Journal of Functional Biomaterials,
Journal Year:
2024,
Volume and Issue:
15(12), P. 369 - 369
Published: Dec. 6, 2024
This
study
aimed
to
investigate
the
impact
of
varying
formulation
a
specific
peptide
hydrogel
(PepGel)
on
release
kinetics
rhBMP-2
in
vitro.
Three
PepGel
formulations
were
assessed:
(1)
50%
v/v
(peptides
volume/total
volume)
PepGel,
where
synthetic
peptides
mixed
with
crosslinking
reagents
and
solution;
(2)
67%
PepGel;
(3)
80%
PepGel.
Each
sample
was
loaded
12
µg
incubated
PBS.
Released
quantified
by
ELISA
at
1
h,
6
1,
2,
4,
7,
10,
14,
21
days.
To
explore
how
influence
release,
gel
porosities,
swelling
ratios,
mechanical
properties
three
quantitatively
analyzed.
The
results
showed
that
encapsulated
exhibited
sustained
over
21-day
experiment,
while
PepGels
demonstrated
significantly
lower
rates
compared
after
day
7.
Higher
histological
porosity
correlated
increased
rates.
Conversely,
ratio
elastic
modulus
than
formulations.
In
conclusion,
this
indicates
crosslinked
can
control
vitro
modulating
porosity,
ratio,
properties.
Encapsulation
offers
pattern
vitro;
if
replicated
vivo,
could
mitigate
adverse
effects
associated
burst
clinical
applications.
Biomedicines,
Journal Year:
2022,
Volume and Issue:
10(10), P. 2342 - 2342
Published: Sept. 20, 2022
Cyclodipeptides
(CDPs)
or
diketopiperazines
(DKPs)
are
often
found
in
nature
and
foodstuff
beverages
have
attracted
great
interest
for
their
bioactivities,
biocompatibility,
biodegradability.
In
the
laboratory,
they
can
be
prepared
by
green
procedures,
such
as
microwave-assisted
cyclization
of
linear
dipeptides
water,
performed
this
study.
particular,
five
CDPs
were
characterized
a
variety
methods,
including
NMR
ESI-MS
spectroscopies
single-crystal
X-ray
diffraction
(XRD),
cytocompatibility
anti-aging
activity
was
tested
vitro,
well
ability
to
penetrate
different
layers
skin.
Although
mechanism
action
remains
elucidated,
proof-of-concept
study
lays
basis
future
use
anti-age
cosmetic
applications.
Polymers,
Journal Year:
2022,
Volume and Issue:
14(21), P. 4554 - 4554
Published: Oct. 27, 2022
There
is
an
increasing
interest
towards
the
development
of
new
antimicrobial
coatings,
especially
in
light
emergence
resistance
(AMR)
common
antibiotics.
Cyclodipeptides
(CDPs)
or
diketopiperazines
(DKPs)
are
attractive
candidates
for
their
ability
to
self-assemble
into
supramolecular
polymers
and
yield
gel
coatings
that
do
not
persist
environment.
In
this
work,
we
compare
cyclo(Leu-Phe)
with
its
heterochiral
analogs
cyclo(D-Leu-L-Phe)
cyclo(L-Leu-D-Phe),
as
well
cyclo(L-Phe-D-Phe),
gel.
The
compounds
were
synthesized,
purified
by
HPLC,
characterized
The
divergent
supramolecular
behavior
of
a
series
tripeptide
stereoisomers
was
elucidated
through
spectroscopic,
microscopic,
crystallographic
and
computational
techniques.
Only
two
epimers
were
able
to
effectively
self-organize
into
amphipathic
structures,
leading
hydrogels
or
crystals,
respectively.
Despite
the
similarity
between
peptides’
turn
conformations,
stereoconfiguration
led
different
abilities
engage
in
intramolecular
hydrogen
bonding.
Self-assembly
further
shifted
pKa
C-terminal
side
chain.
As
result,
across
pH
range
4-6,
only
one
epimer
predominated
sufficiently
as
zwitterion
reach
critical
molar
fraction
allowing
gelation.
By
contrast,
differing
values
higher
dipole
moment
other
favored
crystallization.
four
tested
for
gold
nanoparticle
(Au
NP)
formation,
with
hydrogel
being
key
control
stabilize
Au
NPs,
yielding
nanocomposite
that
catalyzed
photodegradation
dye.
Importantly,
NP
formation
occurred
without
use
reductants
than
peptide,
redox
chemistry
investigated
by
LC-MS
NMR.
This
study
provides
important
insights
rational
design
simple
peptides
minimalistic
green
building
blocks
functional
nanocomposites.
Journal of Functional Biomaterials,
Journal Year:
2024,
Volume and Issue:
15(12), P. 369 - 369
Published: Dec. 6, 2024
This
study
aimed
to
investigate
the
impact
of
varying
formulation
a
specific
peptide
hydrogel
(PepGel)
on
release
kinetics
rhBMP-2
in
vitro.
Three
PepGel
formulations
were
assessed:
(1)
50%
v/v
(peptides
volume/total
volume)
PepGel,
where
synthetic
peptides
mixed
with
crosslinking
reagents
and
solution;
(2)
67%
PepGel;
(3)
80%
PepGel.
Each
sample
was
loaded
12
µg
incubated
PBS.
Released
quantified
by
ELISA
at
1
h,
6
1,
2,
4,
7,
10,
14,
21
days.
To
explore
how
influence
release,
gel
porosities,
swelling
ratios,
mechanical
properties
three
quantitatively
analyzed.
The
results
showed
that
encapsulated
exhibited
sustained
over
21-day
experiment,
while
PepGels
demonstrated
significantly
lower
rates
compared
after
day
7.
Higher
histological
porosity
correlated
increased
rates.
Conversely,
ratio
elastic
modulus
than
formulations.
In
conclusion,
this
indicates
crosslinked
can
control
vitro
modulating
porosity,
ratio,
properties.
Encapsulation
offers
pattern
vitro;
if
replicated
vivo,
could
mitigate
adverse
effects
associated
burst
clinical
applications.
