Integrated metabolomics and transcriptomics reveal the role of calcium sugar alcohol in the regulation of phenolic acid biosynthesis in Torreya grandis nuts DOI Creative Commons

Qiandan Xie,

Zhengchu Jiang,

Chenliang Yu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 5, 2024

Abstract Background：Torreya grandis, a prominent tree species of the autochthonous subtropical region China, possesses drupe-like fruit containing nut that is rich in nutrients and bioactive compounds. However, effect calcium (Ca2+) sugar alcohol (CSA), newly developed chelated Ca2+-fertilizer, on secondary metabolism phenolics T. grandis nuts largely unknown, for which transcriptomic metabolomic analysis was carried out. Results: Transcriptome sequencing detected 47,064 transcripts, several phenolic acid biosynthesis pathway-related genes were identified. Correlation showed four transcription factors, WRKY12, AP2-1, AP2-3, AP2-4, positively associated with accumulation acids. Furthermore, binding AP2-1 to HCT promoter confirmed using yeast one hybrid dual-luciferase assays. expression Nicotiana enhanced total flavonoid content. Conclusions: Our results indicated new regulatory module, Ca2+–AP2–HCT, involved regulation revealed, expanding understanding role Ca2+ fertilizers plant metabolism.

Language: Английский

Enhancing Germination and Growth of Chrysanthemum Synthetic Seeds Through Iron Oxide Nanoparticles and Indole-3-Acetic Acid: Impact of Treatment Duration on Metabolic Activity and Genetic Stability DOI Creative Commons
Dariusz Kulus, Alicja Tymoszuk, Katarzyna Gościnna

et al.

Nanotechnology Science and Applications, Journal Year: 2025, Volume and Issue: Volume 18, P. 139 - 155

Published: March 1, 2025

This study investigated the effects of pure iron oxide nanoparticles (Fe3O4 NPs), citrate-stabilized (Fe3O4CA and indole-3-acetic acid (IAA), applied at various time regimes, on germination, growth, ex vitro development chrysanthemum synthetic seeds. The genetic metabolic stability plants was also assessed. Nodal segments Chrysanthemum × morifolium /Ramat./ Hemsl. 'Richmond', with a single axillary bud, were encapsulated in 3% calcium alginate addition IAA (1 mg·L-1) and/or NPs (7.7 mg·L-1). seeds cultured for 30 or 60 days water-agar medium then transplanted to greenhouse further analyses. Results indicated that Fe3O4CA singularly significantly enhanced germination rates (83.33-92.18%) compared IAA- NP-free control (56.67-64.18%), regardless treatment time. simultaneous use promoted longer shoot after but showed negative extended exposure. same combination improved rooting efficiency alone. Supplementation acclimatization younger had variable older plants. Leaf growth metrics treatment, yet no significant differences observed leaf dimensions days. content flavonoids, anthocyanins, chlorophyll affected by exposure duration. Biochemical analyses revealed increased total polyphenol antioxidant capacity (FRAP, ABTS) treated plants, particularly NPs. Start codon targeted (SCoT) polymorphisms among confirming their stability. found seeds, while maintaining stability, although prolonged negatively plant metrics.

Language: Английский

Citations

0
Nanoparticle-Plant Interactions: Physico-chemical characteristics, Application Strategies, and Transmission Electron Microscopy-Based Ultrastructural Insights, with a focus on Stereological Research DOI
Hana Ďúranová, Samuel Kšiňan, Lenka Kuželová

et al.

Chemosphere, Journal Year: 2024, Volume and Issue: 363, P. 142772 - 142772

Published: July 4, 2024

Language: Английский

Citations

2
Nanoparticles in the Field: Sowing Innovation to Harvest a Sustainable Future DOI

Eliete A. Alvin,

W.S. Ribeiro, Anna Victória Bernardes e Borges

et al.

IntechOpen eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: March 26, 2024

The incorporation of nanoparticles into sensors or with antimicrobial and fertilizer properties in agriculture signifies a paradigm shift toward accuracy sustainability. This chapter shows the numerous uses nanoparticle-based agribusiness. Their innovative contribution to promoting eco-friendly practices is examined. A bounty advancements that maximize yields contribute sustainable agricultural future promised by these bactericidal nanoparticles, sensor technologies, enhanced fertilizers, which sow seeds creativity. discussion explores potential transform farming practices, diminish environmental harm, cultivate progressive, eco-conscious landscape. Nanoparticle-based provide data for informed decision-making, protect crops from harmful pathogens, nanotechnology enhances fertilizers nutrient delivery plant uptake.

Language: Английский

Citations

0
Integrated metabolomics and transcriptomics reveal the role of calcium sugar alcohol in the regulation of phenolic acid biosynthesis in Torreya grandis nuts DOI Creative Commons

Qiandan Xie,

Zhengchu Jiang,

Chenliang Yu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 5, 2024

Abstract Background：Torreya grandis, a prominent tree species of the autochthonous subtropical region China, possesses drupe-like fruit containing nut that is rich in nutrients and bioactive compounds. However, effect calcium (Ca2+) sugar alcohol (CSA), newly developed chelated Ca2+-fertilizer, on secondary metabolism phenolics T. grandis nuts largely unknown, for which transcriptomic metabolomic analysis was carried out. Results: Transcriptome sequencing detected 47,064 transcripts, several phenolic acid biosynthesis pathway-related genes were identified. Correlation showed four transcription factors, WRKY12, AP2-1, AP2-3, AP2-4, positively associated with accumulation acids. Furthermore, binding AP2-1 to HCT promoter confirmed using yeast one hybrid dual-luciferase assays. expression Nicotiana enhanced total flavonoid content. Conclusions: Our results indicated new regulatory module, Ca2+–AP2–HCT, involved regulation revealed, expanding understanding role Ca2+ fertilizers plant metabolism.

Language: Английский

Citations

0