Synergistic effects of Pandanus fascicularis extracts and azithromycin: in vitro and in silico antimicrobial investigation against MDR clinical strains DOI Creative Commons
Hajera Khatun, Md Rashedul Islam,

Shikha Khatun

et al.

Frontiers in Antibiotics, Journal Year: 2025, Volume and Issue: 4

Published: May 29, 2025

Background Antibiotic-resistant bacteria are becoming a significant global concern. To combat the spread of resistance or reverse multidrug resistance, developing novel antimicrobials and/or modulators is essential. This study aimed to evaluate synergistic effects methanolic extract Pandanus fascicularis fruits (MEPFF) in combination with azithromycin against multidrug-resistant bacteria. Methods Phytochemical analysis along determination total phenolic content (TPC), flavonoid (TFC), and antioxidant capacity (TAC) MEPFF, was performed using standard procedure. The extract's DPPH free radical scavenging activity assessed its potential activity. minimal inhibitory concentration (MIC) bactericidal (MBC) MEPFF Staphylococcus aureus , Bacillus cereus Escherichia coli Pseudomonas aeruginosa were determined, followed by an effect azithromycin, as well alone. Subsequently, drug-likeness, antibacterial activity, toxicological properties analyzed silico tools. Results quantitative investigation found terpenoids, flavonoids, tannins, phenolics, saponins, cardiac glycosides, alkaloids MEPFF. TFC, TPC, TAC at 183 ± 9.54 mg QE, 248.33 11.06 GAE, 95.33 8.33 AAE/gm extract. showed experiment, IC50 value 12.13±0.53 µg/ml. Azithromycin together have far greater action all four bacterial strains. 3.67 1.15 5.83 0.76 mg/mL, while minimum 4.33 1.26 7.33 1.04 mg/mL. In studies revealed that pandamarilactone-1, nonpandamarilactone-B, thiamine had best docking energy (−9.9, −8.9, −8.5 kcal/mol), suggesting most active compounds MPh-II protein. Conclusion enhances antibiotic therapy suggests aforesaid drug-herb combinations may treat MDR infections.

Language: Английский

Impact of a Dietary Supplementation with French Maritime Pine Bark Extract Pycnogenol® on Salivary and Serum Inflammatory Biomarkers During Non-Surgical Periodontal Therapy—A Randomized Placebo-Controlled Double-Blind Trial DOI Open Access

Jasmin Bayer,

Nicole Petersen,

Jeanine Veruschka Hess

et al.

Nutrients, Journal Year: 2025, Volume and Issue: 17(9), P. 1546 - 1546

Published: April 30, 2025

Background: Gingival inflammation is highly prevalent and may impact systemic health. While professional mechanical plaque removal (PMPR) the standard treatment, dietary interventions provide additional benefits. The French maritime pine bark extract Pycnogenol® has anti-inflammatory antioxidant properties, but its on inflammatory biomarkers in saliva serum not been studied a controlled clinical trial. Methods: In this randomized, double-blind, placebo-controlled trial, 91 participants received (100 mg twice daily; n = 46) or placebo (n 45) following PMPR. Saliva samples were collected at baseline, after two three months. Inflammatory (IL-1β, IL-6, MMP-8, MMP-9) polyphenol concentrations analyzed using ELISA LC-MS/MS. Results: supplementation significantly reduced salivary MMP-8 levels (p 0.0261), IL-6 compared to 0.0409). Additionally, ferulic acid, caffeic gut microbial metabolite 5-(3,4-dihydroxyphenyl)-γ-valerolactone (M1) increased intake. A correlation analysis revealed significant inverse association between bleeding probing M1 concentration (r -0.3476, p 0.0167). Conclusions: Dietary with key saliva, suggesting potential effect of gingival inflammation. Trial registration: ClinicalTrials.gov (NCT05786820).

Language: Английский

Citations

0
Advances in harnessing biological macromolecules for periodontal tissue regeneration: A review DOI

Dongyi Yang,

Dong He, Fengkai Yang

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 144031 - 144031

Published: May 1, 2025

Language: Английский

Citations

0
Synergistic effects of Pandanus fascicularis extracts and azithromycin: in vitro and in silico antimicrobial investigation against MDR clinical strains DOI Creative Commons
Hajera Khatun, Md Rashedul Islam,

Shikha Khatun

et al.

Frontiers in Antibiotics, Journal Year: 2025, Volume and Issue: 4

Published: May 29, 2025

Background Antibiotic-resistant bacteria are becoming a significant global concern. To combat the spread of resistance or reverse multidrug resistance, developing novel antimicrobials and/or modulators is essential. This study aimed to evaluate synergistic effects methanolic extract Pandanus fascicularis fruits (MEPFF) in combination with azithromycin against multidrug-resistant bacteria. Methods Phytochemical analysis along determination total phenolic content (TPC), flavonoid (TFC), and antioxidant capacity (TAC) MEPFF, was performed using standard procedure. The extract's DPPH free radical scavenging activity assessed its potential activity. minimal inhibitory concentration (MIC) bactericidal (MBC) MEPFF Staphylococcus aureus , Bacillus cereus Escherichia coli Pseudomonas aeruginosa were determined, followed by an effect azithromycin, as well alone. Subsequently, drug-likeness, antibacterial activity, toxicological properties analyzed silico tools. Results quantitative investigation found terpenoids, flavonoids, tannins, phenolics, saponins, cardiac glycosides, alkaloids MEPFF. TFC, TPC, TAC at 183 ± 9.54 mg QE, 248.33 11.06 GAE, 95.33 8.33 AAE/gm extract. showed experiment, IC50 value 12.13±0.53 µg/ml. Azithromycin together have far greater action all four bacterial strains. 3.67 1.15 5.83 0.76 mg/mL, while minimum 4.33 1.26 7.33 1.04 mg/mL. In studies revealed that pandamarilactone-1, nonpandamarilactone-B, thiamine had best docking energy (−9.9, −8.9, −8.5 kcal/mol), suggesting most active compounds MPh-II protein. Conclusion enhances antibiotic therapy suggests aforesaid drug-herb combinations may treat MDR infections.

Language: Английский

Citations

0