Unraveling symbiotic microbial communities, metabolomics and volatilomics profiles of kombucha from diverse regions in China

Food Research International, Journal Year: 2023, Volume and Issue: 174, P. 113652 - 113652

Published: Nov. 2, 2023

Language: Английский

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Cholecystectomy Significantly Alters Gut Microbiota Homeostasis and Metabolic Profiles: A Cross-Sectional Study

Nutrients, Journal Year: 2023, Volume and Issue: 15(20), P. 4399 - 4399

Published: Oct. 17, 2023

Cholecystectomy (CCE) is a standard clinical treatment for conditions like gallstones and cholecystitis. However, its link to post-CCE syndrome, colorectal cancer, nonalcoholic fatty liver disease has raised concerns. Additionally, studies have demonstrated the disruptive effects of CCE on gut microbiota homeostasis bile acid (BA) metabolism. Considering role in regulating host metabolic immune pathways, use dietary probiotic intervention strategies maintain stable ecosystem after could potentially reduce associated risks. Inter-study variations made it challenging identify consistent patterns CCE, prerequisite targeted interventions. In this study, we first meta-analyzed 218 raw 16S rRNA gene sequencing datasets determine structural functional changes CCE. Our results revealed significant alterations microbiota's structure function due Furthermore, identified characteristic with by constructing random model classifier. validation cohort, classifier achieved an area under receiver operating curve (AUC) 0.713 0.683 when distinguishing between healthy groups at family genus levels, respectively. Further, fecal metabolomics analysis that also substantially modified profile, including decreased short-chain levels disrupted BA Importantly, patterns, particularly excessive fat total energy intake, influenced profile post-CCE. These habits were further enrichment related metabolism increased intestinal inflammation conclusion, our study specific profiles It potential following provides theoretical basis modulating using long-term

Language: Английский

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Impact of cholecystectomy on the gut-liver axis and metabolic disorders

Clinics and Research in Hepatology and Gastroenterology, Journal Year: 2024, Volume and Issue: 48(7), P. 102370 - 102370

Published: May 9, 2024

Language: Английский

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Biliary-intestinal anastomosis leads to alterations in intestinal flora and its flora metabolites and increases the risk of long-term postoperative complications: a case-control study

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 26, 2025

Pancreaticoduodenectomy (PD) is a major surgical intervention that encompasses the resection of multiple organs and reconstruction digestive tract, with reconstructive procedures including pancreatico-enteric, bilioenteric, gastroenteric anastomoses. Prior research has documented high incidence long-term complications following PD, which significantly impact patient prognosis survival, however, underlying mechanisms remain elusive. Evidence from previous studies suggests biliary-intestinal anastomosis modifies biliary tract anatomy, altering bile flow into gut potentially affecting microbiota its metabolites. Given close association between infections alterations in microbiota, we hypothesize changes intestinal flora metabolites post-PD may be critical factor development complications. The objective this study to investigate whether during PD induces metabolites, turn increase risk postoperative This included 17 patients who underwent procedure (group T) 20 sex- age-matched controls did not N), group T were stratified those (complication group) without (non-complication Faecal samples collected all subjects DNA was extracted using 16S rRNA gene sequencing analyse composition faecal detect 1. Alpha diversity analysis two sample groups indicated trend towards lower microbial abundance Group relative N, no significant differences observed Shannon Simpson indices. 2. At genus level, exhibited markedly higher levels Escherichia-Shigella, Veillonella, Enterobacter, while showing Blautia Bifidobacterium compared N subjects. Analysis Spearman's correlation degree genera showed negative Escherichia shigella Blautia. Veillonella positive both Enterobacter. In addition, each other. 3. Subsequent comparative bacterial complication non-complication revealed elevated Escherichia-Shigella as group. 4. metabolomic L-palmitoylcarnitine, arachidic acid PG 13:0_15:0 increased group, whereas 3-isopropylmalic decreased 5. KEGG pathway identified nine crucial metabolic pathways associated these shifts: starch sucrose metabolism, steroid hormone biosynthesis, caffeine citric cycle, riboflavin sulfur biosynthesis valine, leucine, isoleucine, well pyruvate metabolism ABC transporter protein pathways. anastomosis, performed part pancreaticoduodenectomy, shifts flora. Increased promote after anastomosis. Biliary-intestinal leads patient's Intestinal their contribute through

Language: Английский

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Gut microbiota differences, metabolite changes, and disease intervention during metabolic - dysfunction - related fatty liver progression

World Journal of Hepatology, Journal Year: 2025, Volume and Issue: 17(3)

Published: March 25, 2025

In the current era, metabolic dysfunction-associated steatotic liver disease (MASLD) has gradually developed into a major type of chronic that is widespread globally. Numerous studies have shown gut microbiota plays crucial and indispensable role in progression MASLD. Currently, become one important entry points for research this disease. Therefore, aim review to elaborate on further associations between MASLD, including changes differences healthy diseased liver. Meanwhile, considering fatty steatohepatitis are abnormal pathological states development exhibits different degrees fibrosis (such as mild severe fibrosis) during progression, we also conduct comparison these use them markers progression. It reveals production action mechanisms short-chain acids bile brought about by microbiota, impact lipopolysaccharide from Gram-negative bacteria addition, regulation inhibition related factors probiotics (including new-generation probiotics) will be explored, which help monitor patients with compositions future carry out personalized targeted therapies microbiota. This achieve progress preventing combating

Language: Английский

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Multi-omics analysis reveals dynamic changes in gut microbiota and metabolites associated with cholecystectomy and post-cholecystectomy diarrhea

Published: April 1, 2025

Language: Английский

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Clinical perspectives on post-cholecystectomy syndrome: a narrative review

Annals of Medicine, Journal Year: 2025, Volume and Issue: 57(1)

Published: April 30, 2025

Post-cholecystectomy syndrome (PCS) is a complex condition characterized by persistent or new symptoms following gallbladder removal, affecting up to 47% of patients. Despite being recognized since 1947, there still no consensus on its etiology, diagnosis, and treatment. This narrative review explores the multifactorial etiology PCS, including biliary extra-biliary factors, varied clinical manifestations. A systematic literature search was conducted using keywords like 'etiology', 'clinical manifestations', 'diagnostic challenges', 'management strategies'. The covers traditional diagnostic methods, recent insights into pathophysiology, current management approaches, such as dietary modifications, pharmacological treatments, endoscopic interventions, with focus patient selection. PCS presents significant challenges due diverse presentations lack standardized therapeutic protocols. Effective starts careful selection before cholecystectomy prevent unnecessary surgeries reduce postoperative complications. Future research should aim refine criteria develop predictive models for identifying at-risk Personalized strategies incorporating genetic, biological, factors are essential improving outcomes. An integrated, patient-centered approach crucial addressing complexities enhancing quality life affected

Language: Английский

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Gut Microbiota-Targeted Intervention of Hyperlipidemia Using Monascus-Fermented Ginseng

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(5), P. 661 - 661

Published: April 30, 2025

Background/Objectives: Hyperlipidemia (HLP) encompasses a spectrum of poorly understood lipid metabolism disorders that are frequently overlooked or misdiagnosed, potentially leading to multiple complications. While the gut microbiota has been implicated in HLP pathogenesis, causal relationships and molecular mechanisms remain elusive. This study aimed investigate therapeutic Monascus-fermented ginseng (MFG) on through modulation explore treatment potential via fecal transplantation (FMT). Methods: The MFG-modulated was transplanted into mice. Systemic evaluations, including serum biochemical parameter detection, histopathological section analysis, 16S rRNA sequencing, metabolomics, were conducted assess efficacy identify associated metabolic pathways. Results: FMT significantly improved profiles, reduced body weight, attenuated hepatic accumulation Mechanistically, it enhanced cholesterol excretion fatty acid β-oxidation while suppressing lipogenic regulators, concurrently promoting primary-to-secondary bile conversion. Gut analysis revealed MFG intervention effectively normalized Firmicutes/Bacteroidetes ratio enriched beneficial microbiota. Conclusions: These findings demonstrate FMT’s value management provide new perspectives utilizing fermented herbal medicines for reprogramming.

Language: Английский

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Cassiae semen extract ameliorates hyperlipidemia in rats by modulating lipid metabolism and FcγR-mediated immune regulation

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 28, 2025

Introduction: Cassiae Semen Extract (CSE) shows promise in treating hyperlipidemia, although its underlying mechanisms are not yet fully understood. This study aimed to investigate the effects of CSE on hyperlipidemia rats and explore potential involved. Methods: Hyperlipidemic were induced by a high-fat diet (HFD) treated with CSE. Serum, liver, fecal samples analyzed through biochemical assays, histopathological examination, 16S rRNA sequencing, KEGG pathway analysis, Western blot. Results: treatment effectively alleviated imbalances tissue damage HFD. sequencing revealed that improved gut microbiota dysbiosis increased abundance. Pathological analysis showed reduced hepatic lipid accumulation, mitigating liver damage. suggested beneficial may involve Fc gamma receptor (FcγR)-mediated phagocytosis, immune activation influencing homeostasis inflammation. blot further indicated regulate metabolism via Sterol Regulatory Element-Binding Protein-1c (SREBP-1c) Peroxisome Proliferator-Activated Receptor Alpha (PPARα), while reducing inflammation MAPK signaling pathway. Discussion: ameliorate modulating disorders, metabolism, FcγR-mediated regulation, providing therapeutic approach for diseases associated metabolic dysfunction However, in-depth studies required elucidate these mechanisms.

Language: Английский

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EPRCN exerts neuroprotective function by regulating gut microbiota and restoring gut immune homeostasis in Alzheimer's disease model mice

Journal of Alzheimer s Disease, Journal Year: 2025, Volume and Issue: unknown

Published: May 5, 2025

Background No effective drug treatment is currently available for Alzheimer's disease (AD), highlighting the urgent need to develop efficient therapeutic options. We have developed a formula based on medicine and food homology (MFH) consisting of egg yolk oil, perilla seed raphani cinnamon noni puree (EPRCN), demonstrated that it can treat AD by alleviating neuroinflammation oxidative stress. However, whether EPRCN improve regulating gut microbiota remains unknown. Objective The current study aimed evaluate effect neuroprotection. Methods 16S rRNA sequencing was used assess structure microbiota. Hematoxylin-eosin (HE) staining, qRT-PCR, ELISA were inflammation. Detected indexes associated with cholinergic dysfunction neuronal damage investigate neuroprotective effects EPRCN. Results gene analysis revealed remodeled microbiota, inhibited metabolic disorders, promoted CoA biosynthesis in scopolamine-induced mice. ameliorates inflammation activating anti-inflammatory pathway. results further indicated improved inhibiting activity acetylcholinesterase restoring receptors. Additionally, administration suppressed loss elevated brain derived neurotrophic factor expression hippocampus. Correlation found alteration several microbes Conclusions These findings suggest may serve as promising dietary intervention treating microbiota-gut-brain axis exerting function.

Language: Английский

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