The effects of Qingchang Ligan formula on hepatic encephalopathy in mouse model: results from gut microbiome-metabolomics analysis DOI Creative Commons
Ziwei Yang, Shu‐Hui Liu,

Feili Wei

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Aug. 16, 2024

Background Hepatic encephalopathy (HE) is a neurological disorder resulting from advanced liver injury. HE has high mortality rate and poor prognosis. The pathogenesis of still unclear, which led to the lack satisfactory specific treatment method. There increasing evidence that intestinal flora affects communication between gut brain in HE. Adjusting had beneficial effect on recent studies, Qingchang Ligan formula (QCLG) been shown previous studies regulate metabolites. In this study, we established thioacetamide-induced mouse model evaluate protective QCLG explore its potential mechanism, also demonstrated dysbiosis involved Methods Mice were intraperitoneally injected with thioacetamide (TAA, 150 mg/kg) induce Additionally, they orally administered Formula at dose 6.725 g/kg·d for seven days, while control mice received an equal volume saline via gavage. Subsequently, samples subjected 16S ribosomal ribonucleic acid (rRNA) gene sequencing, high-performance liquid chromatography-mass spectrometry (LC-MS), RNA-sequencing (RNA-seq) analysis. Result improved weight loss, cognitive impairment, function scores, blood ammonia, expression interleukin-6 (TNF-α), Interleukin-1β (IL-1β), (IL-6) induced by Moreover, increased levels indicators, including alanine aminotransferase (ALT), aspartate (AST), serum TNF-α, IL-1β, IL-6. RNA sequencing revealed Oscillibacter, Colidextribacter , Helicobacter TAA-induced fecal samples. Also, abundance Bifidobacterium decreases contrast, significantly restored microbial community. Metabolomics indicated significant differences some metabolites among normal control, treatment, groups, 5-methoxytryptophan, Daidzein, Stercobilin, Plumieride (PLU). Conclusion can alleviate neuroinflammation prevent caused injury regulating models.

Language: Английский

Disulfiram Reduces Atherosclerosis and Enhances Efferocytosis, Autophagy, and Atheroprotective Gut Microbiota in Hyperlipidemic Mice DOI Creative Commons
C. Alicia Traughber,

Kara Timinski,

Ashutosh Prince

et al.

Journal of the American Heart Association, Journal Year: 2024, Volume and Issue: 13(8)

Published: April 2, 2024

Pyroptosis executor GsdmD (gasdermin D) promotes atherosclerosis in mice and humans. Disulfiram was recently shown to potently inhibit GsdmD, but the vivo efficacy mechanism of disulfiram's antiatherosclerotic activity is yet be explored.

Language: Английский

Citations

12
Far-Infrared Therapy Based on Graphene Ameliorates High-Fat Diet-Induced Anxiety-Like Behavior in Obese Mice via Alleviating Intestinal Barrier Damage and Neuroinflammation DOI
Jinshui Zhang, Shuo Li,

Xin Cheng

et al.

Neurochemical Research, Journal Year: 2024, Volume and Issue: 49(7), P. 1735 - 1750

Published: March 26, 2024

Language: Английский

Citations

10
Evaluation of the anti-atherosclerotic effect for Allium macrostemon Bge. Polysaccharides and structural characterization of its a newly active fructan DOI

Pei Lin,

Qiqi Wang, Qi Wang

et al.

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 340, P. 122289 - 122289

Published: May 22, 2024

Language: Английский

Citations

9
Therapeutic Potential of Capsaicin against Cyclophosphamide-Induced Liver Damage DOI Open Access
Mohammad Firoz Alam,

Ahmed O. Ajeibi,

Majed H. Safhi

et al.

Journal of Clinical Medicine, Journal Year: 2023, Volume and Issue: 12(3), P. 911 - 911

Published: Jan. 24, 2023

Cyclophosphamide (CPM) is a classical alkylating agent used in different cancer chemotherapy regimens and restricted due to severe adverse effects, including hepatotoxicity. Natural or plant-derived antioxidants such as capsaicin were utilized this study examine the hepatoprotective benefits against cyclophosphamide-induced The rats divided into five groups: normal control group, toxic group (CPM), an intraperitoneal injection of single dose 200 mg/kg b.w. on fourth day, pretreated with two doses CPS (10 mg 20 b.w.) orally for six consecutive days, administration day treatment. fifth was administered highest (20 days. After 24 h CPS, anesthetized, blood collected, serum enzyme toxicity evaluated. sampling euthanasia all animals, liver isolated further histopathological examination. results revealed that markers (AST, ALT, ALP, BLI) significantly increased after CPM administration, but subsequently restored treatment both doses. In addition, lipid peroxidation (MDA), inflammatory cytokines (IL-1β, TNF-α), apoptotic (Caspase-3) increased, antioxidant enzymes (GSH, CAT, SOD) decreased it re-established by However, effectively protected CPM-induced architects tissues. conclusion, attenuates hepatotoxicity via modulating oxidative stress, signals, cytokine pathway. Therefore, could play significant role supplement during patients.

Language: Английский

Citations

19
A Review of the Health Benefits of Kimchi Functional Compounds and Metabolites DOI Open Access
Hyun Ju Kim,

Min Kwon,

Hyelyeon Hwang

et al.

Microbiology and Biotechnology Letters, Journal Year: 2023, Volume and Issue: 51(4), P. 353 - 373

Published: Nov. 14, 2023

Language: Английский

Citations

16
Capsaicin: A Potential Treatment to Improve Cerebrovascular Function and Cognition in Obesity and Ageing DOI Open Access
Tammy Thornton, Dean E. Mills, Edward Bliss

et al.

Nutrients, Journal Year: 2023, Volume and Issue: 15(6), P. 1537 - 1537

Published: March 22, 2023

Impaired cognition is the primary symptom of dementia, which can lead to functional disability and reduced quality life among an increasingly ageing population. Ageing associated with increased oxidative stress, chronic low-grade systemic inflammation, endothelial dysfunction, reduces cerebrovascular function leading cognitive decline. Chronic inflammatory conditions, such as obesity, exacerbate this decline beyond normal predispose individuals neurodegenerative diseases, dementia. Capsaicin, major pungent molecule chilli, has recently demonstrated improvements in animal models via activation transient receptor potential vanilloid channel 1 (TRPV1). Capsaicin-induced TRPV1 adiposity, well improves function, all are cognition. This review examines current literature on capsaicin Capsimax, a supplement gastrointestinal irritation compared capsaicin. Acute treatment improve animals. However, studies adequately assessing effects humans do not exist. Capsimax may be potentially safe therapeutic intervention for future clinical trials testing

Language: Английский

Citations

15
Dietary Capsaicin Exacerbates Gut Microbiota Dysbiosis and Mental Disorders in Type 1 Diabetes Mice DOI Open Access
Xiaohui Zhang,

Houjia Hu,

Yue Zhang

et al.

Nutrients, Journal Year: 2025, Volume and Issue: 17(3), P. 593 - 593

Published: Feb. 6, 2025

Background/Objectives: Diabetes mellitus is often accompanied by mental health complications, including anxiety, depression, and cognitive decline. Recent research suggested that capsaicin, the active component of chili peppers, may influence health. This study aimed to determine effect dietary capsaicin on disorders in a type 1 diabetes (T1D) mouse model, while also exploring potential involvement microbiota-gut-brain axis. Methods: We induced T1D mice using streptozotocin (STZ) administered diet supplemented with 0.005% for five weeks. Behavioral assessments, open field test (OFT), tail suspension (TST), forced swimming (FST), elevated plus maze (EPM) test, Morris water (MWM) were conducted evaluate depressive anxiety-like behaviors as well function. Targeted untargeted metabolomics analyses performed assess neurotransmitter levels hippocampus serum metabolites, 16S rRNA sequencing was utilized analyze gut microbiota composition. Intestinal barriers determined western blot detection tight junction proteins ZO-1 occludin. Results: Dietary exacerbated anxiety depressive-like along declines mice. Capsaicin reduced diversity beneficial bacteria, broad-spectrum antibiotic treatment further intensified depression behaviors. Metabolomic analysis indicated disrupted metabolic pathways related tryptophan phenylalanine, leading decreased neuroprotective such kynurenic acid, hippurate, butyric acid. Additionally, diminished expression occludin, indicating increased intestinal permeability. Conclusions: aggravates disturbances diabetic mice, thereby worsening

Language: Английский

Citations

0
Capsaicin and TRPV1: A Novel Therapeutic Approach to Mitigate Vascular Aging DOI Creative Commons

Xingyu Cui,

Jun‐Kun Zhan

Aging and Disease, Journal Year: 2025, Volume and Issue: unknown, P. 0 - 0

Published: Jan. 1, 2025

Vascular aging and its associated diseases represent a principal cause of mortality among the global elderly population, making mitigation vascular significant aspiration for humanity. This article explores intersection nature health, focusing on role natural plant, pepper, bioactive compound, capsaicin, in combating aging. By examining molecular cellular mechanisms as well phenotypic alterations blood vessels, we offer comprehensive review effects capsaicin receptor, transient receptor potential vanilloid 1 (TRPV1), within We propose that may serve medication with to slow progress could constitute new strategy treat related disease.

Language: Английский

Citations

0
Capsiate attenuates atherosclerosis by activating Nrf2/GPX4 pathway and reshaping the intestinal microbiota in ApoE −/− mice DOI Creative Commons
Yongbin Shen, Chuanqi Zhang, Xue Jiang

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

ABSTRACT Atherosclerosis (AS) is the basis of cardiovascular diseases (CVDs) and remains major contributor to death worldwide. Capsiate derived from sweet pepper fruit exhibits numerous pharmacological activities. The objective this study was elucidate protective role capsiate in atherosclerosis by examining its effect underlying regulatory pathways. Here, we showed that treatment alleviates atherosclerosis-prone apolipoprotein E-deficient (ApoE −/− ) mice. We found effectively reduced plaque area body weight compared Model group. inhibited inflammatory response downregulating phosphoinositide 3-kinase/protein kinase B/nuclear factor-κB pathway. Additionally, further investigation indicated could regulate lipid levels mice via reducing expressions 3-hydroxy-3-methylglutaryl coenzyme A reductase low-density lipoprotein receptor, increasing expression recombinant cytochrome P450 7A1. Furthermore, activated transient receptor potential vanilloid subfamily member 1 ApoE fed a high-fat diet. microbial sequencing demonstrated administration significantly regulated gut microbiota disturbance increased some beneficial bacterial ( Lachnospiraceae NK4A136 group) Human umbilical vein endothelial cells (HUVECs) were exposed oxidized (ox-LDL) stimulate atherosclerotic damage vitro . Our revealed ox-LDL-induced HUVECs injury inflammation. investigated effects on ferroptosis vivo ; it exhibited anti-ferroptosis through regulating nuclear factor erythroid 2-related 2/glutathione peroxidase 4 Interestingly, ML385 reversed HUVECs. Taken together, our findings suggest promising use small-molecule drug for AS related CVDs. IMPORTANCE has been inhibit fat accumulation, promote energy metabolism, exhibit anti-inflammatory antioxidative properties. However, there still no mouse models. first report reshaping structure AS, explore mechanism improvement AS. In study, alleviate diet-induced inhibiting response, improving serum profiles, activating pathway, suppressing ferroptosis. Moreover, reported as mediators therapy animal Therefore, these may provide robust experimental support clinical treatment.

Language: Английский

Citations

0
Gut microbiota-derived UDCA enhanced by metformin inhibits FXR to activate autophagy against MCD diet-induced NAFLD in mice DOI
Xiujie Liu,

Hongxia Sun,

Qing Han

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 153, P. 114471 - 114471

Published: March 22, 2025

Language: Английский

Citations

0