Shifts in sulphur-cycling bacteria in the rhizobiome support the adaptation of Caulerpa prolifera to elevated sulphide levels DOI Creative Commons

Anastasiia Barilo,

Aschwin H. Engelen, Susanne Wilken

et al.

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 11

Published: Oct. 24, 2024

Caulerpa is a genus of green macroalgae that lives in tropical and subtropical coastal waters. It an intriguing organism because, despite having plant-like structures, it one giant cell – which, next to multiple nuclei, chloroplasts, mitochondria, also contains endo- epiphytic bacteria. The role these bacteria unknown, but they might impact the growth development host, adaptation environmental parameters, and, hence, ecological success algae. We hypothesised increased sulphide concentrations would trigger significant shift microbial community composition associated with C. prolifera rhizoids, favouring sulphide-oxidizing To test this hypothesis, we conducted mesocosm experiment incubating sediments different analysed algal photosynthesis, growth, microbiome composition. While photosynthesis was not affected, weight-based rate decreased linearly increasing concentration. analyse microbiome, extracted DNA RNA from fronds, accompanying performed 16S amplicon sequencing. fronds unaffected both samples. However, increase concentration coincided decrease relative abundance sulphate-reducing particularly family Desulfocapsaceae. In samples, potential oxidisers rhizoid-associated members Beggiatoaceae were detected. Our results suggest rhizobiome plays its sulphide-rich environments, offering new insights into complex interactions within marine holobionts.

Language: Английский

Comparison of the performance of MiSeq and NovaSeq in oral microbiome study DOI Creative Commons
Hyejung Han, Hyo‐Jung Lee, Keun‐Suh Kim

et al.

Journal of Oral Microbiology, Journal Year: 2024, Volume and Issue: 16(1)

Published: April 18, 2024

Objective Next generation sequencing is commonly used to characterize the microbiome structure. MiSeq most analyze due its relatively long read length. Illumina also introduced 250 × 2 chip for NovaSeq. The purpose of this study was compare performance and NovaSeq in context oral study.

Language: Английский

Citations

1
Distinctive salivary oral microbiome in patients with burning mouth syndrome depending on pain intensity compared to healthy subjects DOI Creative Commons

Hye‐Min Ju,

Yong‐Woo Ahn, Soo‐Min Ok

et al.

Journal of Dental Sciences, Journal Year: 2024, Volume and Issue: 20(1), P. 462 - 469

Published: May 24, 2024

Burning moouth syndrome (BMS) is a chronic pain condition similar to neuropathic pain. It characterized by persistent burning sensation in the oral cavity. Despite lack of clarity regarding etiology BMS, recent studies have reported an association between gut microbiome and However, few investigated orofacial pain, such as BMS. This study aimed compare microbial profiles healthy controls (HC) patients with The BMS group was further divided into BMS_low BMS_high groups according intensity. A total 60 (BMS_low, n = 16; BMS_high, 44) 30 HC provided saliva samples, which were sequenced analyzed for V1-V2 region 16S rRNA gene. alpha diversity among three groups. significant difference distribution composition observed HC, revealed Bray-Curtis distance analysis (P < 0.01). At genus level, Prevotella Alloprevotella most abundant genera group. Compared exhibited relatively higher abundance bacterial species. Some bacteria, including spp., exhibit increasing pattern subjective These results suggest potential involvement microbiota pathogenesis. Additionally, variations may occur not only presence or absence but also severity.

Language: Английский

Citations

1
Impact of cell lysis treatment before saliva metagenomic DNA extraction on the oral microbiome and the associated resistome DOI Creative Commons
Supathep Tansirichaiya, Kittikun Songsomboon, Nichamon Chaianant

et al.

Clinical and Experimental Dental Research, Journal Year: 2024, Volume and Issue: 10(4)

Published: June 28, 2024

The human oral microbiome, a complex ecosystem linked to and systemic health, harbors diverse array of microbial populations, including antimicrobial resistance genes (ARGs). As critical component the One Health approach tackle antibiotic resistance, comprehending resistome's composition diversity is imperative. objective this study was investigate impact chemical cell lysis treatment using MetaPolyzyme on detectability resistome, DNA quality quantity.

Language: Английский

Citations

1
Exploring the diversity and functional profile of microbial communities of Brazilian soils with high salinity and oil contamination DOI Creative Commons
Danielly C. O. Mariano, Graciela Maria Dias,

Michele R. Castro

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(14), P. e34336 - e34336

Published: July 1, 2024

Environmental pollution associated with the petroleum industry is a major problem worldwide. Microbial degradation extremely important whether in extractive process or bioremediation of contaminants. Assessing local microbiota and its potential for crucial implementing effective strategies. Herein, contaminated soil samples onshore oil fields from semiarid region Northeast Brazil were investigated using metagenomics metataxonomics. These soils exhibited hydrocarbon contamination high salinity indices, while control sample was collected an uncontaminated area. The shotgun analysis revealed predominance Actinomycetota Pseudomonadota, 16S rRNA gene amplicon showed Actinomycetota, Bacillota, Pseudomonadota as most abundant. Archaea domain phylotypes assigned to Thermoproteota Methanobacteriota. Functional metabolic profile microbiomes broader repertoire soil, pathways surfactant biosynthesis presented higher values soils, where xenobiotic aromatic compounds also present. Biosurfactant synthetic abundant, lipopeptides. present work uncovers several microbial drivers mechanisms adaptation salinity, which are pivotal traits sustainable recovery

Language: Английский

Citations

1
Microbiomic insights into the oral microbiome’s role in type 2 diabetes mellitus: standardizing approaches for future advancements DOI Creative Commons
Huifang Guan, Shuang Zhao,

Yuanfei Tan

et al.

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 29, 2024

The burgeoning field of microbiomics has unveiled significant insights into the role oral microbiome in pathophysiology Type 2 Diabetes Mellitus (T2DM), with this review focusing on recent advancements diabetic microbiology, its clinical applications, and identifying factors that may affect study interpretations. A comprehensive across various databases, including PubMed Google Scholar, was conducted to collate original research data published past five years, specifically targeting studies exploring impact T2DM emphasizing employs microbiomic approaches patient populations. findings delineate intricate interplay between dysbiosis, highlighting microbial shifts following periodontal antidiabetic treatments, pointing complexity relationship health systemic disease. observed underscore critical need for standardized methodologies studies, suggesting by adopting a unified approach, future can more effectively elucidate microbiome's T2DM. This could pave way innovative diagnostic therapeutic strategies managing complications, thus making pertinent overview work within field.

Language: Английский

Citations

1
Microbial Profiles in Oral Lichen Planus: Comparisons with Healthy Controls and Erosive vs. Non-Erosive Subtypes DOI Creative Commons

Hye‐Min Ju,

Yong‐Woo Ahn, Soo‐Min Ok

et al.

Diagnostics, Journal Year: 2024, Volume and Issue: 14(8), P. 828 - 828

Published: April 17, 2024

Recent studies have begun exploring the potential involvement of microbiota in pathogenesis oral lichen planus (OLP), yet comprehensive investigations remain limited. Hence, this study aimed to compare microbial profiles saliva samples obtained from patients with OLP against those healthy controls (HC), along a comparison between erosive (E) and non-erosive (NE) patients. Saliva were collected 60 (E: n = 25, NE: 35) 30 HC individuals. Analysis revealed no significant differences alpha diversity, as assessed by Chao1 Shannon index, across three groups. However, Bray–Curtis distance analysis indicated disparity microbiome composition distribution E-OLP, well NE-OLP The six most abundant phyla observed groups Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, Fusobacteria, Saccharibacteria (TM7). Notably, exhibited higher prevalence Bacteroidetes. Prevotella emerged predominant genus groups, while Capnocytophaga showed relatively E-OLP compared NE-OLP. This study’s findings indicate notable difference OLP. Additionally, identified increase proportion certain bacterial species suggests that they may exacerbate inflammatory response act antigens for

Language: Английский

Citations

0
Soil pH, developmental stages and geographical origin differently influence the root metabolomic diversity and root-related microbial diversity of Echium vulgare from native habitats DOI Creative Commons
Cintia Csorba, Nebojša Rodić, Livio Antonielli

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: June 24, 2024

Improved understanding of the complex interaction between plant metabolism, environmental conditions and plant-associated microbiome requires an interdisciplinary approach: Our hypothesis in our multiomics study posited that several biotic factors have modulating effects on metabolome roots wild Echium vulgare plants. Furthermore, we postulated reciprocal interactions root microbiome. We investigated metabolic content, genetic variability, prokaryotic systems E. plants at rosette flowering stages across six distinct locations. incorporated assessment soil microbiomes measurement selected chemical composition factors. Two clusters were determined based microsatellite analysis without a consistent alignment with geographical proximity The microbial diversity both surrounding bulk exhibited significant divergence locations, varying pH characteristics, within identified clusters. Notably, acidophilic bacteria characteristic inhabitants under acidic conditions, emphasizing close interconnectedness these compartments. significantly differed samples from different developmental stages, levels. stage was dominant driver changes, higher concentrations sugars, pyrrolizidine alkaloids, some their precursors roots. featured dynamics pH, development, genetics, microbiome, shedding light existing knowledge gaps.

Language: Английский

Citations

0
Oral specimens as a tool for accurate metagenomic analysis: A pilot study DOI Creative Commons
Manuel Eros Rodríguez-Fuentes, Mariana de Sá Alves, Gema Barbeito‐Castiñeiras

et al.

Journal of Stomatology Oral and Maxillofacial Surgery, Journal Year: 2024, Volume and Issue: 125(5), P. 101991 - 101991

Published: July 30, 2024

Acute oral mucosal damage, as well other inflammatory processes seem to be related dysbiosis of the microbiome. The need study changes in microbiome led us hypothesize what type sample would provide most representative picture entire human An observational, and cross-sectional was carried out. Six healthy adult participants provided 3 different types each, that included saliva, rinse biopsy tissue. We performed 16S rRNA sequencing V3-V4 region 18 samples using Illumina MiSeq technology. Participants were 27 ± 6,3 years old. Bacterial alpha diversity higher compared whole unstimulated saliva mucosa tissue (p = 0,005). However, specimens showed a 56 % relative abundance identified species followed by 30 only 1 samples. This found differences on composition for each sample. Oral should chosen when is needed, whereas more appropriate larger amount bacterial DNA. results obtained demonstrate importance correct choice optimal studies due its composition.

Language: Английский

Citations

0
Clinical Evaluation of Microbial Communities and Associated Biofilms with Breast Augmentation Failure DOI Creative Commons

Robert J. Whitfield,

Craig D. Tipton,

Niccole Diaz

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(9), P. 1830 - 1830

Published: Sept. 4, 2024

The incidence of breast implant illness (BII) and BII-related explant procedures has not decreased with current surgical treatment techniques. It is speculated the main underlying cause BII complications result chronic, sub-clinical infections residing on around implant. infection, subsequent biofilm, produce antagonistic compounds that drive chronic inflammation immune responses. In this study, microbial communities in over 600 consecutive samples infected capsules tissues were identified via next-generation sequencing to identify any commonality between samples. majority bacteria Gram-positive,

Language: Английский

Citations

0
Shifts in sulphur-cycling bacteria in the rhizobiome support the adaptation of Caulerpa prolifera to elevated sulphide levels DOI Creative Commons

Anastasiia Barilo,

Aschwin H. Engelen, Susanne Wilken

et al.

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 11

Published: Oct. 24, 2024

Caulerpa is a genus of green macroalgae that lives in tropical and subtropical coastal waters. It an intriguing organism because, despite having plant-like structures, it one giant cell – which, next to multiple nuclei, chloroplasts, mitochondria, also contains endo- epiphytic bacteria. The role these bacteria unknown, but they might impact the growth development host, adaptation environmental parameters, and, hence, ecological success algae. We hypothesised increased sulphide concentrations would trigger significant shift microbial community composition associated with C. prolifera rhizoids, favouring sulphide-oxidizing To test this hypothesis, we conducted mesocosm experiment incubating sediments different analysed algal photosynthesis, growth, microbiome composition. While photosynthesis was not affected, weight-based rate decreased linearly increasing concentration. analyse microbiome, extracted DNA RNA from fronds, accompanying performed 16S amplicon sequencing. fronds unaffected both samples. However, increase concentration coincided decrease relative abundance sulphate-reducing particularly family Desulfocapsaceae. In samples, potential oxidisers rhizoid-associated members Beggiatoaceae were detected. Our results suggest rhizobiome plays its sulphide-rich environments, offering new insights into complex interactions within marine holobionts.

Language: Английский

Citations

0