Differential regulation of heparan sulfate biosynthesis in fibroblasts cocultured with normal vs. cancerous prostate cells DOI Creative Commons
Elvira V. Grigorieva, Anastasia V. Strokotova, Ingemar Ernberg

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 9, 2024

Heparan sulfate proteoglycans (HSPGs) regulate a wide range of biological activities in both physiological and pathological conditions. Altered expression or deregulated function HSPGs their heparan (HS) chains significantly contribute to carcinogenesis as well crucially depends on the functioning complex system HS biosynthetic/modifying enzymes termed “GAGosome”. Here, we aimed at investigating profile cell culture model stroma-epithelial crosstalk searching for transcription factors potentially related regulation genes involved. Coculture BjTERT-fibroblasts with normal PNT2 human prostate epithelial cells resulted significant downregulation (2-4-fold) transcriptional activity metabolism-involved ( EXT1/2, NDST1/2, GLCE, HS2ST1, HS3ST1/2, HS6ST1/2, SULF1/2, HPSE ) types, whereas coculture cancer (LNCaP, PC3, DU145) demonstrated no interchanges. Human Transcription Factor RT 2 Profiler PCR array manual RT-PCR verification supposed FOS, MYC, E2F, SRF, NR3C1 potential candidates and/or coordination biosynthesis. Taken together, biosynthetic fibroblasts during might be controlled by intercellular communication, reflecting adaptation these each other. The is attenuated abrogated if interact making independent limiting effects fibroblasts, thus contributing possibility unlimited growth progression. Overall, data demonstrate an ability cell-cell interactions affect biosynthesis-involved genes.

Language: Английский

Differential regulation of heparan sulfate biosynthesis in fibroblasts cocultured with normal vs. cancerous prostate cells DOI Creative Commons
Elvira V. Grigorieva, Anastasia V. Strokotova, Ingemar Ernberg

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 9, 2024

Heparan sulfate proteoglycans (HSPGs) regulate a wide range of biological activities in both physiological and pathological conditions. Altered expression or deregulated function HSPGs their heparan (HS) chains significantly contribute to carcinogenesis as well crucially depends on the functioning complex system HS biosynthetic/modifying enzymes termed “GAGosome”. Here, we aimed at investigating profile cell culture model stroma-epithelial crosstalk searching for transcription factors potentially related regulation genes involved. Coculture BjTERT-fibroblasts with normal PNT2 human prostate epithelial cells resulted significant downregulation (2-4-fold) transcriptional activity metabolism-involved ( EXT1/2, NDST1/2, GLCE, HS2ST1, HS3ST1/2, HS6ST1/2, SULF1/2, HPSE ) types, whereas coculture cancer (LNCaP, PC3, DU145) demonstrated no interchanges. Human Transcription Factor RT 2 Profiler PCR array manual RT-PCR verification supposed FOS, MYC, E2F, SRF, NR3C1 potential candidates and/or coordination biosynthesis. Taken together, biosynthetic fibroblasts during might be controlled by intercellular communication, reflecting adaptation these each other. The is attenuated abrogated if interact making independent limiting effects fibroblasts, thus contributing possibility unlimited growth progression. Overall, data demonstrate an ability cell-cell interactions affect biosynthesis-involved genes.

Language: Английский

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