine optimal extraction conditions gather extract high therapeutic value without solvent energy waste. Experiments conducted proved that extracting simple stirring deionized water 75 min at 80°C best option. In contrast, extract's total polyphenol content was determined, were identified high-performance liquid chromatography analysis. addition, other methods utilized (e.g., ferric-reducing antioxidant power assay 2,2-diphenyl-1-picrylhydrazyl antiradical activity assay) reveal potent antioxidant, anti-hydrogen peroxide, anti-inflammatory activity, while correlation between these examined.

Language: Английский

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Ultrasound-assisted extraction of Stachys chasmosericea : phytochemical profile, antioxidant potentials via RACI analysis, and enzyme inhibitory activities

Preparative Biochemistry & Biotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 10

Published: April 1, 2025

Understanding the bioactive potential of plant extracts requires detailed evaluations their chemical composition and bioactivities. This study investigated profiles, antioxidant properties, enzyme inhibitory activities water methanol Stachys chasmosericea Ayasligil & P.H.Davis obtained via ultrasound-assisted extraction. The extract displayed significantly higher total phenolic flavonoid contents compared to extract. Chromatographic analysis revealed verbascoside chlorogenic acid as dominant compounds in extract, whereas contained levels polar like p-coumaric acid. Antioxidant assays demonstrated extract's superior activity. Conversely, excelled metal chelation. RACI values confirmed overall superiority (RACI = 0.65) over −0.65). Enzyme inhibition stronger activity against AChE, BChE, α-amylase, α-glucosidase, tyrosinase showed weaker inhibition. Both for managing oxidative stress enzyme-related disorders but require further vivo validation. highlights S. chasmosericea, particularly its a promising source natural antioxidants inhibitors. Future research should focus on isolating individual bioactives exploring mechanisms action biological systems.

Language: Английский

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Polyphenolic Composition, Mineral Profile, and Biological Activities in Different Organs of Alpine Woundwort—Insights into Antioxidant and Enzyme Inhibitory Potential

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(5), P. 674 - 674

Published: May 1, 2025

Background: Stachys alpina is a medicinal plant from the Lamiaceae family whose biological potential remains poorly explored. Methods: The aim of this study was to comprehensively assess pol-yphenol profile, macro- and microelement composition, antioxidant, an-ti-diabetic, anti-obesity activities various organs (leaves, flowers, stems, roots). Results: leaves flowers exhibited highest concentration phenolic compounds, while anthocyanins were detected exclusively in (215.05 mg/100 g dry matter (dm)) constituted 3% total polyphenols. Verbas-coside chlorogenic acid most abundant polyphenols, reaching 4618.88 3277.83 mg/ 100 dm leaves. ABTS FRAP scavenging activity observed (19.30 7.62 mmol TE/g dm, respectively). Principal component analysis demonstrated strong correlation between polyphenol content antioxidant (ABTS-r= 0.87 FRAP-r = 0.90), which further confirmed by Pearson’s coefficients. also highlighted significant impact mineral composition on activity—calcium magnesium dominated stems (10,100 3900 mg/kg) roots (9200 3100 mg/kg), supporting functioning an-tioxidant enzymes, zinc manganese (89.43 155.33 con-tributed intense metabolic processes. Conclusions: S. aplina could serve as valuable source natural antioxidants enzyme inhibitors associated with glucose lipid metabolism, suggesting its promising application prevention management disorders..

Language: Английский

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A contribution to the knowledge of wild medicinal Lamiaceae: Taxonomic, microscopic, and ecological insights from palynology

Genetic Resources and Crop Evolution, Journal Year: 2025, Volume and Issue: unknown

Published: May 9, 2025

Language: Английский

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