polyethylene
glycol
(PEG),
to
evade
the
immune
system;
ii)
NCs
modified
with
HA,
CD44-HA
binding
and
iii)
non-modified
(carboxyl
moiety).Macrophages
previously
exposed
were
co-cultured
AML
cells
uptake
delivery
of
analyzed
by
flow
cytometry.As
a
consequence
CD44
increased
intensity,
(PLGA)-PEG-HA
adhered
membrane
pro-leukemic
macrophages.Also,
delivered
blasts
cell-to-cell
interaction,
accumulating
into
leukemic
cell
increasing
cancer
cells'
death.Overall,
our
results
suggest
that
macrophage-based
deliver
(PLGA)PEG-HA
loaded
ATO
is
promising
platform
treat
as
can
improve
targetability
adhering
AML-related
macrophages
reduce
viability
in
vitro.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2021,
Volume and Issue:
unknown
Published: Dec. 10, 2021
ABSTRACT
Owing
to
their
diagnostic
and
therapeutic
potential,
extracellular
vesicles
(EVs)
derived
from
tumour
cells
have
recently
garnered
great
interest.
The
presence
of
different
glycosylation
sites
at
the
EV
surface
supports
need
for
efficient
glycosylated
isolation.
Here,
we
developed
a
GlyExo-Capture
technique
robustly
capturing
fucosylated
EVs
sera
cell
supernatants.
Lens
culinaris
lectin
(LCA)-immobilized
magnetic
complexes
were
found
capture
approximately
60%
total
HepG2
cells.
efficiency
was
reduced
less
than
40%
in
nontumorigenic
MIHA
Notably,
cellular
uptake
pattern
highly
markedly
that
with
low
fucosylation.
unearthing
enriched
miRNA
cargos
by
next-generation
deep
sequencing
(NGS)
revealed
75
differentially
expressed
miRNAs
(DEMs)
hepatocellular
carcinoma
(HCC).
Among
them,
4-miRNA
panel
chosen
yielded
an
area
under
ROC
curve
(AUC)
0.86
0.84
detection
HCC
non-HCC
controls
testing
samples
independent
validation
samples,
respectively.
signature
alpha-fetoprotein
(AFP),
combined
model
AFP
increased
AUC
0.92.
In
conclusion,
high-throughput
method
efficiently
shed
light
on
use
as
potential
sources
cancer
biomarker
detection.
Pharmacognosy Magazine,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 24, 2024
Background
Non-small-cell
lung
cancer
is
the
most
ubiquitous
malignancy,
and
present
treatments
do
not
offer
effective
outcomes
for
therapy.
Natural
drug
therapy,
which
directly
targets
tumor
cells,
now
recognized
as
a
safe
technique
treatment.
The
use
of
tailored
nanoparticles
also
improves
efficacy
Purpose
To
synthesize,
characterize,
evaluate
natural
concanavalin
A
(ConA)
coated
with
chitosan
(ConA-CS
NPs)
cytotoxicity
against
cell
line
(NCI-H358).
Methods
current
study
demonstrates
development
ConA-CS
NPs
well
their
unique
characteristics,
anticancer
processes,
possible
applications
in
Using
UV–Vis,
Fourier
transform
infrared,
X-ray
diffraction,
field
emission
scanning
electron
microscopy,
dynamic
light
scattering
studies,
were
generated
evaluated.
mitochondrial
toxicity
test
assay
was
used
to
examine
NP-induced
cytotoxicity.
Dual
(acridine
orange/ethidium
bromide)
staining
performed
apoptotic
modifications,
membrane
potential
levels
NCI-H358
cells
examined
using
appropriate
fluorescence
assays.
Results
Conclusion
enhanced
treatment,
combines
drugs
eradicate
prevent
relapses,
supported
by
vitro
investigations.
ability
ConA-encapsulated
CS
impede
human
carcinoma
growth
has
yet
been
investigated.
Therefore,
this
investigation
may
provide
new
paradigm
detection
results
surely
help
enhance
quality
life
patients.
polyethylene
glycol
(PEG),
to
evade
the
immune
system;
ii)
NCs
modified
with
HA,
CD44-HA
binding
and
iii)
non-modified
(carboxyl
moiety).Macrophages
previously
exposed
were
co-cultured
AML
cells
uptake
delivery
of
analyzed
by
flow
cytometry.As
a
consequence
CD44
increased
intensity,
(PLGA)-PEG-HA
adhered
membrane
pro-leukemic
macrophages.Also,
delivered
blasts
cell-to-cell
interaction,
accumulating
into
leukemic
cell
increasing
cancer
cells'
death.Overall,
our
results
suggest
that
macrophage-based
deliver
(PLGA)PEG-HA
loaded
ATO
is
promising
platform
treat
as
can
improve
targetability
adhering
AML-related
macrophages
reduce
viability
in
vitro.
