Dynamics alteration of the gut microbiota and faecal metabolomes in very low or extremely low birth weight infants: a Chinese single-center, prospective cohort study DOI Creative Commons
Ling Liu,

Chaohong Chen,

YeShan Li

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 23, 2024

Objective The aim of this study is to comprehensively investigate the temporal dynamics faecal gut microbiota and metabonomics in early postnatal with a focus on very low or extremely birth weight (VLBW/ELBW) infants. Methods We collected samples from 157 VLBW/ELBW infants at three time points: days 1, 14, 28 prospective cohort study. microbial diversity, abundance, composition, metabolomic analyses were determined using 16S rRNA sequencing liquid chromatography tandem mass spectrometry (LC-MS/MS). Microbiome functional conducted utilizing PICRUSt2. ecological association networks employed interactions between identify core genus within birth, as well unveil correlations taxa metabolites. Result (1) alpha diversity significantly decreased D1 D28, accompanied by an interrupted trajectory lacking obligate anaerobes. At phylum level, RNA results showed increase Proteobacteria decrease Firmicutes Bacteroidota D28. there was relative abundance Staphylococcus , Acinetobacter Ureaplasma Klebsiella Enterococcus emerging most abundant genera. (2) analysis revealed total 561 metabolic markers that exhibited significant distinct alterations D14. (3) Ecological higher degree compared those D14 Additionally, Enterobacter major contributors co-occurring network these points. (4) Steroid hormone biosynthesis, including tetrahydrocortisone, androsterone glucuronide, androstenedione etiocholanolone after birth. Conclusion have successfully demonstrated dysbiosis subsequent its 4 weeks postpartum Monitoring promptly rectifying stages may represent potential therapeutic strategy.

Language: Английский

Pathogen-specific alterations in intestinal microbiota precede urinary tract infections in preterm infants: a longitudinal case-control study DOI Creative Commons

Luyang Hong,

Yihuang Huang,

Junyan Han

et al.

Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)

Published: April 1, 2024

Urinary tract infections (UTIs) are among the most common late-onset in preterm infants, characterized by nonspecific symptoms and a pathogenic spectrum that diverges from of term infants older children, which present unique diagnostic therapeutic challenges. Existing data on role gut microbiota UTI pathogenesis this demographic limited. This study aims to investigate alterations fecal calprotectin levels their association with development UTIs hospitalized infants. A longitudinal case-control was conducted involving admitted between January 2018 October 2020. Fecal samples were collected weekly analyzed for microbial profiles levels. Propensity score matching, accounting key perinatal factors including age antibiotic use, utilized match UTI-diagnosed those non-UTI counterparts. Among 151 studied, 53 diagnosed UTI, predominantly caused Enterobacteriaceae (79.3%) Enterococcaceae (19.0%). Infants showed significantly higher abundance these families compared both Gram-negative positive pathogens, respectively. Notably, there significant pre-UTI increase pathogen-specific taxa later UTIs, offering high predictive value early detection. Shotgun metagenomic sequencing further confirmed dominance specific species revealed altered virulence factor associated Klebsiella aerogenes Escherichia coli infections. Additionally, decline observed preceding onset, particularly cases Enterobacteriaceae. The onset offer novel insight into promising biomarkers potentially enhancing timely management infection. However, validation larger cohorts is essential confirm findings.

Language: Английский

Citations

5
Breastmilk polyamines modify gut gene expression in children at three months of age DOI Creative Commons
María Sánchez-Campillo, Antonio Gázquez, María Teresa Pastor-Fajardo

et al.

Clinical Nutrition, Journal Year: 2025, Volume and Issue: 46, P. 80 - 87

Published: Jan. 22, 2025

Language: Английский

Citations

0
Maternal cobalt concentration and risk of spontaneous preterm birth: the role of fasting blood glucose and lipid profiles DOI Creative Commons
Junhua Huang,

Wei Zheng,

Aili Wang

et al.

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: Feb. 1, 2024

Introduction Spontaneous preterm birth (SPB) is a significant cause of neonatal mortality, yet its etiology remains unclear. Cobalt, an essential trace element, might be risk factor for SPB. This study aims to investigate the relationship between maternal serum cobalt concentration and SPB, clarify role blood lipids fasting glucose (FBG) in this relationship. Methods We conducted nested case-control within Beijing Birth Cohort Study. Serum samples were obtained from 222 pregnant women with SPB 224 controls during first (7–13 weeks pregnancy) third trimesters (32–42 pregnancy). was determined using inductively coupled plasma mass spectrometry (ICP-MS). Fasting levels detected fully automated biochemical immunoassay instrument. Logistic regression models linear established explore association women, test mediating effect lipids. Results found that mothers similar trimester, values 0.79 (0.58–1.10) ng/mL 0.75 (0.51–1.07) ng/mL, respectively. However, increased 0.88 (0.59–1.14) 0.84 (0.52–1.19) In logistic model, when considering trimester pregnancy, after adjusting ethnicity, pre-pregnancy body index (BMI), age, education, income, parity, it observed medium level (0.63–1.07 ng/ml) had negative correlation The odds ratio (OR) 0.56, 95% confidence interval 0.34–0.90 p -value 0.02. suggests range played protective against Additionally, FBG pregnancy partial intermediary role, accounting 9.12% association. no trimester. Conclusion During intermediate appear offer protection playing role. To further optimal concentrations different populations, multi-center larger sample size necessary. exploring specific mechanism FBG’s could provide valuable insights improving prevention

Language: Английский

Citations

0
The Association between Delayed Gut Microbiota Maturity in Pre-Term Infants and the Feeding Intolerance—A Pilot Study DOI Creative Commons

Ya-Chi Hsu,

Ming‐Chih Lin,

Katharina Ardanareswari

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(3), P. 539 - 539

Published: Feb. 28, 2024

This study compared gut (fecal) microbiota profiles between pre-term and full-term infants, assuming that infants without feeding intolerance would have similar to those of infants. A total 13 (gestational age < 37 weeks, birthweight ≤ 2500 g) 10 were included. The assigned the tolerance (FT) group (n = 7) if their daily intake exceeded 100 mL/kg/day at two weeks after birth, or (FI) 6). Microbial DNA from weekly fecal samples was analyzed. significantly different (p 0.0001), as well FT FI groups 0.0009). had more diversity, with higher concentrations facultative anaerobes such Bifidobacteriaceae Lactobacteriaceae. group’s matured over four levels digestion-related bacteria, while pathogens. In group, a significant difference observed first second no differences noted week third fourth weeks. delay in development infants’ may be associated FI.

Language: Английский

Citations

0
Advances in Enteral Nutrition Strategies for Preterm Infants DOI Open Access

Zhen Hu,

Xiaofang Zhu

Journal of Biosciences and Medicines, Journal Year: 2024, Volume and Issue: 12(11), P. 286 - 298

Published: Jan. 1, 2024

Language: Английский

Citations

0
Host-microbiota interactions in the infant gut revealed by daily faecal sample time series DOI Open Access

Nienke van Beek,

Iiris Katavisto,

Markku Lehto

et al.

Microbiome Research Reports, Journal Year: 2024, Volume and Issue: 4(1)

Published: Dec. 27, 2024

Aim: This study aims to explore the interplay between host immune factors and gut microbiota in human infants vivo using time-series daily stool samples identify biomarkers of host-microbe interactions. Methods: 216 faecal collected from aged 5-6 or 11-12 months were analysed for composition, total bacterial load, function. Results: We identified indications microbial stimulation eosinophil cationic protein (ECP), IgA, calprotectin (Cal), intestinal alkaline phosphatase (IAP), Bactericidal/permeability-increasing (BPI) at 6 12 months, as well lipocalin 2 (LCN2), lactoferrin (LTF), alpha-defensin-5 only months. The associations biomarker concentrations population growth primarily positive mostly negative suggesting increasing regulation with age. exceptions IAP, which was predictive declining populations both time points, Cal, whose changed Conclusion: There is an age-associated development correlation pattern concentrations, that interactions change during early development. Albumin appeared a potential marker permeability, while LCN2 seemed correlate transit time. Mucin degradation decrease Mucin2 IAP emerged potentially important regulators infant gut. demonstrates utility bacteria profiling analysing system provides evidence infants.

Language: Английский

Citations

0
Dynamics alteration of the gut microbiota and faecal metabolomes in very low or extremely low birth weight infants: a Chinese single-center, prospective cohort study DOI Creative Commons
Ling Liu,

Chaohong Chen,

YeShan Li

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 23, 2024

Objective The aim of this study is to comprehensively investigate the temporal dynamics faecal gut microbiota and metabonomics in early postnatal with a focus on very low or extremely birth weight (VLBW/ELBW) infants. Methods We collected samples from 157 VLBW/ELBW infants at three time points: days 1, 14, 28 prospective cohort study. microbial diversity, abundance, composition, metabolomic analyses were determined using 16S rRNA sequencing liquid chromatography tandem mass spectrometry (LC-MS/MS). Microbiome functional conducted utilizing PICRUSt2. ecological association networks employed interactions between identify core genus within birth, as well unveil correlations taxa metabolites. Result (1) alpha diversity significantly decreased D1 D28, accompanied by an interrupted trajectory lacking obligate anaerobes. At phylum level, RNA results showed increase Proteobacteria decrease Firmicutes Bacteroidota D28. there was relative abundance Staphylococcus , Acinetobacter Ureaplasma Klebsiella Enterococcus emerging most abundant genera. (2) analysis revealed total 561 metabolic markers that exhibited significant distinct alterations D14. (3) Ecological higher degree compared those D14 Additionally, Enterobacter major contributors co-occurring network these points. (4) Steroid hormone biosynthesis, including tetrahydrocortisone, androsterone glucuronide, androstenedione etiocholanolone after birth. Conclusion have successfully demonstrated dysbiosis subsequent its 4 weeks postpartum Monitoring promptly rectifying stages may represent potential therapeutic strategy.

Language: Английский

Citations

0