Effects of Propolis Supplementation on Gut Microbiota and Uremic Toxin Profiles of Patients Undergoing Hemodialysis DOI Creative Commons
Larissa Fonseca, Márcia Ribeiro, Júnia Schultz

et al.

Toxins, Journal Year: 2024, Volume and Issue: 16(10), P. 416 - 416

Published: Sept. 25, 2024

Background: Propolis possesses many bioactive compounds that could modulate the gut microbiota and reduce production of uremic toxins in patients with chronic kidney disease (CKD) undergoing hemodialysis (HD). This clinical trial aimed to evaluate effects propolis on profile toxin plasma levels HD patients. These are secondary analyses from a previous double-blind, randomized study, 42 divided into two groups: placebo group received 400 mg green extract/day for eight weeks. Indole-3 acetic acid (IAA), indoxyl sulfate (IS), p-cresyl (p-CS) were evaluated by reversed-phase liquid chromatography, cytokines investigated using multiplex assay (Bio-Plex Magpix®). The fecal composition was analyzed subgroup (n = 6) commercial kit DNA extraction. V4 region 16S rRNA gene then amplified polymerase chain reaction (PCR) short-read sequencing Illumina NovaSeq PE250 platform subgroup. Forty-one completed 20 21 group. There positive correlation between IAA TNF-α (r 0.53, p 0.01), IL-2 0.66, 0.002), pCS IL-7 0.46, 0.04) at baseline. No significant changes observed values after intervention. Despite not being significant, microbial evenness richness increased following Counts Fusobacteria species showed IS, while counts Firmicutes, Lentisphaerae, Proteobacteria phyla negatively correlated IS. Two months supplementation did (IAA, p-CS) or change microbiota.

Language: Английский

Potential Strategies for Overcoming Drug Resistance Pathways Using Propolis and Its Polyphenolic/Flavonoid Compounds in Combination with Chemotherapy and Radiotherapy DOI Open Access
Nada Oršolić, Maja Jazvinšćak Jembrek

Nutrients, Journal Year: 2024, Volume and Issue: 16(21), P. 3741 - 3741

Published: Oct. 31, 2024

Conventional cancer treatments include surgical resection, chemotherapy, hyperthermia, immunotherapy, hormone therapy, and locally targeted therapies such as radiation therapy. Standard often require the use of multiple agents, which can activate nuclear factor kappa B (NF-κB) in tumor cells, leading to reduced cell death increased drug resistance. Moreover, agents also contributes added toxicity, resulting poor treatment outcomes. Cancer cells gradually develop resistance almost all chemotherapeutics through various mechanisms, efflux, alterations metabolism transport, changes signal transduction pathways, enhanced DNA repair capacity, evasion apoptosis, mutations, reactivation targets, interaction with microenvironment, cell-stroma interactions, epithelial–mesenchymal transition (EMT)-mediated chemoresistance, epigenetic modifications, metabolic alterations, effect stem (CSCs). Developing new strategies improve chemotherapy sensitivity while minimizing side effects is essential for achieving better therapeutic outcomes enhancing patients’ quality life. One promising approach involves combining conventional propolis its flavonoids. These natural compounds may enhance response reducing toxicity. Propolis components sensitize chemotherapeutic likely by inhibiting NF-κB activation, reprogramming tumor-associated macrophages (TAMs; an M2-like phenotype), thereby release matrix metalloproteinase (MMP)-9, cytokines, chemokines, vascular endothelial growth (VEGF). By TAMs, overcome EMT-mediated disrupt crosstalk between CSCs, inhibit maintenance stemness, reverse acquired immunosuppression, thus promoting antitumor mediated cytotoxic T-cells. This review highlights potential flavonoids modulate responsiveness modalities. The evidence suggests that novel incorporating could be developed positive cytotoxicity peripheral blood leukocytes, liver, kidney cells. Therefore, polyphenolic/flavonoid hold combination clinical types cancers.

Language: Английский

Citations

7
The antioxidant property of CAPE depends on TRPV1 channel activation in microvascular endothelial cells. DOI Creative Commons

Miltha Hidalgo,

Bárbara Railef,

Vania Rodríguez

et al.

Redox Biology, Journal Year: 2025, Volume and Issue: 80, P. 103507 - 103507

Published: Jan. 20, 2025

Caffeic acid phenethyl ester (CAPE) is a hydrophobic phytochemical typically found in propolis that acts as an antioxidant, anti-inflammatory and cardiovascular protector, among several other properties. However, the molecular entity responsible for recognising CAPE unknown, whether interaction involved developing antioxidant response target cells remains unanswered question. Herein, we hypothesized subfamily of TRP ion channels works recognizes at plasma membrane allows fast shift capacity intact endothelial (EC). By monitoring cytoplasmic Ca2+ microvascular EC model, compared calcium responses evoked by three structurally related compounds: caffeic ester, neochlorogenic acid. Only induced rapid transient nanomolar concentrations together with gradual increase sodium levels, suggesting activation non-selective cationic permeation membrane. Electrophysiological well pharmacological, RNA silencing assays confirmed involvement TRPV1 recognition ECs. Finally, demonstrated influx was necessary recording CAPE-induced redox changes, phenomenon captured real-time ECs expressing HyPer biosensor. Our data depict mechanism behind effect cells, connecting channels, increase, reduction disulfide bonds on This occurs within seconds to minutes contributes better understanding mechanisms underlying vasodilatory compounds interact vascular bed.

Language: Английский

Citations

0
Exploring Mechanism of Electroacupuncture in Modulating Neuroinflammation Based on Intestinal Flora and Its Metabolites DOI

Hai-min Ye,

Z.-L. Li,

Peng Zhang

et al.

Chinese Journal of Integrative Medicine, Journal Year: 2024, Volume and Issue: unknown

Published: July 23, 2024

Language: Английский

Citations

2
The double-layer emulsions loaded with bitter melon (Momordica charantia L.) seed oil protect against dextran sulfate sodium-induced ulcerative colitis in mice DOI

Yan Ma,

Wangting Zhou, Huiling Wang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 278, P. 134279 - 134279

Published: July 30, 2024

Language: Английский

Citations

2
Caffeic Acid Phenethyl Ester Administration Reduces Enterotoxigenic Bacteroides fragilis-Induced Colitis and Tumorigenesis DOI Creative Commons
Soonjae Hwang, Minjeong Jo, Ju-Eun Hong

et al.

Toxins, Journal Year: 2024, Volume and Issue: 16(9), P. 403 - 403

Published: Sept. 18, 2024

The human colonic commensal enterotoxigenic Bacteroides fragilis (ETBF) is associated with chronic colitis and colon cancer. ETBF colonization induces via the toxin (BFT). BFT secreted by cause inflammation E-cadherin cleavage/NF-κB signaling. promotes tumorigenesis interleukin 17A (IL-17A)/CXCL-dependent inflammation, but its bioactive therapeutics in ETBF-promoted remain unexplored. In current study, we investigated caffeic acid phenethyl ester (CAPE) murine model of tumorigenesis. this observed that CAPE treatment mitigated induced mice. Additionally, our findings indicate offers protective effects against ETBF-enhanced a mouse colitis-associated cancer azoxymethane (AOM) dextran sulfate sodium. Notably, decrease following administration correlates reduction expression IL-17A CXCL1 gastrointestinal tract. molecular mechanism for CAPE-induced protection ETBF-mediated mediated IL-17A/CXCL1, NF-κB activity intestinal epithelial cells. Our may serve as preventive agent development ETBF-induced colorectal (CRC).

Language: Английский

Citations

2
Effects of Propolis Supplementation on Gut Microbiota and Uremic Toxin Profiles of Patients Undergoing Hemodialysis DOI Creative Commons
Larissa Fonseca, Márcia Ribeiro, Júnia Schultz

et al.

Toxins, Journal Year: 2024, Volume and Issue: 16(10), P. 416 - 416

Published: Sept. 25, 2024

Background: Propolis possesses many bioactive compounds that could modulate the gut microbiota and reduce production of uremic toxins in patients with chronic kidney disease (CKD) undergoing hemodialysis (HD). This clinical trial aimed to evaluate effects propolis on profile toxin plasma levels HD patients. These are secondary analyses from a previous double-blind, randomized study, 42 divided into two groups: placebo group received 400 mg green extract/day for eight weeks. Indole-3 acetic acid (IAA), indoxyl sulfate (IS), p-cresyl (p-CS) were evaluated by reversed-phase liquid chromatography, cytokines investigated using multiplex assay (Bio-Plex Magpix®). The fecal composition was analyzed subgroup (n = 6) commercial kit DNA extraction. V4 region 16S rRNA gene then amplified polymerase chain reaction (PCR) short-read sequencing Illumina NovaSeq PE250 platform subgroup. Forty-one completed 20 21 group. There positive correlation between IAA TNF-α (r 0.53, p 0.01), IL-2 0.66, 0.002), pCS IL-7 0.46, 0.04) at baseline. No significant changes observed values after intervention. Despite not being significant, microbial evenness richness increased following Counts Fusobacteria species showed IS, while counts Firmicutes, Lentisphaerae, Proteobacteria phyla negatively correlated IS. Two months supplementation did (IAA, p-CS) or change microbiota.

Language: Английский

Citations

2