Spike proteins of coronaviruses activate mast cells for degranulation via stimulating Src/PI3K/AKT/Ca 2+ intracellular signaling cascade DOI Creative Commons
Shuang Zhang,

Chu-Lan Xu,

Jingjing Wang

et al.

Journal of Virology, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

ABSTRACT Mast cells (MCs) are strategically located at the interface between host and environment. The non-allergic functions of MCs in immunosurveillance against pathogens have been recently underscored. However, activation by may beneficially or detrimentally regulate immune inflammation to combat promote pathogen invasion. We others conclusively demonstrated that serve as a crucial mediator induction hyperinflammation initiated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), leading substantial tissue damage across multiple organs murine nonhuman primate models. Whereas precise mechanism underlying virus-induced MC degranulation remains largely elusive, our previous findings indicated binding Spike proteins cellular receptors is sufficient elicit for rapid degranulation. This study aims corroborate ubiquity coronavirus-induced elucidate intracellular signaling pathways mediate upon protein receptors. Our transcriptome analysis revealed stimulations with range Spike/RBD viral particles coronavirus. Notably, interaction these triggered src kinase, member Src Family Kinases (SFKs). activation, turn, stimulated PI3K/AKT pathway, resulting an accumulation calcium ions. These ions subsequently facilitated microtubule-dependent granule transport, ultimately promoting In summary, this elucidates virus-triggered has potential aid development MC-targeted antiviral therapeutic strategies. IMPORTANCE mast (MCs), variety viruses, intricately linked pathogenesis. unknown. study, we demonstrate investigate process. reveal triggers kinase downstream facilitate

Language: Английский

Spike proteins of coronaviruses activate mast cells for degranulation via stimulating Src/PI3K/AKT/Ca 2+ intracellular signaling cascade DOI Creative Commons
Shuang Zhang,

Chu-Lan Xu,

Jingjing Wang

et al.

Journal of Virology, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

ABSTRACT Mast cells (MCs) are strategically located at the interface between host and environment. The non-allergic functions of MCs in immunosurveillance against pathogens have been recently underscored. However, activation by may beneficially or detrimentally regulate immune inflammation to combat promote pathogen invasion. We others conclusively demonstrated that serve as a crucial mediator induction hyperinflammation initiated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), leading substantial tissue damage across multiple organs murine nonhuman primate models. Whereas precise mechanism underlying virus-induced MC degranulation remains largely elusive, our previous findings indicated binding Spike proteins cellular receptors is sufficient elicit for rapid degranulation. This study aims corroborate ubiquity coronavirus-induced elucidate intracellular signaling pathways mediate upon protein receptors. Our transcriptome analysis revealed stimulations with range Spike/RBD viral particles coronavirus. Notably, interaction these triggered src kinase, member Src Family Kinases (SFKs). activation, turn, stimulated PI3K/AKT pathway, resulting an accumulation calcium ions. These ions subsequently facilitated microtubule-dependent granule transport, ultimately promoting In summary, this elucidates virus-triggered has potential aid development MC-targeted antiviral therapeutic strategies. IMPORTANCE mast (MCs), variety viruses, intricately linked pathogenesis. unknown. study, we demonstrate investigate process. reveal triggers kinase downstream facilitate

Language: Английский

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