Elsevier eBooks, Journal Year: 2023, Volume and Issue: unknown, P. 293 - 301
Published: Nov. 30, 2023
Language: Английский
Biotechnology and Bioengineering, Journal Year: 2023, Volume and Issue: 120(8), P. 2056 - 2071
Published: July 13, 2023
Abstract Synthetic biology has mainly focused on introducing new or altered functionality in single cell systems: primarily bacteria, yeast, mammalian cells. Here, we describe the extension of synthetic to nematodes, particular well‐studied model organism Caenorhabditis elegans , as a convenient platform for developing applications multicellular setting. We review transgenesis techniques well application principles construct nematode gene switches and genetic devices control motility. Finally, discuss potential engineered nematodes.
Language: Английский
Citations
4
Journal of Food Science, Journal Year: 2023, Volume and Issue: 88(10), P. 4289 - 4304
Published: Sept. 7, 2023
Food allergy (FA) is acknowledged as a significant public health and food safety issue, due to its manifestation an amplified immune reaction antigens. Recently, probiotics within Lactobacillus Bifidobacterium have been highlighted promising strategy against allergic disease by modulating the balance of Th1/Th2 responses. However, allergy-alleviating effects probiotic Leuconostoc mesenteroides strains are unknown. Therefore, this study investigated potentials eleven L. on in vitro evaluating expression patterns interferon-gamma (IFN-γ) (Th1 cytokine) interleukin-4 (IL-4) (Th2 mesenteric lymph node-derived lymphocytes from ovalbumin (OVA)-sensitized mice. Among strains, WHH1141 incubation caused highest IFN-γ/IL-4 ratio. Oral administration (1 × 109 CFU/mL) OVA-induced FA mouse model for 40 days improved weight loss pathological symptoms normalized serum immunoglobulin E levels. Meanwhile, elevated gene expressions cytokines (IL-4, IL-5, IL-13) tight-junction proteins (ZO-1 Occludin) levels histamine jejunum were restored WHH1141. Furthermore, reversed reduced gut microbial diversity short-chain fatty acid (SCFA) levels, specifically increased Bacteroidota abundance, decreased Firmicutes abundance Overall, these findings suggest that exerts FA-alleviating mice, which involved with inhibition jejunal Th2 responses modulation microbiome composition SCFA productions. PRACTICAL APPLICATION: may be considered approach mitigation symptoms.
Language: Английский
Citations
2
Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 442 - 461
Published: Jan. 1, 2024
Language: Английский
Citations
0
Allergies, Journal Year: 2024, Volume and Issue: 4(4), P. 192 - 217
Published: Nov. 1, 2024
Food allergies (FAs) represent a significant and growing global health issue, with increasing prevalence across different age groups. This review provides comprehensive analysis of the epidemiology, mechanisms, risk factors involved in FA development. Currently, FAs are estimated to affect 2% general population, higher rates children (~8%). However, these figures may be inaccurate because reliance on self-reported data immunoglobulin E (IgE) testing, which not reflect clinically confirmed cases. Environmental genetic factors, including exposure bacterial toxins, dietary habits, gut microbiota, play critical roles Specifically, Staphylococcus aureus enterotoxins implicated disrupting intestinal barriers enhancing immune sensitization allergenic proteins. dysregulation promotes Th2 responses compromises regulatory T cell function, crucial elements allergy pathogenesis. As continues rise, there is pressing need for accurate diagnostic tools, heightened public awareness, effective prevention strategies. Further research needed elucidate specific role toxins other environmental development advance clinical management approaches.
Language: Английский
Citations
0
Elsevier eBooks, Journal Year: 2023, Volume and Issue: unknown, P. 293 - 301
Published: Nov. 30, 2023
Language: Английский
Citations
0